A kind of method for aseptic germination of Pseudomonas acuminate gentian seeds

Abstract

The invention provides a method for sterilely germinating gentianella acuta seeds. The method comprises the following steps: (1) washing the gentianella acuta seeds and putting the gentianella acuta seeds into a 20%-50% disinfection solution to be immersed and disinfected for 5-20 minutes; washing the seeds with sterile distilled water for 3 times to obtain the disinfected seeds; and (2) inoculating the disinfected seeds obtained by the step (1) into an MS culture medium to be cultured; and culturing the seeds under the conditions that the culture temperature is 23-27 DEG C, the illumination intensity is 1500lux and the illumination time is 12-14 hours per day for 30 days, germinating the seeds to obtain sterile test-tube plantlets, wherein 30g / L sucrose and 3.4g / L agar are added into the MS culture medium and the pH value of the culture medium is 5.8. With the adoption of the method for sterilely germinating the seeds, the germination rate is up to 85%-95%, the problem that the germination of gentianella acuta tissue culture seeds is difficult is solved, and the foundation is laid for sustainable utilization of gentianella acuta sources.

Description

technical field [0001] The invention relates to a plant seed germination method, in particular to a method for aseptic germination of pseudogentiana acupoints. Background technique [0002] Gentianella acuta (Michx.) Hulten is a plant of the genus Gentianella Moench. in the family Gentiananceae, also known as bitter gentian. Gentianaceae annual herb, the whole herb is used as medicine. Widely distributed in my country's Hebei, Northeast, Inner Mongolia, Shanxi, Shandong and other regions, the Soviet Union, Mongolia, and North America are also distributed. It is mostly used in Mongolian and Tibetan medicine. In Mongolian medicine, it is called "Agut-Qiqige". It has the effect of clearing away heat and detoxifying. Ewenki and Oroqen hunters have long used it to treat arrhythmia and other heart diseases with remarkable effects. It is the only plant in this genus that has the effect of treating heart disease, and it has an irreplaceable position in Mongolian medicine. [0003...

AI Technical Summary

Problems solved by technology

In the work of breeding and raising seedlings, it was found that the germination rate of Pseudomonas acupoints was low and the germination was not uniform during the sowing process, which seriously restricted the quality of the seedlings of Pseudomonas acuminates

Producing high-quality seedlings through tissue culture technology has become an effective way to produce seedlings of various plants, but when conducting research on tissue culture of P. The Key Factors of Mass Production of Seedlings by the Way of Culture