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Quality detection method of Chinese herb gentian

A detection method and technology of gentian can be used in pharmaceutical formulations, measuring devices, medical preparations containing active ingredients, etc., which can solve problems affecting the safety and effectiveness of clinical applications, and achieve high extraction efficiency and sample pretreatment process. simple effect

Inactive Publication Date: 2013-01-02
CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many varieties of traditional Chinese medicine gentian on the market now, and the chemical composition and content of each variety are different. If the chemical composition and content cannot meet the quality requirements, it will inevitably affect the safety and effectiveness of clinical application.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] (1) Preparation of test sample solution

[0015] Crush the traditional Chinese medicine gentian, pass through a 60-mesh sieve, weigh 50 mg of the crushed gentian, put it in a 50 ml conical flask, add 5 ml of methanol with a concentration of 50% by volume, and perform ultrasonic extraction once, 20 min / time, at a temperature of 20 ℃. Filter, combine the above extracts and concentrate to 1ml, dilute to 10ml with methanol, shake well, that is the test sample solution.

[0016] (2) Electrospray fingerprint of test sample

[0017] Take the test sample solution, dilute it 10 times with methanol, and analyze it by electrospray mass spectrometry. The experimental conditions of electrospray mass spectrometry are as follows: electrospray ionization source, spray voltage 3.5kV, capillary temperature 180°C, flow injection pump injection flow rate 2μl / min; scan range mass-to-charge ratio (m / z) 50-2000, using Spray mass spectrometry confirmed the species of secoiridoid glycosides....

Embodiment 2

[0022] (1) Preparation of test sample solution

[0023] Crush the traditional Chinese medicine gentian, pass through a 80-mesh sieve, weigh 200 mg of the crushed gentian, put it in a 50ml conical flask, add 20ml of methanol with a concentration of 100% by volume, and perform ultrasonic extraction for 3 times, 40min / time, at a temperature of 50°C . Filter, combine the above-mentioned extracts and concentrate to 2ml, dilute to 10ml with methanol, shake up, it is the test sample solution.

[0024] (2) Electrospray fingerprint of test sample

[0025] Take the test sample solution, dilute it 10 times with methanol, and analyze it by electrospray mass spectrometry. The experimental conditions of electrospray mass spectrometry are as follows: electrospray ionization source, spray voltage 5.5kV, capillary temperature 220°C, flow injection pump injection flow rate 8μl / min; scan range mass-to-charge ratio (m / z) 50-2000, using Spray mass spectrometry confirmed the species of secoirido...

Embodiment 3

[0030] (1) Preparation of test sample solution

[0031] Crush the traditional Chinese medicine gentian, pass through a 70-mesh sieve, weigh 150 mg of the crushed gentian, put it in a 50ml conical flask, add 15ml of methanol with a concentration of 75% by volume, perform ultrasonic extraction twice, 30min each time, and the ultrasonic extraction temperature is 35°C . Filtrate, combine the above extracts and concentrate to 1.5ml, dilute to 10ml with methanol, shake well, that is the test sample solution.

[0032] (2) Electrospray fingerprint of test sample

[0033]Take the test sample solution, dilute it 10 times with methanol, and analyze it by electrospray mass spectrometry. The experimental conditions of electrospray mass spectrometry are as follows: electrospray ionization source, spray voltage 4.5kV, capillary temperature 200°C, flow injection pump injection flow rate 6μl / min; scan range mass-to-charge ratio (m / z) 50-2000, using Spray mass spectrometry confirmed the spec...

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Abstract

The invention provides a quality detection method of Chinese herb gentian. The method comprises the following steps: processing the gentian by sample pretreatment means of grinding, sifting, methanol ultrasound extracting, fixing volume and filtering, then obtaining finger-print of split iridoid glycoside component by electrospray ionization mass spectrometry, determining the species of the splitiridoid glycoside component contained in the gentian according to the finger-print; measuring the content of the split iridoid glycoside compound by high performance liquid chromatography; synthesizing the finger-print and the content measurement data to evaluate the quality of the gentian. The quality detection method is accurate, quick, convenient and effective.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine, and in particular relates to a quality detection method of the traditional Chinese medicine gentian. technical background [0002] The traditional Chinese medicine Gentian is the dried product of Gentiana manshurica Kitag., Gentiana scabra Bge., Gentiana triflora Pall. or Gentiana rigescens Franch. Roots and rhizomes, the first three are commonly called "gentian", and the latter are commonly called "gentian". Among them, the striped gentian is also called the northeast gentian, and the gentian is also called the rough gentian. Gentian is cold in nature, bitter in taste, and belongs to the liver and gallbladder meridian. It has the effect of clearing away heat and dampness, and purging liver and gallbladder fire. For damp-heat jaundice, pruritus vulvae swelling, vaginal discharge, eczema itching, convulsions and deafness, convulsions and convulsions embolism. Gentian is a c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/515G01N27/68G01N30/36
Inventor 宋凤瑞齐静静邢俊鹏刘志强刘淑莹
Owner CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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