Screening method for preparation process of gentiana macrophylla
A technology of preparation process and screening method, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of loss of active ingredients, reduction of drug efficacy, etc., and achieve the effect of improving quality
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[0073] Preparation of the control solution: Take appropriate amount of gentiopicroside reference substance and loganinic acid reference substance, weigh them accurately, add methanol to prepare solutions containing 0.5 mg of gentiopicroside and 0.3 mg of loganinic acid per 1 ml, namely have to.
[0074] Preparation of the test solution: Get about 0.5 g of the powder of this product (passed through a No. 3 sieve), accurately weighed, put in a stoppered Erlenmeyer flask, add 20 ml of methanol precisely, and ultrasonically treat (power 500W, frequency 40kHz) for 30 minutes. Let it cool, then weigh it, make up the lost weight with methanol, shake well, filter, and take the filtrate to obtain the final product.
[0075] Determination method: Precisely draw 5-10 μl each of the two reference substance solutions and the test solution, inject it into the liquid chromatograph, measure it, and obtain it.
[0076] Calculated as a dry product, this product contains no less than 2.5% of th...
Embodiment
[0081] Gentiana process control point
[0082]
[0083] Horizontal scrolling spray moistening machine, rotary disc cutting machine, model QYJ-100, produced by Shanghai Nanjue Traditional Chinese Medicine Machinery Co., Ltd.; preparation flap dryer, multi-layer flap superconducting energy-saving dryer, model CJGD-20 , produced by Zhejiang Xinchang Great Wall Air-Conditioning Parts Co., Ltd.; drug screening machine, model SYJ-B, produced by Shanghai Nanjue Traditional Chinese Medicine Machinery Company.
[0084] Select 2 process links of infiltration and drying, 2 process conditions for each, 3 process levels for each, 3 test research object factors, set 3 repetitions, and achieve 81 test combinations, that is, use a horizontal rolling spray wetting machine Complete the infiltration of Gentiana chinensis, the test factors are infiltration water volume and infiltration time, each factor has 3 levels. Among them, the amount of infiltration water is selected as 0.15 times, 0.2 ...
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