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Alpine gentian herb HPLC detection method

A detection method, the technology of white flower gentian, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of complex varieties, lack of internal quality evaluation, etc., and achieve the effect of guaranteeing quality

Inactive Publication Date: 2015-11-25
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As far as the current situation of use is concerned, the original varieties of "Bangjian" are complex, and "Bangjian Gabao" is used in more than one kind, and there are cases where "Bangjian Enbao" is used instead of "Bangjian Gabao"
Modern Tibetan medical works and national and local standards contain a wide range of varieties, and the quality control methods are simple, lacking internal quality evaluation standards

Method used

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Experimental program
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Effect test

Embodiment 1

[0069] Embodiment 1 Detection method of the present invention

[0070] (1) Preparation of the test solution: take about 0.5 g of the sample powder (passed through a No. 3 sieve), accurately weigh it, put it in a stoppered Erlenmeyer flask, add 25 mL of methanol, weigh it, and ultrasonically process it (power 250w, frequency 50Hz ) for 30 minutes, take it out, let it cool, make up methanol to lose weight, shake well, filter, and take the continued filtrate to get final product;

[0071] (2) HPLC detection need testing solution:

[0072] The chromatographic conditions are as follows:

[0073] Chromatographic column: octadecylsilane bonded silica gel column Swell (4.6×250 mm, 5 μm, model: 1118254605).

[0074] Detection wavelength: 240nm.

[0075] Mobile phase: A phase is 0.2% phosphoric acid aqueous solution, B phase is methanol:.

[0076] Flow rate: 1 mL / min.

[0077] Column temperature: 30°C.

[0078] Injection volume: 10μL

[0079] The gradient elution program is:

[...

Embodiment 2

[0082] Embodiment 2 The screening of detection method chromatographic conditions of the present invention

[0083] 1. Screening of chromatographic columns

[0084] The inventor screened the following chromatographic columns: Diamonsil-C18 (Dima), Global (SP-120-5-C18-BIO), Welchrom-C18 (model: U15LP18425), Swell-C18.

[0085] Such as figure 1 with figure 2 As shown, when the chromatographic column is Welchrom-C18, Swell-C18, the resolution of each chromatographic peak of the obtained spectrum is good. The chromatographic column is finally determined to be Swell-C18.

[0086] 2. Screening of mobile phase

[0087] The preparation method of need testing solution is:

[0088] Get Gentiana daflora (DH-1) powder 0.5g, add methanol 25ml to extract, ultrasonic 30min, filter, take the subsequent filtrate as Gentiana daflora test solution;

[0089] Take Minxian gentian (MX-6) powder 0.5g, add methanol 25ml to extract, ultrasonic 30min, filter, take the subsequent filtrate as Minx...

Embodiment 3

[0109] Embodiment 3 The screening of need testing solution preparation method of the present invention

[0110] The inventor screened the preparation method of the test solution, including reflux extraction and ultrasonic extraction, and the extractant included methanol, ethanol and ethyl acetate.

[0111] The result is as Figure 7 As shown, when extracted with ethanol or ethyl acetate, the effect is not satisfactory. However, when extracting with methanol, regardless of reflux and ultrasonic treatment, the resolution of each chromatographic peak on the obtained spectrum is better, and the peak shape is better.

[0112] In summary, the best need testing solution preparation method of the present invention is as follows:

[0113] Take about 0.5 g of the sample powder (passed through a No. 3 sieve), accurately weigh it, put it in a stoppered Erlenmeyer flask, add 25 mL of methanol, weigh it, ultrasonically treat it (power 250w, frequency 50Hz) for 30 minutes, take it out, let...

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Abstract

The invention provides an alpine gentian herb HPLC detection method including the following operation steps: taking a to-be-detected medicinal material standard, and extracting with methanol, and preparing a test sample solution; injecting the test sample solution into a high performance liquid chromatograph, detecting, and building a standard fingerprint atlas; taking a to-be-detected medicinal material, obtaining a fingerprint atlas according to the method with the steps, and comparing the fingerprint atlas with the standard fingerprint atlas. The detection method has good degree of separation, has more peak appearing number, can distinguish multiple varieties of alpine gentian herb from confusion or counterfeit species, and provides a guarantee for the quality of the alpine gentian herb medicinal material.

Description

technical field [0001] The invention relates to an HPLC detection method of Gentiana candidum. Background technique [0002] (bāngjiān, "Bangjian"), "Bang" means "alpine meadow" in Tibetan, and "jian" means "decoration", that is, "the embellishment of alpine meadow". The Chinese name is gentian flower. "Jingzhu Materia Medica" is classified as flower medicine. According to different flower colors, it is often divided into three types: white, blue, and black. They are respectively "Bangjian Gabao" (Chinese name, white flower gentian), which has white flowers. It grows in cold areas of high mountains in late autumn, and its leaves are like Gentiana chinensis leaves. ; Stemless, four or five white flowers bloom from the ground, with red luster, and the bases of the flowers are conjoined. "Bangjian Enbao" (Blue Flower Gentian), with blue flowers, grows in very humid swamp grassland in early autumn, with small leaves and light blue flowers; "Bangjian Nabao" (Black Flower Gent...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 马逾英马羚古锐
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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