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Antioxidant active extract of snow chrysanthemum and preparation method thereof

A technology of antioxidant activity and extract, applied in the field of snow chrysanthemum antioxidant activity extract and its preparation, to achieve the effect of safe and reliable extraction process and good antioxidant effect

Inactive Publication Date: 2014-06-18
乌鲁木齐高原红菊生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there is no technical means to efficiently prepare the extract of snow chrysanthemum antioxidant activity at home and abroad. Therefore, there is no research report on the separation and extraction of snow chrysanthemum antioxidant active components using antioxidant activity as an indicator. It is important to develop

Method used

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  • Antioxidant active extract of snow chrysanthemum and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1, a snow chrysanthemum antioxidant active extract is obtained according to the following preparation method: first step, the snow chrysanthemum is crushed and passed through a 10-mesh sieve to a 50-mesh sieve, and 6 to 20 times the weight of the snow chrysanthemum is added The volume fraction is 70% to 95% ethanol aqueous solution, soak until thoroughly, extract 1 to 3 times, each extraction is 0.5 hours to 2 hours, the temperature of each extraction is 76 ° C to 80 ° C, after each extraction Filtration is carried out to obtain the filtrate, and the filtrates are combined and then concentrated under reduced pressure to obtain an ethanol extract; the second step is to add 1 to 4 times the weight of the ethanol extract to the ethanol extract and suspend it in water to obtain Suspension, adding petroleum ether to the suspension to extract 3 to 6 times to obtain petroleum ether raffinate, adding 1 to 4 times the weight of the ethanol extract of petroleum ether for e...

Embodiment 2

[0028] Example 2, a snow chrysanthemum antioxidant activity extract is obtained according to the following preparation method: the first step, the snow chrysanthemum is crushed and passed through a 10-mesh sieve or a 50-mesh sieve to obtain a snow chrysanthemum powder, and the snow chrysanthemum powder is added The ethanol aqueous solution with a volume fraction of 70% or 95% is extracted once or three times, and the time of each extraction is 0.5 hours or 2 hours, and the temperature is 76°C or 80°C. Add snow chrysanthemum to the crushed snow chrysanthemum for each extraction 6 times by weight or 20 times by weight of the pulverized product is an aqueous ethanol solution with a volume fraction of 70% or 95%, after each extraction, it is filtered to obtain a filtrate, and the filtrate is combined and then concentrated under reduced pressure to obtain an ethanol extract; the second step , add 1 or 4 parts by weight of water to the ethanol extract to suspend the ethanol extract at ...

Embodiment 3

[0029] Embodiment 3, as the preference of the foregoing embodiments, in the first step, the filtrate is combined and then concentrated under reduced pressure until the mass water content in the ethanol extract is 40% to 50%; or / and, in the first step, reduce Concentration under pressure is carried out at a temperature of 50°C to 70°C and a pressure of -0.06Mpa to -0.1Mpa.

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Abstract

The invention relates to the technical field of snow chrysanthemum extraction, and discloses an antioxidant active extract of snow chrysanthemum and a preparation method thereof. The antioxidant active extract of the snow chrysanthemum is prepared by the following preparation method: firstly, extracting by using an ethanol water solution after crushing the snow chrysanthemum and then carrying out vacuum concentration to obtain an ethanol extract; adsorbing by using macroporous resin, eluting by using an ethanol water solution, carrying out vacuum common peony and drying to obtain the antioxidant active extract of the snow chrysanthemum after extracting by petroleum ether, trichloromethane and ethyl acetate sequentially. The result of an experiment of the antioxidant active extract of the snow chrysanthemum obtained by the method in the ability of removing and restoring DPPH and ABTS free radicals proves that the antioxidant active extract of the snow chrysanthemum disclosed by the embodiment of the invention has good antioxidant action; the process disclosed by the invention is simple and convenient to operate, safe and reliable in extraction process, and is a technique capable of effectively extracting the antioxidant active extract of the snow chrysanthemum.

Description

technical field [0001] The invention relates to the technical field of snow chrysanthemum extraction, and relates to an antioxidant active extract of snow chrysanthemum and a preparation method thereof. Background technique [0002] With the improvement of people's living standards, various diseases caused by active oxygen and free radicals have seriously endangered human health, and have attracted widespread attention from all walks of life. In recent years, evaluating and screening natural resources with strong antioxidant activity has become a new trend in biology, medicine and food science research. [0003] Snow chrysanthemum, also known as "blood chrysanthemum", scientifically named Coreopsis tinetoria, is an annual herb of the genus Coreopsis in the Compositae family. It is mainly distributed in the Kunlun Mountains at an altitude of about 3000m in Hotan, Xinjiang. For a long time, Kunlun snow chrysanthemum has been drunk as scented tea by local residents, and it is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A61P39/06A23L1/30A23L33/105
Inventor 周巨幸王强刘亚亚杨洁曹宝勇李强张志强
Owner 乌鲁木齐高原红菊生物科技有限公司
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