45results about How to "Economic extraction" patented technology

The invention relates to a method for extracting acid-soluble collagen from fish skin and an acid-soluble collagen product extracted by the method. The method comprises the following steps: removing impure protein and fat of the fish skin; extracting the acid-soluble collagen, dialyzing, performing freeze-drying and the like. The acid-soluble collagen product extracted by the method keeps an integral structure and is not degradable; and the acid-soluble collagen product has a denaturation temperature similar to that of collagen from a large terrestrial animal, can be used as a substitute for the collagen from the large terrestrial animal, and has a potential application value.

The invention provides a rapid no-heating nucleic acid extraction kit based on a magnetic bead method. The kit comprises a solution containing magnetic beads, protease K, a polyA solution, deproteinizing liquid, washing liquid, eluant and lysate. The invention also provides a method for extracting nucleic acid by using the kit. The method includes the steps that the lysate and the magnetic beads are added to a sample and fully shaken for uniform mixing, and when lytic cells release nucleic acid, the nucleic acid is captured with the magnetic beads; then, the specific deproteinizing liquid andwashing liquid are adopted for washing respectively to remove protein, salt ions and other impurities on the surfaces of the magnetic beads; finally, the specific eluant is used for rapidly eluting the nucleic acid at room temperature. Compared with a conventional nucleic acid extraction kit based on a magnetic bead method, the kit has the advantages that the steps are simple and rapid, and the whole process only takes about 15 min; besides, heating is not needed, so that the probability of sample room contamination possibly caused by aerosol is reduced; no chloroform phenol or other toxic reagents are used, so that the kit is safe and free of pollution.

The invention discloses a method for recovering sulfur from atmospheric-pressure oxygen-rich leached residues of lead-zinc ores, and particularly relates to the recovery of sulfur in lead-zinc ore leached residues through vacuum distillation, belonging to the field of reclamation and reuse of solid waste resources. The method comprises the following steps of: placing atmospheric-pressure oxygen-rich leached residues, subjected to drying, milling and screening pretreatment, of lead-zinc ores into a vacuum distillation furnace, heating the residues to 200-400 DEG C, and controlling the pressure of the inside of the furnace to 600-10000 Pa, so that sulfur in the leached residues is sublimated and volatilized; pumping sulfur-containing vapor out of the distillation furnace by a vacuum pump; and cooling the sulfur-containing vapor so as to obtain a sulfur product. After the distillation is completed and the sulfur is completely volatilized, high-boiling-point residues are discharged out of the distillation furnace in a solid powder form. In the whole process, the furnace is internally sealed, thereby avoiding the oxidation and environmental pollution of elemental sulfur. The method is simple in technological process and equipment, high in sulfur recovery rate and product quality, low in energy consumption, strong in adaptability to raw materials and pollution-free, so that the method disclosed by the invention is an economic and efficient sulfur extraction method and can realize the zero discharge of waste smelting residues in a lead-zinc wet process, thus the method has broad application prospects.

The present invention provides an alcohol-free virus nucleic acid extraction kit based on a magnetic bead method. The alcohol-free virus nucleic acid extraction kit comprises a lysate, a magnetic beadsolution, proteinase K, a polyA solution, a deproteinized solution, a washing solution and a buffer solution. The present invention also provides a method for extracting nucleic acid by using the above kit. Firstly, a sample, the lysate and magnetic beads are mixed evenly, then nucleic acid molecules are captured while pyrolysis is conducted, and finally the special alcohol-free deproteinized solution and the washing solution are respectively used to remove impurities of proteins, salt ions and other impurities on surfaces of the magnetic beads. Compared with a conventional virus nucleic acidextraction method, the method is simple in operation steps, does not need single addition of binding liquid, realizes the simultaneous pyrolysis and binding, and co-extracts DNA and RNA; the whole operation process is free of alcohol or isopropyl alcohol, inhibitory substance residues are less, the experimental procedure is simple, and the whole process only takes about 40 minutes; and the method is safe and pollution-free, and free of toxic reagents of chloroform, phenol, etc., and can be operated manually or on an automation platform.

The invention discloses a process for recovering sulfur, lead, zinc and silver from oxygen-enriched directly leached residues of zinc concentrates or lead-zinc mixed ores, which comprises the following steps: adding the oxygen-enriched directly leached residues of zinc concentrates or lead-zinc mixed ores, which are not processed or subjected to drying, grinding and screening pretreatment, into a heating device in which mixed liquid of a sulfur extracting agent and an additive metered in a liquid-solid ratio of 8-15 is filled, after heating for reaction, filtering the obtained object while the obtained object is hot, and cooling and then filtering filter liquor so as to obtain a sulphur product; after filter residues are washed by using a sulfur extracting agent and ethanol, returning the obtained product to a lead system to recover lead and silver; after washing liquid is volatilized, cooled and recovered, returning the obtained object to use; after the sulfur extracting agent is filtered, regenerating, and returning the obtained object to a sulfur extracting process so as to cyclically participate in the reaction; and after a zinc-containing solution is filtered, returning the obtained product to a direct leaching system. The process is simple in process and equipment and high in sulfur recovery rate and product quality, has no pollution to the environment, both can realize the efficient and economic extraction of sulfur in floating sulfur residues, and can realize the respective efficient and economic recovery of zinc, lead and silver in floating sulfur residues.

The present invention relates to the use of Indian Green Mussel (Perna viridis) as a source of anti-HIV activity. More particularly, the present invention relates to the use of an extract of Indian Green Mussel (Perna viridis) as a source of anti-HIV activity in vitro.

The present invention relates to a method for extracting lithium from a solution containing lithium, and more particularly, to a method for extracting lithium from a solution containing lithium, comprising the steps of: preparing a solution containing lithium including carbonate ions; obtaining a first precipitate by concentrating the solution containing lithium in order to allow the lithium concentration to be 2.481 g / L or less; performing solid-liquid separation of the concentrated solution containing lithium and the first precipitate; precipitating dissolved lithium as lithium phosphate by inputting a phosphor supplying material into the concentrated solution containing lithium; obtaining a second precipitate by re-concentrating a filtrate with the precipitated lithium phosphate in order to allow the lithium concentration to be 2.481 g / L or less; performing solid-liquid separation of the re-concentrated filtrate and the second precipitate; and precipitating the dissolved lithium as lithium phosphate by inputting nuclear particles into the re-concentrated filtrate.

A process for extracting metal values from ores or residues is disclosed. The process mentioned above is mainly suitable for aluminoferrous ores such as bauxite, titanoferrous ores such as ilmenite, or residues such as red mud waste. The process involves pulverizing the ore and / or residue and mixing with a carbonaceous material, followed by smelting the iron values and slag in the mixture to yield molten iron and oxides of aluminum and titanium. The process is simple, cost-effective, and provides effective extraction of high purity metal values.

The present invention relates to a method of economical extraction of magnesium, boron and calcium, while minimizing the loss of lithium, from a lithium bearing solution. More specifically, the present invention provides a method for economical extraction of magnesium, boron, and calcium, while minimizing the loss of lithium, from a lithium bearing solution comprising the steps of: (a) adding an alkali in the lithium bearing solution to precipitate magnesium hydroxide; (b) absorbing boron ions on the surface of the magnesium hydroxide by adjusting the pH to about 8.5 to about 10.5; and (c) simultaneously extracting magnesium and boron by filtering the magnesium hydroxide absorbed with the boron ions from the lithium bearing solution.

The invention relates to a method for extracting titanium dioxide from titaniferous materials, such as blast furnace slag, ilmenite or titanium slag, and the like, comprising three steps: dissolving blast furnace slag in a hydrogen bromide solution, processing filter materials by alkaline, separating TiO2 and SiO2, and the like, wherein hydrogen bromide can be circularly used, and therefore, the sufficient hydrogen bromide and raw materials are added into a beginning period to start reaction. The method for extracting titanium dioxide from titaniferous materials is economical, efficient and eco-friendly.

The invention provides a kit for rapidly extracting genomes of animal excrement by virtue of a CTAB method. The kit is utilized for carrying out pretreating the excrement, splitting microbial cells in the excrement, dissociating DNA, abstracting impurity protein, adsorbing DNA by virtue of a DNA adsorption column, removing impurities and eluting DNA; after cells are disrupted, protein impurities are removed by virtue of a CTAB solution, meanwhile, genome DNA is adsorbed by virtue of a silicone mold adsorption column, and finally, the residual protein impurities are eluted by virtue of special deproteinization liquid and rinsing liquid. A conventional animal excrement microbial genome extraction method needs to be finished within 14 hours or longer, and the genomes are easily degraded due to the long-playing operation. Compared with the conventional animal excrement microbial genome extraction method, the kit has the advantages that the animal excrement genomes can be extracted within only 4 hours, so that plenty of extraction time is saved, meanwhile, experimental steps are simplified, and the microbial genomes of the animal excrement can be relatively effectively, rapidly and economically extracted.

The invention discloses a kit and an extraction method for rapidly extracting RNA of animal fecal microorganism. The kit mainly adopts a silicone mold adsorption column to absorb the RNA of the fecal microorganism, and then adopts a peculiar protein removing solution and a rinsing solution to wash away impurities such as proteins and the like. Compared with the conventional method for extracting the RNA of the animal fecal microorganism, the extracting method by using the kit saves plenty of time for extraction and meanwhile simplifies experimental procedure, and can extract the RNA of the animal fecal microorganism more effectively, rapidly and economically.

Nickel entrained in the sulphide mineral pyrrhotite is engineered to dissolve in leaching acid in a two step procedure. First, a slurry of the mineral and the acid is activated by oxidation. This is done in a time T1 by electrolysis; or alternatively chemically, by adding e.g an oxidizing acid to the mineral. After activation, the slurry is then kept under anoxic conditions for a time T2. During T2, the sulphide starts to dissolve much more rapidly, and the rapid breakdown of the sulphide enables the nickel to dissolve and thus to be leached out of the mineral. The dissolved nickel is extracted from the leaching acid e.g by electro-winning.

The invention provides a method for rapidly extracting animal waste microbial genomes based on a paramagnetic particle method. According to the method for rapidly extracting the animal waste microbial genomes based on the paramagnetic particle method, microbial cells are broken through lysozyme, impurities are removed by use of a phenol chloroform isoamyl alcohol solution, and the silicon substrate magnetic bead is adopted to specifically adsorb the animal waste microbial genomes DNA. Compared with a conventional extracting method of the animal waste microbial genomes, the method for rapidly extracting the animal waste microbial genomes based on the paramagnetic particle method has the advantages that not only is a large amount of extracting time saved, but also experimental procedures are simplified, and the animal waste microbial genomes can be extracted more effectively, rapidly and economically.

The invention discloses an extraction method for microbial DNA in milk. The method comprises the following steps: adding 0.8 mL of a milk sample into 0.3 to 0.5 mL of an ES solution (EDTA with a pH value of 7.5 and concentration of 0.5 mol / L and SDS with concentration of 2%), carrying out uniform mixing and centrifugation and discarding supernatant; adding 0.8 to 1.2 mL of a PBS buffer solution, carrying out oscillation and shaking up, then carrying out centrifugation and discarding supernatant; adding 20 to 30 [mu]L of Staphylococcus lysozyme with a mass concentration of 0.02 mg / mL; adding 150 to 200 [mu]L of an aqueous Chelex-100 solution with a mass concentration of 10%, carrying out violent oscillation for 5 to 10 s and then carrying out heating in boiling water with a temperature of 100 DEG C for 8 to 12 min; and carrying out centrifugation and taking supernatant for a PCR reaction. The method provided by the invention employs a Chelex-100 process and can economically, rapidly, efficiently and directly extract bacterial DNA from the milk sample; and the bacterial DNA can be used for PCR detection of main pathogenic bacteria causing mastitis in cow.

The invention relates to an ishige okamurae polysaccharide extraction method. The method includes following steps: collecting, drying, crushing, pre-treating, mixing, extracting, centrifuging, concentrating and precipitating. Extraction through a high-pressure sterilizing pot and an ultrasonic water extraction method are combined, so that extraction rate of ishige okamurae polysaccharide is increased greatly. Content of extracted polysaccharide is higher due to the pretreatment step; water is adopted as a solvent to extract polysaccharide, penetrability to algae cells is high, extraction efficiency is high, and the water is safe and economical to use during production. Polysaccharide obtained by the method is yellowish white and odorless and has certain gel characteristics.

The invention discloses a method for extracting nonferrous metal from a waste circuit board. The method comprises the following steps that 1, the waste circuit board is pretreated so as to obtain fine-particle materials; 2, the fine-particle materials are subjected to gravity separation so as to obtain a heavy product; 3, a reducing agent, a chlorinating agent and the heavy product are subjected to heat treatment correspondingly; 4, valuable metal is subjected to a chlorination reaction; and 5, ferromagnetic materials are removed, and a concentrate of the nonferrous metal is obtained. According to the extracting method, the circuit board is crushed and heated, a mixture containing the nonferrous metal is obtained, emission of a large amount of harmful gas and solid waste is reduced, and thus the environment is protected; a wet-type shaking-table sorting method is adopted, and thus dust is avoided; and a combination of reaction material preheating and high-temperature chlorination is adopted, the chlorine retention capacity and the chlorination efficiency of the valuable metal are improved, the dosage of the chlorinating agent is decreased, the extracting process is simplified, andthe recycling cost is reduced.

The invention discloses a method for extracting valuable metal from low-content nonferrous metal. The method comprises the following steps: (1) pretreatment, (2) preheating, (3), high-temperature chloridizing roasting, and (4), trapping and recovery of valuable metal chloride. According to the chloridizing metallurgy method for which metal chloride is used for metal extraction, great advantages are provided when low-grade metal materials which are difficult to process are processed, and valuable metal can be extracted economically and efficiently, and the method is a universal technical scheme aiming at complex low-content nonferrous metal materials with various processing types, large resource quantities and complex ore types.

The present invention relates to a method for extracting lithium. The method comprising the steps of: preparing a lithium-containing solution containing an alkaline earth metal; controlling the activity of alkaline earth metal in the lithium-containing solution; and adding a phosphorus supply material to the lithium-containing solution to precipitate lithium phosphate.