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Preparation technology for producing collagen through enzymolysis of fish scales

A preparation process and collagen technology, applied in the field of collagen extraction, can solve the problems of difficulty in obtaining high content of low-molecular-weight collagen peptides, difficulty in obtaining low-molecular-weight collagen, and difficulty in removing pigment substances, etc. Absorption, strong activity, small molecular weight effect

Inactive Publication Date: 2015-06-24
NINGDE XIAWEI FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When chemical methods such as strong acid and strong alkali are used to hydrolyze collagen, the hydrolysis reaction is difficult to control, and it is difficult to obtain high-content low-molecular-weight collagen peptides
In addition, a large number of chemical substances are used, which affects food safety and causes serious environmental pollution.
It is difficult to obtain low-molecular-weight collagen by the biological hydrolysis method of hydrolyzing collagen with industrial enzymes
Although there are currently reports of low-molecular-weight collagen peptides obtained by enzymatically hydrolyzing collagen with complex enzymes, the low-molecular-weight collagen peptides obtained by enzymatically hydrolyzing collagen with complex enzymes are prone to unfocused molecular weight distribution or low-molecular-weight collagen peptides. The phenomenon that the content is too high, and it is difficult to remove the pigment substances that affect the appearance of collagen products, and the smell of fishy fish is strong

Method used

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  • Preparation technology for producing collagen through enzymolysis of fish scales
  • Preparation technology for producing collagen through enzymolysis of fish scales
  • Preparation technology for producing collagen through enzymolysis of fish scales

Examples

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preparation example Construction

[0053] A preparation process for enzymatic hydrolysis of fish scales to produce collagen, said method comprising the following steps:

[0054] (1) cleaning

[0055] Clean fish scales, remove sundries, and drain water;

[0056] (2) Alkali treatment

[0057] Pour fish scales into the enzymolysis tank, and add NaOH solution with a mass concentration percentage of 0.2%-0.5%, the mass volume concentration of fish scales and NaOH solution is 800-1500kg / m 3 ,

[0058] Add pure water to adjust the mass volume concentration of fish scales and NaOH solution to 300-440kg / m 3 , and adjust the temperature in the enzymolysis tank to 17-19°C, let it soak for 12-15 hours, filter out the NaOH solution, and wash the fish scales with pure water until neutral;

[0059] (3) Decalcification

[0060] Add an appropriate amount of acid solution into the enzymolysis tank, adjust the temperature in the enzymolysis tank to 17-19°C, soak the fish scales with the acid solution for 2-4 hours, filter ou...

Embodiment 1

[0087] Embodiment 1 (best embodiment)

[0088] A preparation process for enzymatic hydrolysis of fish scales to produce collagen, said method comprising the following steps:

[0089] (1) cleaning

[0090] Clean fish scales, remove sundries, and drain water;

[0091] (2) Alkali treatment

[0092] Pour fish scales into the enzymolysis tank, and add NaOH solution with a mass concentration percentage of 0.3%, the mass volume concentration of fish scales and NaOH solution is 1200kg / m 3 ,

[0093] Add pure water to make the mass volume concentration of fish scales and NaOH solution 370kg / m 3 , and adjust the temperature in the enzymolysis tank to 18°C, let it soak for 14 hours, filter out the NaOH solution, and wash the fish scales with pure water until neutral;

[0094] (3) Decalcification

[0095] Add disodium EDTA and citric acid into pure water at a mass ratio of 6.8:78 to prepare a citric acid solution with a mass concentration of 16.8%, and pour it into an enzymatic hydr...

Embodiment 2

[0117] A preparation process for enzymatic hydrolysis of fish scales to produce collagen, said method comprising the following steps:

[0118] (1) cleaning

[0119] Clean fish scales, remove sundries, and drain water;

[0120] (2) Alkali treatment

[0121] Pour fish scales into the enzymolysis tank, and add NaOH solution with a mass concentration percentage of 0.2%, the mass volume concentration of fish scales and NaOH solution is 800kg / m 3 ,

[0122] Add pure water so that the mass volume concentration of fish scales and NaOH solution is 300kg / m 3 , and adjust the temperature in the enzymolysis tank to 17°C, let it soak for 12 hours, filter out the NaOH solution, and wash the fish scales with pure water until neutral;

[0123] (3) Decalcification

[0124] Add disodium EDTA and citric acid to pure water at a mass ratio of 1:17 to prepare a citric acid solution with a mass concentration of 15%, and pour it into an enzymatic hydrolysis tank. The mass volume concentration of...

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Abstract

The invention relates to a preparation technology for producing collagen and in particular relates to a preparation technology for producing collagen through enzymolysis of fish scales, belonging to the technical field of collagen extraction. The preparation technology comprises the following steps of (1) washing, (2) alkali treatment, (3) decalcification, (4) cooking, (5) cooling, (6) primary enzymolysis, (7) secondary enzymolysis, (8) yeast fermentation of a fish scale collagen solution, (9) activated carbon adsorption reaction, (10) filtration, (11) concentration, (12) sterilization and (13) spray drying. The method provided by the invention not only can achieve the effect of obtaining collagen peptides with high contents and low molecular components from the fish scales but also can achieve the effects of removing pigment substances affecting the appearances of collagen products and reducing the peculiar fishy smell of collagen, thus improving the values of fish collagen products.

Description

technical field [0001] The invention relates to a preparation process for producing collagen, in particular to a preparation process for producing collagen by enzymatic hydrolysis of fish scales, and belongs to the technical field of collagen extraction. Background technique [0002] Collagen is the protein with the most content and widest distribution in the human body. It is the main protein component of connective tissue, accounting for about 25% of the total body protein. It is mainly distributed in animal skin, bone, cartilage, cornea, inner wall of blood vessels and other tissues . Collagen extracted from animal connective tissue is widely used in industries related to human health, such as food, cosmetics and medical treatment, due to its special protein structure and body affinity. At present, the collagen used in industrial production is mainly type I collagen, which is a high-molecular hard protein composed of three 1000 amino acid peptide chains. It is difficult ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 蔡述秋李庆华
Owner NINGDE XIAWEI FOOD
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