Method for preparing high-strength collagen tissue repair material product
A technology for repairing materials and collagen tissue, applied in medical science, prostheses, catheters, etc., can solve the problems of no significant improvement in mechanical strength, low mechanical strength such as tensile and compressive strength, and low collagen content, etc. Achieve the effect of increasing collagen solid content, improving mechanical properties, and increasing collagen solid content
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Embodiment 1
[0029] The selected collagen mother liquor with a concentration of 4 g / L is mixed with saturated sodium chloride solution and stirred continuously. The collagen gradually precipitates from the collagen solution. After the above solution is centrifuged at a speed of 10,000 rpm, the supernatant is poured off. , collect the precipitate, and then extrude for dehydration. The filter membrane pore size of the extrusion dehydration is 100 microns, the extrusion pressure is 5MPa, and the dehydration extrusion time is 20 minutes. Finally, a collagen gel with a solid content of about 80 mg / liter is obtained. The collagen gel was poured into a mold, and freeze-dried for 24 hours to obtain a repair material product with certain shape characteristics. Then put the above product into 0.25% glutaraldehyde solution for cross-linking for 12 hours and freeze-dry for 24 hours to obtain the final repair material product. The article has a compressive strength of about 12 MPa.
Embodiment 2
[0031] The selected collagen mother solution with a concentration of 6 g / L is mixed with saturated potassium chloride solution, and the collagen is gradually precipitated from the collagen solution. After the above solution is centrifuged at a speed of 15000 rpm, the supernatant is poured out. , collect the precipitate, and then extrude for dehydration. The filter membrane pore size of the extrusion dehydration is 50 microns, the extrusion pressure is 10MPa, and the dehydration extrusion time is 40 minutes. Finally, a collagen gel with a solid content of about 120 mg / liter is obtained. The collagen gel was poured into a mold, and freeze-dried for 24 hours to obtain a repair material product with certain shape characteristics. Then put the above product into 5.5% EDC solution for cross-linking for 12 hours and freeze-dry for 24 hours to obtain the final repair material product. The article has a compressive strength of about 20 MPa.
Embodiment 3
[0033] The selected concentration is 2 g / L of collagen mother liquor mixed with saturated magnesium chloride solution, stirring continuously, the collagen gradually precipitates from the collagen solution, after the above solution is centrifuged at 8000 rpm, the supernatant is poured, collected Precipitate, extrusion dehydration again, the filter membrane pore diameter of extrusion dehydration is 200 microns, extrusion pressure is 1MPa, and dehydration extrusion time is 15 minutes, finally obtains the collagen gel that solid content is about 40 mg / liter, the The collagen gel was poured into the mold and freeze-dried for 24 hours to obtain a repair material product with certain shape characteristics. Then put the above product into 5.5% EDC solution for cross-linking for 12 hours and freeze-dry for 24 hours to obtain the final repair material product. The article has a compressive strength of about 8 MPa.
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