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Method for preparing high-strength collagen tissue repair material product

A technology for repairing materials and collagen tissue, applied in medical science, prostheses, catheters, etc., can solve the problems of no significant improvement in mechanical strength, low mechanical strength such as tensile and compressive strength, and low collagen content, etc. Achieve the effect of increasing collagen solid content, improving mechanical properties, and increasing collagen solid content

Inactive Publication Date: 2009-10-28
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The collagen biomaterial prepared by the above-mentioned method is prepared by freeze-drying of the collagen solution, and the collagen solution is low in solubility in the solution, and the collagen content in the solution is small, so that the prepared collagen products are The mechanical strength such as tensile and compressive strength is low. Although cross-linking can be used, the final mechanical strength will not be significantly improved.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The selected collagen mother liquor with a concentration of 4 g / L is mixed with saturated sodium chloride solution and stirred continuously. The collagen gradually precipitates from the collagen solution. After the above solution is centrifuged at a speed of 10,000 rpm, the supernatant is poured off. , collect the precipitate, and then extrude for dehydration. The filter membrane pore size of the extrusion dehydration is 100 microns, the extrusion pressure is 5MPa, and the dehydration extrusion time is 20 minutes. Finally, a collagen gel with a solid content of about 80 mg / liter is obtained. The collagen gel was poured into a mold, and freeze-dried for 24 hours to obtain a repair material product with certain shape characteristics. Then put the above product into 0.25% glutaraldehyde solution for cross-linking for 12 hours and freeze-dry for 24 hours to obtain the final repair material product. The article has a compressive strength of about 12 MPa.

Embodiment 2

[0031] The selected collagen mother solution with a concentration of 6 g / L is mixed with saturated potassium chloride solution, and the collagen is gradually precipitated from the collagen solution. After the above solution is centrifuged at a speed of 15000 rpm, the supernatant is poured out. , collect the precipitate, and then extrude for dehydration. The filter membrane pore size of the extrusion dehydration is 50 microns, the extrusion pressure is 10MPa, and the dehydration extrusion time is 40 minutes. Finally, a collagen gel with a solid content of about 120 mg / liter is obtained. The collagen gel was poured into a mold, and freeze-dried for 24 hours to obtain a repair material product with certain shape characteristics. Then put the above product into 5.5% EDC solution for cross-linking for 12 hours and freeze-dry for 24 hours to obtain the final repair material product. The article has a compressive strength of about 20 MPa.

Embodiment 3

[0033] The selected concentration is 2 g / L of collagen mother liquor mixed with saturated magnesium chloride solution, stirring continuously, the collagen gradually precipitates from the collagen solution, after the above solution is centrifuged at 8000 rpm, the supernatant is poured, collected Precipitate, extrusion dehydration again, the filter membrane pore diameter of extrusion dehydration is 200 microns, extrusion pressure is 1MPa, and dehydration extrusion time is 15 minutes, finally obtains the collagen gel that solid content is about 40 mg / liter, the The collagen gel was poured into the mold and freeze-dried for 24 hours to obtain a repair material product with certain shape characteristics. Then put the above product into 5.5% EDC solution for cross-linking for 12 hours and freeze-dry for 24 hours to obtain the final repair material product. The article has a compressive strength of about 8 MPa.

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Abstract

The invention relates to a method for preparing a high-strength collagen tissue repair material product. The method obtains the final collagen product through the treatment steps of salting out, centrifugation, dehydration, crosslinking and drying of a collagen solution. Because the treatment method adopts the mode of improving the solid content of collagen in collagen gel and the crosslinking density in the collagen gel, the mechanical property of the final product is greatly improved. Simultaneously, because the adopted method does not influence the biological property of the collagen, the final collagen product still maintains good biological activity. The collagen product can be used for the repair treatment of articular cartilage, meniscus and skin, the construction of artificial blood vessels and nerve conduits, and the like.

Description

technical field [0001] The invention relates to a tissue repair substitute material, in particular to a preparation method of a high-strength collagen tissue repair material product. Background technique [0002] With the development of modern society, there are more and more tissue damage problems caused by various reasons. Such as skin injuries caused by burns, scalds, knife wounds, firearm injuries, etc., limb blood vessel and nerve injuries caused by traffic accidents, loss of surrounding tissues caused by tumor resection, and articular cartilage and meniscus damage caused by sports injuries and pathological injuries. damage etc. There are various reasons for the injury, and the final result is the damage of connective tissues such as articular cartilage, meniscus, skin, blood vessels and nerves. Small-area, small-scale, low-injury tissue damage can be repaired automatically by the human body through the mobilization of autologous tissues and cells. Regenerative docki...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/24A61L27/56A61L29/04
Inventor 吴刚王迎军
Owner SOUTH CHINA UNIV OF TECH
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