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Collagenolysis and regeneration method

A technology of collagen and collagen description, which is applied in the direction of animal/human protein, connective tissue peptide, single-component protein rayon, etc., can solve the problems of cumbersome extraction process, low extraction rate, and high cost, and achieve simplified preparation process and regeneration process Simple, easily recyclable effects

Inactive Publication Date: 2010-09-22
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that the whole extraction process of collagen is quite cumbersome, and the process will generate a lot of waste, and the extraction rate is also very low
At the same time, the method of freeze-drying requires special instruments, which is time-consuming and expensive

Method used

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  • Collagenolysis and regeneration method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Weigh 0.56g skin powder fiber and 5.03g ionic liquid 1-methyl-3-butylchloroimidazole into a beaker, and heat up to 100°C while magnetically stirring; skin powder fiber gradually dissolves in the ionic liquid for 1.5h Afterwards, the solution is evenly dispersed and turbid. Continue to stir, and the solution gradually becomes clear until the skin powder fibers are completely dissolved.

[0023] Take a small amount of the prepared skin powder fiber ionic liquid solution, spread it evenly on the glass slide, immerse the slide in deionized water and let it stand, and the skin collagen will regenerate into a film. The membrane was repeatedly soaked in deionized water, the ionic liquid was thoroughly washed off, and the membrane was naturally dried in a ventilated place at room temperature to obtain a regenerated collagen membrane.

Embodiment 2

[0025] Weigh 0.25g skin powder fiber and 3.00g ionic liquid 1-butyl-3-methylchloroimidazole, add 0.30g deionized water, heat to 120°C in an oil bath, and carry out magnetic stirring; The aqueous ionic liquid was gradually dissolved, and after 1 hour, the solution was in a uniformly dispersed turbid state. Continue stirring, and the solution gradually became clear until the skin powder fibers were completely dissolved.

[0026] Take a small amount of prepared dermal fiber ionic liquid solution with a syringe, inject the solution into acetone, and regenerate collagen into fibers. Repeatedly soaking the fibers in acetone, thoroughly washing away the ionic liquid, and drying the fibers naturally in a ventilated place at room temperature to obtain regenerated collagen fibers.

Embodiment 3

[0028] Scrape off the corium layer of the pickled goatskin, neutralize it, and fully dry it in a vacuum oven at room temperature to obtain corium fiber. Weigh 0.6g of dermis fibers and 5.03g of ionic liquid 1-butyl-3-methylimidazole bromide, heat to 85°C in an oil bath, and carry out magnetic stirring; the fibers gradually dissolve in the ionic liquid, and after 6h, The solution is in a uniformly dispersed turbid state. Continue stirring until the solution gradually becomes clear until the fibers are completely dissolved.

[0029] Take a small amount of prepared dermal fiber ionic liquid solution with a syringe, drop the solution into acetone, and regenerate the skin collagen into a micropowder. The micropowder is obtained after suction filtration, and the micropowder is naturally dried in a ventilated place at room temperature to obtain regenerated skin collagen powder.

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Abstract

The invention relates to a collagenolysis and regeneration method, aiming at providing a simple and convenient collagenolysis method; meanwhile, the invention further provides a method which leads the lysis collagen to regenerate membranes, fibers, microballoons or powdery products. In order to realize the purpose, the invention adopts the following technical proposal: in the collagenolysis method, ionic liquid is utilized as solvent for collagen lysis, and the ionic liquid is alkyl imidazoles, including 1-methyl-3-butyl halogenated imidazole, 1-butyl-3-methyl halogenated imidazole, 1-ethyl-3-methyl halogenated imidazole and 1-allyl-3-methyl halogenated imidazole or halide (3,3-dimethyl) diimidazole sulfoxide salt. In the collagen regeneration method, the collagenolysis method is utilized to prepare collagen / ionic liquid solution, and then coagulators are utilized to wash out the ionic liquid in the solution, and then the regeneration collagen is obtained. In the collagenolysis and regeneration method, the steps are simple, nontoxic and harmless, and have high safety, thereby establishing good basis for research and application and dissemination of the collagen products.

Description

technical field [0001] The present invention relates to a method for dissolving collagen (collagen), and a method for further utilizing water or other coagulation baths to regenerate the dissolved collagen into various forms. Background technique [0002] Collagen is one of the most abundant and widely distributed proteins in animals. It widely exists in connective tissue, skin, bones, tendons, viscera, interstitial cells, ligaments, blood vessels, sclera and other parts of animals. It accounts for the total amount of protein in animals. 25-30%, is the main structural protein in animals. As an excellent renewable natural polymer resource, collagen has excellent biocompatibility and degradability, and has been widely used in biomedical materials, leather chemistry and engineering, daily chemical industry, food industry, etc. Broad application prospects. In recent years, due to the shortage of petroleum resources, environmental degradation and the challenges of sustainable d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/11C08L89/00C08J3/12D01F4/00C07K14/78
Inventor 郑学晶汤克勇孟卓君
Owner ZHENGZHOU UNIV
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