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Method for extracting tetrodotoxin

A technology of tetrodotoxin and toxin, which is applied in the direction of organic chemistry, can solve the problems of complex process and lower TTX extraction yield, and achieve the effects of improving extraction yield, benefiting environmental protection, and avoiding phase reaction

Inactive Publication Date: 2003-06-25
HUATENG BIOLOGICAL ENG SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] In the above-mentioned process, in addition to the complicated process, factors such as phase conversion loss, high temperature damage to TTX, and activated carbon adsorption all greatly reduce the extraction rate of TTX, which has become a major obstacle to the progress of this technical field.

Method used

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Embodiment Construction

[0012] The method for extracting tetrodotoxin of the present invention mainly comprises the following steps:

[0013] 1. Firstly, fresh puffer fish viscera (such as liver and ovary) are minced, ground, and extracted with water, and then filtered through a filter press with a filter membrane pore size of 10-20 μm to obtain a clear liquid;

[0014] 2. Heat the above clear liquid at a temperature of 60°C to 100°C for a heating time of 10 minutes to 60 minutes, remove protein and grease after cooling, and obtain a concentrated clear liquid;

[0015] 3. Continuous filtration: The above-mentioned concentrated clear liquid is continuously filtered in the following order respectively. First, the concentrated clear liquid is filtered in a filter with a filter membrane pore size of 1 μm to 5 μm to obtain a new clear liquid. The above-mentioned filter membrane can be Cellulose (CA) membrane, polysulfone (PA) membrane, polypropylene (PP) membrane, polytetrafluoroethylene (PTFE) membrane, ...

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Abstract

The present invention relates to matter extraction from animal material and is the extraction process of tetrodotoxin. The present invention provides the technological method of raising yield of tetrodotoxin. The technological process includes the steps of: cutting internal organs of globefish, grinding into slurry, press filtering, heating to eliminate protein and oil, filtering to obtain clear liquid, concentration, elution of crystal inchromatographic column to obtain pure tetrodotoxin product.

Description

Technical field: [0001] The invention relates to a substance extracted from animal materials, especially a method for extracting tetrodotoxin from puffer fish. Background technique: [0002] Tetrodotoxin (TTX) is a marine biotoxin that exists in puffer fish, salamanders, snails, crabs, octopus, etc. The main source is the internal organs of puffer fish. The molecular formula of TTX is: C 11 h 17 o 8 N 3 , molecular weight: 319. Highly toxic, the LD of mice is 10μg / kg. [0003] TTX is a very useful tool drug for neurophysiological research because it can block the channel of sodium ions in cells in a very small amount, but has no effect on potassium ion channels and has a high degree of specificity. [0004] It has been proved clinically that TTX is a good sedative, antispasmodic, and analgesic, especially for cancer and other malignant pains. Compared with traditional analgesics such as pethidine and morphine, although the response time is slightly longer , but its ana...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D491/20
Inventor 梁映华
Owner HUATENG BIOLOGICAL ENG SHANGHAI
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