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Method for extracting fish collagen

A technology of fish collagen and extraction method, which is applied in the field of fish collagen extraction, can solve the problems of long operation time, pollution, low fat content, etc., and achieve the effect of less fat content, good water solubility and high purity

Active Publication Date: 2017-07-21
GUANGDONG TIMES FOOD & LIFE HEALTH RES CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is the problem of long operating time and serious pollution caused by the need to use more organic solvents in the lipid and protein separation process of fish collagen; the present invention adopts a method that does not use organic solvents The fish collagen extraction process realizes the removal and separation of fat. The main technical concept is: firstly, through the grinding and homogenization under the action of ultrasound, the fish skin tissue cells are fully broken, so that the fat and protein can be separated in the subsequent alkali treatment and enzyme treatment. More can be removed during the process, and a crude extract with low fat content can be obtained. Next, the crude extract solution is passed through a lipophilic modified resin bed to achieve the purpose of adsorbing low-content lipids.

Method used

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  • Method for extracting fish collagen

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Step 1, tissue crushing: After thawing crucian carp skin, add water to make the weight ratio of fish skin to water 10:0.2, crush and grind the mixture, and apply ultrasonic action while crushing and grinding to obtain slurry ;

[0031] The second step is to remove salt-soluble impurities: mix the slurry with 4wt% NaCl solution, the weight ratio of slurry and NaCl solution is 1:5, stir evenly and soak for 10 hours, then centrifuge, remove the supernatant, and get first precipitation;

[0032]Step 3, remove lipids and alkali-soluble impurities: mix the first precipitate with 2wt% NaOH solution, the weight ratio of the first precipitate and alkali solution is 1:7, stir well and soak for 20 hours, then centrifuge to remove supernatant to obtain the second precipitate;

[0033] Step 4, remove acid-soluble impurities: mix the second precipitate with 5wt% citric acid solution, the weight ratio of the second precipitate and acid solution is 1:4, stir well and soak for 5 hours...

Embodiment 2

[0039] Step 1, tissue crushing: after the crucian carp skin is thawed, add water so that the weight ratio of fish skin to water is 10:1, crush and grind the mixture, and apply ultrasound while crushing and grinding to obtain a slurry ;

[0040] Step 2, remove salt-soluble impurities: mix the slurry with 10wt% NaCl solution, the weight ratio of slurry and NaCl solution is 1: 10, soak after stirring evenly, the soaking time is 30h, then go through centrifugation, remove the supernatant, and get first precipitation;

[0041] Step 3, remove lipids and alkali-soluble impurities: mix the first precipitate with 5wt% NaOH solution, the weight ratio of the first precipitate and alkali solution is 1: 12, stir well and soak for 35 hours, then centrifuge to remove supernatant to obtain the second precipitate;

[0042] Step 4, remove acid-soluble impurities: mix the second precipitate with 12wt% citric acid solution, the weight ratio of the second precipitate and acid solution is 1:8, st...

Embodiment 3

[0048] Step 1, tissue crushing: After thawing crucian carp skin, add water to make the weight ratio of fish skin to water 10:0.6, crush and grind the mixture, and apply ultrasonic action while crushing and grinding to obtain slurry ;

[0049] The second step is to remove salt-soluble impurities: mix the slurry with 6wt% NaCl solution, the weight ratio of slurry and NaCl solution is 1:7, stir evenly and soak for 20 hours, then centrifuge, remove the supernatant, and get first precipitation;

[0050] Step 3, remove lipids and alkali-soluble impurities: mix the first precipitate with 4wt% NaOH solution, the weight ratio of the first precipitate and alkali solution is 1:11, stir well and soak for 26 hours, then centrifuge to remove supernatant to obtain the second precipitate;

[0051] Step 4, remove acid-soluble impurities: mix the second precipitate with 5-12wt% citric acid solution, the weight ratio of the second precipitate to the acid solution is 1:6, stir well and then soa...

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Abstract

The invention relates to a method for extracting fish collagen, and belongs to the technical field of natural protein extraction. Through adoption of a fish collagen extracting process in which an organic solvent is not used, removal and separation of fat are achieved. The invention adopts the technical concept as follows: firstly, through grinding and homogenizing under an ultrasonic action, tissue cells of fish skin are completely broken, and during subsequent alkali treatment and enzymatic treatment, fat and protein can be more removed, so that a crude extract with low fat content can be obtained; then, a crude extract solution is enabled to pass through a resin bed obtained after lipophilic modification, so that the purpose of adsorbing low-content lipids is achieved. The prepared fish collagen has the advantages of high purity, low fat content and good water solubility.

Description

technical field [0001] The invention relates to a method for extracting fish collagen, belonging to the technical field of natural protein extraction. Background technique [0002] Collagen is a white, opaque, unbranched, fibrous protein that plays the role of binding tissue in animal cells. Collagen is rich in 18 kinds of amino acids except tryptophan and cysteine, among which there are 7 kinds of amino acids necessary to maintain human growth. Glycine in collagen accounts for 30%, proline and hydroxyproline account for about 25%, which is the highest content in various proteins, and the content of alanine and glutamic acid is also relatively high. At the same time, it also contains hydroxyproline and pyroglutamic acid, which are rare in general proteins, and hydroxylysine, which hardly exists in other proteins. Therefore, collagen is very nutritious. Fish collagen is mainly extracted from tilapia, cod fresh skin and fish scales through bio-enzyme directional shearing te...

Claims

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Application Information

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IPC IPC(8): C07K14/78C07K1/22
CPCC07K14/78
Inventor 张军志
Owner GUANGDONG TIMES FOOD & LIFE HEALTH RES CO LTD
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