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Preparation method and use of fish bait for resisting marine pathogenic vibrios

A technology of pathogenic Vibrio and bait, applied in the field of aquatic feed preparation, can solve problems such as economic loss, emergence of drug-resistant strains, threat to marine natural biological resources, etc., and achieve a reliable model, fast extraction method, and high industrialization value. Effect

Pending Publication Date: 2021-10-26
JIANGSU OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These pathogenic bacteria not only cause huge economic losses to the mariculture industry, but also threaten marine natural biological resources.
At present, chemical drug control methods are mostly used for marine pathogenic bacteria, mainly including penicillin, norfloxacin, sulfamethoxazole, etc., but long-term single use of a drug will produce resistance, and drug-resistant strains will appear

Method used

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  • Preparation method and use of fish bait for resisting marine pathogenic vibrios
  • Preparation method and use of fish bait for resisting marine pathogenic vibrios
  • Preparation method and use of fish bait for resisting marine pathogenic vibrios

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1, Extraction Process Optimization Method for Phenolic Acids in Cornus officinalis

[0032] 1.1 Preparation of sample extract

[0033] Weigh 1.0g of Cornus officinalis powder, add solvent ethanol and mix evenly, control ultrasonic power to 225w, ultrasonic temperature to 50°C, and extract phenolic acid compounds at different ethanol concentrations, solid-liquid ratios, ultrasonic time, reflux time, and reflux temperatures. The extract is obtained by suction filtration, extracted twice, centrifuged and the solvent is recovered to obtain the extract. Dissolve the extract with a small amount of solvent and transfer it to a 10mL volumetric flask, and constant volume to obtain a 0.1g / mL sample extract.

[0034] 1.2 Determination of phenolic acid compounds in Cornus officinalis

[0035] 1.2.1 Drawing of gallic acid standard curve

[0036] Accurately weigh 0.5 mg of gallic acid, dissolve it in 10 mL of distilled water, and prepare a 0.05 mg / mL gallic acid solution. ...

Embodiment 2

[0045] Embodiment 2, fish bait preparation method and antibacterial experiment thereof

[0046] 2.1 Fish bait preparation method

[0047] After heating and steaming 25g of glutinous rice, add 40% ethanol according to the ratio of material to liquid (1:0.2g / mL) while it is hot, mix well and cool to 40°C, then add rapeseed cake powder in proportion (1:2g / g) , Cornus officinalis extract (1:0.1g / mL), mix well, chop and dry.

[0048] 2.2 The inhibitory effect of fish bait on Vibrio harveyi at the living level of perch

[0049] Thirty perch (body length 8-10cm) were randomly divided into two groups, namely experimental group 1, experimental group 2 and control group. Experimental group 1 was fed with fish bait prepared from Cornus officinalis, experimental group 2 was fed with fish bait prepared from Cornus officinalis extracted by optimal technology, and the control group was fed with commercially available allicin aquatic fish medicine. First, the perch were cultured in the rec...

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Abstract

The invention relates to a preparation method of a fish bait for resisting marine pathogenic vibrios. The preparation method comprises the following steps: heating and steaming glutinous rice, adding ethanol with a mass concentration of 30%-70% while the glutinous rice is hot, and controlling a mass volume ratio of the glutinous rice to the ethanol to be (1:0.01)-(1:0.05) g / mL; uniformly mixing and cooling, sequentially adding rape seed cake powder and a fructus corni extracting solution in proportion, and uniformly stirring, chopping and drying to obtain the fish bait; wherein a mass ratio of the glutinous rice to the rape seed cake powder is 1:1 to 1:10, and a mass volume ratio of the glutinous rice to the fructus corni extracting solution is 1:0.1 to 1:10 g / mL. The fructus corni is taken as a raw material, a technology for extracting a fructus corni phenolic acid compound is optimized by adopting a Box-Behnken response surface method, the yield of the fructus corni phenolic acid compound is improved, and an activity of the extract in resisting marine pathogenic bacteria is improved. The prepared fish bait is environmentally friendly, safe, reliable and capable of effectively resisting marine pathogenic bacteria, and has high industrialization value.

Description

technical field [0001] The invention relates to the technical field of aquatic feed preparation, in particular to a method for preparing fish bait resistant to marine pathogenic Vibrio, and also relates to the application of the product prepared by the method. Background technique [0002] Cornus officinalis, also known as jujube, mouse arrow, chicken foot, real jujube, meat jujube, medicine jujube, etc., is the dry and mature pulp of Cornus officinalis, a plant of the genus Cornus in the family Cornaceae. It is mainly produced in Shaanxi, Shanxi, and Shandong in China. , Gansu, Jiangsu, Anhui, Zhejiang and other places. It is sour and astringent in taste and slightly warm in nature. It is an astringent and strong medicine. It has the effect of nourishing the liver and kidney, astringent and solidifying. It is used to treat various diseases such as high blood pressure, kidney deficiency and cold, dizziness, tinnitus, and soreness of the waist and knees. Modern studies have ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/80A23K10/30A23K10/37A23K20/105A61K36/40A61P31/04
CPCA23K50/80A23K10/30A23K10/37A23K20/105A61K36/40A61P31/04Y02P60/87Y02A40/818
Inventor 刘玮炜邵仲柏蒋凯俊曹联攻陈超吴玉宇苏子钦王有宪刘书豪
Owner JIANGSU OCEAN UNIV
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