Process for hydrolyzing fish processing leftover using emzyme preparation
A technology for leftovers and enzyme preparations, applied in the field of biological enzyme preparation processing technology, can solve the problems of environmental pollution, resource waste, underutilization, etc., and achieve the effect of tapping resource potential and increasing added value.
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Embodiment 1
[0014] Embodiment 1: A kind of technology that utilizes enzyme preparation to hydrolyze fish processing leftovers, realize through following steps:
[0015] (1) Take fish fillet as raw material, add water at a ratio of 1:1 to adjust the concentration of the substrate, and beat;
[0016] (2) Enzyme inactivation at high temperature: heat the slurry to 85°C for 10 minutes;
[0017] (3) Cool down to 35°C, adjust the pH value to 8.5, and keep warm for 50 minutes;
[0018] (4) enzymatic hydrolysis: add alkaline protease to react, the addition of alkaline protease is 0.2% of raw material weight, and keep warm for 4 hours;
[0019] (5) Enzyme inactivation: the temperature is 90° C., and the time is 10 minutes; a hydrolyzate is obtained.
[0020] The hydrolyzate prepared by this method is a clarified hydrolyzate, which has the fresh flavor of fish and is completely separated from the fish bones.
Embodiment 2
[0021] Embodiment 2: A kind of technology that utilizes enzyme preparation to hydrolyze fish processing leftovers is realized through the following steps:
[0022] (1) Use the viscera of fish as raw material, add water at 1:1 to adjust the concentration of the substrate, and beat;
[0023] (2) Enzyme inactivation at high temperature: heat the slurry to 85°C for 10 minutes;
[0024] (3) Cool down to 35°C, adjust the pH value to 8.5, and keep warm for 50 minutes;
[0025] (4) Enzymolysis: adding a mixture of neutral protease and trypsin for reaction, the weight ratio of neutral protease and trypsin is 4:1, the amount added is 0.5% of the weight of the raw material, and incubated for 4 hours;
[0026] (5) Enzyme inactivation: the temperature is 90° C., and the time is 10 minutes; a hydrolyzate is obtained.
[0027] The hydrolyzate prepared by this method is white jelly and has the fresh flavor of fish.
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