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Process for preparing micro-molecular fish scale collagen peptides

The invention relates to a fish scale collagen and a preparation process technology, which is applied in the field of preparation of small molecule fish scale collagen peptides, and can solve the problems of affecting the enzymatic activity, waste, low yield and the like, so as to facilitate large-scale amplification, save costs, and reduce the use of enzymes. amount of effect

Active Publication Date: 2011-08-17
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The molecular weight of the product prepared by this process is relatively concentrated, and the added value of the product is high. However, in addition to collagen, fish scales also contain a large amount of impurities such as inorganic salts and fats. Too low to meet industrial mass production
Compared with enzymatic extraction, acid extraction will relatively reduce the cost, but the product obtained by single acid extraction has a wide molecular weight distribution and poor uniformity
The combination of acid method and enzyme method can increase the yield, and the molecular weight of the prepared product is relatively concentrated, but the yield is still very low, and most of the collagen peptides of fish scales cannot be extracted, resulting in waste

Method used

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  • Process for preparing micro-molecular fish scale collagen peptides
  • Process for preparing micro-molecular fish scale collagen peptides

Examples

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Effect test

example 1

[0032] 1. Use high-quality fish scales as raw materials, and the selected fish scales can be marine fish scales or freshwater fish scales. Get 500kg of fish scales and drop into the stirring washer, add 5000kg of water, wash 3 times, and remove particle impurities. Put the washed fish scales into the reaction kettle, add 5000kg of sodium bicarbonate solution with a mass volume concentration of 4%, stir for 12 hours, remove the sticky substances on the surface of the fish scales, the fat and other impurities of the fish scales, and wash them with water.

[0033] 2. Put the cleaned fish scales in the reaction kettle, add 5000kg of water, add sulfuric acid to adjust the pH value and control the reaction pH to 2, the reaction temperature is 90°C, react for 6 hours, and end the reaction to obtain macromolecular fish scale collagen peptides , the extraction yield was 97%.

[0034] 3. Separate the above-mentioned macromolecular fish scale collagen peptide solution with a 13000rpm co...

example 2

[0038] 1. Use high-quality fish scales as raw materials, and the selected fish scales can be marine fish scales or freshwater fish scales. Get 100kg of fish scales and drop into the stirring washer, add 1000kg of water, wash 3 times, and get rid of particle impurities. Put the washed fish scales into the reaction kettle, add 500 kg of 1% sodium hydroxide solution, stir for 11 hours, remove sticky substances on the surface of the fish scales, fat and other impurities on the fish scales, and wash them with water.

[0039] 2. Put the cleaned fish scales in a reaction kettle, add 1000kg of water, add sulfuric acid to adjust the pH value and control the reaction pH to 2, the reaction temperature is 100°C, react for 5 hours, and end the reaction to obtain macromolecular fish scale collagen peptides. The extraction yield was 98%.

[0040] 3. Separate the above-mentioned macromolecular fish scale collagen peptide solution with a 15000rpm continuous flow centrifuge to remove granular ...

example 3

[0044] 1. Use high-quality fish scales as raw materials, and the selected fish scales can be marine fish scales or freshwater fish scales. Get 50kg of fish scales and drop into the stirring washer, add 500kg of water, wash 3 times, and remove particle impurities. Put the washed fish scales into the reaction kettle, add 100 kg of sodium bicarbonate solution with a mass volume concentration of 3%, stir for 10 hours, remove sticky substances on the surface of the fish scales, fat and other impurities on the fish scales, and wash them with water.

[0045] 2. Put the cleaned fish scales in a reaction kettle, add 500kg of water, add sulfuric acid to adjust the pH value and control the reaction pH to 3, the reaction temperature is 70°C, react for 8 hours, and end the reaction to obtain macromolecular fish scale collagen peptides. The extraction yield was 96%.

[0046] 3. Separate the above-mentioned macromolecular fish scale collagen peptide solution with a continuous flow centrifug...

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Abstract

The invention discloses a process for preparing micro-molecular fish scale collagen peptides and belongs to the technical field of biological extraction. The process comprises the following steps of: 1, pretreating fish scales, and removing impurities and fat on the surfaces of fish scales; 2, extracting fish scale collagen peptides by using a high-temperature sulfuric acid extraction method, wherein the extraction yield is over 95 percent; 3, purifying the extracting solution by adopting the process technology combining centrifugal separation and membrane separation to obtain macro-molecularfish scale collagen peptides of which the purity is over 90 percent; 4, hydrolyzing the macro-molecular fish scale collagen peptides by using protease, and performing enzymic inactivation to obtain the solution of micro-molecular fish scale collagen peptides of which the purity is more than 90 percent and the relative molecular weight is less than 1.5kD; and 5, spray-drying and quickly drying thesolution of collagen peptides to obtain the finished product of micro-molecular fish scale collagen peptides. The micro-molecular fish scale collagen peptides prepared by the process have the advantages of low cost, high yield, high purity and low molecular weight; moreover, in the process, the fish scales discarded in fish processing are fully utilized, and the wastes are changed into the valuables.

Description

technical field [0001] The invention relates to a preparation process of small molecule fish scale collagen peptide, which belongs to the technical field of biological extraction. Background technique [0002] Collagen peptides are widely used in food, cosmetics, and health care products industries. In the past, the raw materials for the preparation of collagen peptides mainly came from the bones and skins of terrestrial animals. In recent years, due to the occurrence of mad cow disease, foot-and-mouth disease, bird flu and other epidemics, the source of collagen peptide products is limited. At the same time, considering the safety and cost of raw materials, fish scales, which are waste from fish processing, have become important raw materials for the preparation of collagen peptides. [0003] Fish scale collagen peptides have been widely studied in Japan, the United States, Norway and other countries in the 1990s. After entering the 21st century, domestic research on the e...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/14
Inventor 陈俊德易瑞灶陈晖陈思谨张怡评孙继鹏郭洪辉刘颖
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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