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Application of jatrorrhizine hydrochloride in preparation of drug for preventing and treating influenza

A technology of jatrorrhizine hydrochloride and medicine is applied in the application field of jatrorrhizine hydrochloride in the preparation of influenza virus-inhibiting medicines, and can solve the problems of unclear action principle, unknown effective ingredients, and hindered development of traditional Chinese medicines.

Inactive Publication Date: 2015-01-07
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in foreign countries, the development of Chinese medicine is hindered abroad due to the unknown active ingredients and mechanism of action in traditional Chinese medicine.

Method used

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  • Application of jatrorrhizine hydrochloride in preparation of drug for preventing and treating influenza
  • Application of jatrorrhizine hydrochloride in preparation of drug for preventing and treating influenza
  • Application of jatrorrhizine hydrochloride in preparation of drug for preventing and treating influenza

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] We used allantoic fluid containing influenza virus NA (passaged through chicken embryos, collected allantoic fluid, and stored at -80°C) to prepare in pH 6.5 MES buffer (32.5mM). A mixture of 41 μL jatrorrhizine hydrochloride was added to the reaction buffer, placed in a 37°C incubator for 30 minutes, then 50 μL of 20 μM MU-NANA substrate buffer was added, and detected on a microplate reader with an excitation wavelength of 360 nm. The emission wavelength is 450nm, and the detection time is 8min. The detection result is that jatrorrhizine hydrochloride is to H1N1, H3N2 and B type influenza virus NA half constant concentration (IC 50 ) were 120.40±20.07 μmol / L, 193.40±21.70 μmol / L and 178.05±8.57 μmol / L, respectively.

Embodiment 2

[0015] In order to detect the anti-influenza effect of jatrorrhizine hydrochloride at the cellular level, we experimented with two different treatments: first, the jatrorrhizine hydrochloride was diluted into different concentrations according to the concentration gradient, and the above solution was mixed with 100TCID 50 Equal volumes of different subtypes of influenza viruses (H1N1 and H3N2) were incubated for 2 hours in a total volume of 200 μL. Then add the mixture to MDCK cells and incubate for 2h; II add 100TCID 50 Different subtypes of influenza viruses (H1N1 and H3N2) were incubated in MDCK cells for 2 hours, and then incubated with different concentrations of jatrorrhizine hydrochloride for 2 hours. The maintenance solution (10 μg / ml trypsin in DMEM) was used for both different treatment dilutions. After 2 hours, the above-mentioned maintenance solution was changed once to continue culturing for 24 hours. After 24 hours, the maintenance solution containing 2% serum ...

Embodiment 3

[0018] In order to detect the protective effect of jatrorrhizine hydrochloride in the mouse influenza model, we used 50μL 10LD 50 Intranasally infected mice with FM1 virus. The experimental mice were randomly divided into 6 groups, namely the NC group; 10LD 50 group; 10LD50+50mg / kg Tamiflu group; jatrorrhizine hydrochloride low, medium and high dose groups: 10LD 50 +50, 100, 200mg / kg jatrorrhizine hydrochloride. There were 10 mice in each group, and the drug was given 1 day before the virus challenge, and the drug was administered by gavage, twice a day, for 7 consecutive days, and each mouse was gavaged with 0.2ml. The survival status and body weight changes of the mice were recorded every day. Then at day 15, the mice were sacrificed, the lung weight was recorded, the lung tissue was ground and added with PBS, and then the NA activity was measured.

[0019] The result is as Figure 4 and Figure 5 As shown, it shows that jatrorrhizine hydrochloride can inhibit the weig...

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Abstract

The invention discloses application of jatrorrhizine hydrochloride in preparation of a drug for preventing and treating influenza, and belongs to the field of traditional Chinese medicine application. The application is implemented by the following technical scheme: firstly incubating influenza virus neuraminidase liquid from different subtypes and jatrorrhizine hydrochloride in an incubator at 37 DEG C for 30 minutes, adding a fluorogenic substrate buffer solution to the incubator to be detected, finding out the situation that jatrorrhizine hydrochloride can play an effective role on H1N1, H3N2 and influenza virus B, detecting anti-influenza action of jatrorrhizine hydrochloride on a cellular level, finding out the situation that jatrorrhizine hydrochloride can treat the cells infected H1N1 and H3N2, and at last detecting the influence of jatrorrhizine hydrochloride on a mouse influenza model. The study shows that when jatrorrhizine hydrochloride is added, the mouse weight reducing degree of the influenza infected mice can be obviously inhibited; jatrorrhizine hydrochloride can enable the mice to gradually recover to the health level and obviously lower the lung index of the influenza infected mice.

Description

technical field [0001] The invention belongs to the field of application of traditional Chinese medicines, and in particular relates to the application of jatrorrhizine hydrochloride in the preparation of drugs for inhibiting influenza virus. Background technique [0002] Influenza (hereinafter referred to as influenza) is an acute viral respiratory infectious disease caused by RNA viruses of the family Myxoviridae. It is highly contagious, widespread, and has a high incidence rate. About 5% to 15% of the world is infected every year. Myxoviridae is divided into 5 genera: Influenza A virus, Influenza B virus, Influenza virus C, Infectious salmon anemia virus and Togotovirus. At present, influenza A virus is the most common and most likely to cause a world pandemic and regional epidemic. [0003] Influenza A virus RNA consists of 8 segments, encoding 10 proteins; the sixth segment encodes neuraminidase (NA) protein. NA is one of the two proteins present on the envelope of i...

Claims

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Application Information

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IPC IPC(8): A61K31/4375A61P31/16
CPCA61K31/4375
Inventor 麻彤辉房学迅于淼于大海田莉王烨杨吉雨王溪竹梁伟国
Owner JILIN UNIV
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