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Detecting method for 5 flavones and 4 alkaloids in blood plasma

A technique of plasma and legal quantification, applied in the fields of chemistry and medicine

Inactive Publication Date: 2014-02-12
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Wogonin, chrysin, scutellarin A, naringenin, hesperetin, berberine, coptisine, yaojingine and palmatine are the nine active ingredients in Tangminling, but there is no A method that can simultaneously detect these 9 components in plasma samples

Method used

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  • Detecting method for 5 flavones and 4 alkaloids in blood plasma
  • Detecting method for 5 flavones and 4 alkaloids in blood plasma
  • Detecting method for 5 flavones and 4 alkaloids in blood plasma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0138] LC-MS / MS conditions

[0139] Column: ZORBAX SB-C 18 (150×2.1mm, 5μm, Agilent)

[0140] Pre-column: Security Guard-C 18 (4.0mm×3.0mm i.d, 5μm, Phenomenex)

[0141] Mobile phase: water (containing 0.1% formic acid)-acetonitrile (30:70, v / v)

[0142] Column temperature: 25°C

[0143] Flow rate: 200μL·min -1

[0144] Ion source: electrospray ionization source (ESI)

[0145] Ionization mode: positive ion mode

[0146] MS / MS: Selected Reaction Monitoring (SRM). The ion reactions used for quantitative analysis were m / z 255→153 (chrysin), m / z 285→270 (wogonin and scutellarin A), m / z 273→153 (naringenin), m / z z 303→153 (hesperetin), m / z 255→199 (internal standard, daidzein), m / z 320→292 (coptisine), m / z 338→322 (drugine), m / z z352→336 (palmatine), m / z 336→320 (berberine), m / z 356→192 (internal standard, tetrahydropalmatine).

[0147] Mass spectrometer parameters: spray voltage: 4000V; ion source voltage: 10eV; capillary temperature: 350°C; sheath gas: N 2 (35psi); Au...

Embodiment 2

[0156] LC-MS / MS conditions

[0157] Column: XBridge-C 18 (150×2.1mm, 5μm, Waters)

[0158] Pre-column: Security Guard-C 18 (4.0mm×3.0mm i.d, 5μm, Agilent)

[0159] Mobile phase: water (containing 0.01% formic acid)-acetonitrile (60:40, v / v)

[0160] Column temperature: 25°C

[0161] Flow rate: 200μL·min -1

[0162] Ion source: electrospray ionization source (ESI)

[0163] Ionization mode: positive ion mode

[0164]MS / MS: Selected Reaction Monitoring (SRM). The ion reactions used for quantitative analysis were m / z 255→153 (chrysin), m / z 285→270 (wogonin and scutellarin A), m / z 273→153 (naringenin), m / z z 303→153 (hesperetin), m / z 255→199 (internal standard, daidzein), m / z 320→292 (coptisine), m / z 338→322 (drugine), m / z z352→336 (palmatine), m / z 336→320 (berberine), m / z 356→192 (internal standard, tetrahydropalmatine).

[0165] Mass spectrometer parameters: spray voltage: 4000V; ion source voltage: 10eV; capillary temperature: 350°C; sheath gas: N 2 (35psi); Auxiliar...

Embodiment 3

[0172] LC-MS / MS conditions

[0173] Column: WondaSil-C 18 (150×2.1mm, 5μm, Shimadzu)

[0174] Pre-column: Security Guard-C 18 (4.0mm×3.0mm i.d, 5μm, Phenomenex)

[0175] Mobile phase: water (containing 0.5% formic acid)-acetonitrile (30:70, v / v)

[0176] Column temperature: 25°C

[0177] Flow rate: 200μL·min -1

[0178] Ion source: electrospray ionization source (ESI)

[0179] Ionization mode: positive ion mode

[0180] MS / MS: Selected Reaction Monitoring (SRM). The ion reactions used for quantitative analysis were m / z 255→153 (chrysin), m / z 285→270 (wogonin and scutellarin A), m / z 273→153 (naringenin), m / z z 303→153 (hesperetin), m / z 255→199 (internal standard, daidzein), m / z 320→292 (coptisine), m / z 338→322 (drugine), m / z z352→336 (palmatine), m / z 336→320 (berberine), m / z 356→192 (internal standard, tetrahydropalmatine).

[0181] Mass spectrometer parameters: spray voltage: 4000V; ion source voltage: 10eV; capillary temperature: 350°C; sheath gas: N 2 (35psi); Au...

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Abstract

The invention discloses an analysis method for detecting wogonin, chrysin, oroxylin A, naringenin, hesperetin, berberine, coptisine, jatrorrhizine and palmatine in blood plasma, and applications of the method in pharmacokinetics. The analysis method has good specificity, high precision and accuracy, and wide linear range, and can be used for pharmacokinetic detection in vivo of traditional Chinese medicine compositions.

Description

technical field [0001] The present invention relates to the fields of medicine and chemistry, in particular to liquid chromatography tandem mass spectrometry for the quantitative detection of wogonin, chrysin, melaleurin A, naringenin, hesperetin, berberine, coptisine, drug Follow the method of alkali and palmatine. Background technique [0002] Mass spectrometry is an analysis method based on the determination of molecular weight. In recent years, its combination with separation mechanisms such as high performance liquid chromatography (HPLC) or capillary electrophoresis (CE) has not only improved its ability to resist interference from impurities and saved analysis time. time, and greatly improved the sensitivity of the assay. The development of soft ionization techniques, such as electrospray ionization (ESI), has expanded the range of molecular weight detection. In addition, electrospray ionization technology can be carried out under atmospheric pressure, which is conv...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 佟玲周水平朱永宏马晓慧靳元鹏
Owner TIANJIN TASLY PHARMA CO LTD
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