Medicine root berberrubine derived from amur cork-tree processed product and separation preparation method and application thereof
A technology of jatrorrhizine and its processed products, which is applied in the field of jatrorrhizine and its separation and preparation, which can solve the problems of changes in drug properties and differences in pharmacological effects, and achieve the effects of good phagocytosis, simple operation, and strong immune effect
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Embodiment 1
[0021] 1. Instruments and materials.
[0022] 1.1 Instruments: semi-preparative liquid chromatograph (Shimadzu LC-10AT); METTLERAE240 1 / 100,000 analytical balance (Switzerland METTLER); FA1004B electronic balance (Shanghai Precision Scientific Instrument Co., Ltd.); YP5102 electronic balance (Shanghai Guangzheng Medical Instrument Co., Ltd.); KQ-250DB CNC ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); nitrogen blowing instrument (Tianjin Hengao Technology Development Co., Ltd.); 101 electric blast drying oven (Beijing Yongguangming Medical Instrument Factory); CHRIST Vacuum Freeze Dryer (Germany); CAMAGLinomat-5 Semi-automatic Spotting Apparatus (Switzerland).
[0023] 1.2 Reagents: Phellodendron Phellodendri was purchased from Sichuan Medicinal Materials Company; jatrorrhizine hydrochloride reference substance was purchased from Sichuan Weikeqi Biotechnology Co., Ltd. (batch number 130218); Determination, purity > 98%; TLC silica gel plate (Qingdao Ocean Chemi...
Embodiment 2
[0059] This embodiment provides a method for the separation and preparation of jatorrhizine derived from cortex cortex processed products, including the following steps.
[0060] Step 1. Simulate processing of the jatrorrhizine reference substance, which is an index component in Cortex Phellodendri, accurately weigh 5 mg of jatrorrhizine hydrochloride reference substance, put it in an evaporating dish, bake it at 160°C for 15 minutes, take it out and let it cool, and add chromatographic grade Methanol 5mL was filtered through a microporous membrane (0.45 μm) to obtain the baked jatrorrhizine reference solution for the preparation of new components.
[0061] Step 2. Separation and preparation of new components of baked products of jatrorrhizine monomer.
[0062] Preparative liquid chromatography conditions: mobile phase A is water, of which 100ml water contains 0.4ml diethylamine, B is methanol; mobile phase flow rate: the mobile phase ratio of A and B is (70:30); 18 The silic...
Embodiment 3
[0065] This embodiment provides a method for the separation and preparation of jatorrhizine derived from cortex cortex processed products, including the following steps.
[0066] Step 1. Simulate the preparation of the jatrorrhizine reference substance, an index component in Cortex Phellodendri, accurately weigh 5 mg of jatrorrhizine hydrochloride reference substance, put it in an evaporating dish, bake it at 180°C for 25 minutes, take it out and let it cool, and add chromatographic grade Methanol 5mL was filtered through a microporous membrane (0.45 μm) to obtain the baked jatrorrhizine reference solution for the preparation of new components.
[0067] Step 2. Separation and preparation of new components of baked products of jatrorrhizine monomer.
[0068] Preparative liquid chromatography conditions: mobile phase A is water, of which 100ml water contains 0.4ml diethylamine, B is methanol; mobile phase flow rate: the mobile phase ratio of A and B is (90:10); 18 The silica ge...
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