Method for screening control enzyme of microalgae adipopexis

Abstract

The invention discloses a method for screening control enzyme of microalgae adipopexis. The method comprises the steps of (1) establishing a salt environment under the manual simulation of a laboratory, and culturing salt-sensitive chlorella and salt-resistant chlorella; (2) analyzing fluorescence difference gel electrophoresis proteomics of the whole protein of algae cells; and (3) screening and identifying key control enzyme of the microalgae adipopexis. The method disclosed by the invention has the advantages that due to the same key control enzyme for inducing grease synthesis in different algae plants by adopting the salt-resistant chlorella and the salt-sensitive chlorella as screening objects under the salt environment, the effective control for anabolism of microalgae grease with different energies and the construction of high-yield grease gene engineering microalgae are excepted to be realized; the error between gel and gel in the traditional two-dimensional electrophoresis (silver staining and coomassie-brilliant-blue staining) is eliminated, and the accuracy and the repeatability are improved; and simultaneously, an established general technology system for discovering and identifying the key control enzyme of the grease synthesis can be applied to the fields of metabonomics analysis of animals, plants, microorganisms and fungus in a radiation manner.

Description

technical field [0001] The invention belongs to the field of microalgae biochemical analysis, and in particular relates to a method for screening microalgae lipid accumulation regulating enzymes. Background technique [0002] Microalgae is one of the potential raw materials for biodiesel. The paradox is that many energy microalgae, such as brown algae, green algae, diatoms, and salina, have poor oil accumulation efficiency, which makes rapid oil production impossible. Microalgae biodiesel has therefore become a "noble" energy source. How to increase the oil yield of microalgae has always been a major problem in the industrialization of microalgae biodiesel. At present, the culture modes for obtaining lipid-accumulating microalgae mainly include the culture mode mediated by the abiotic environment and the "multi-stage" culture mode coupled with extreme environmental stress. [0003] The culture mode mediated by abiotic environment can induce high-efficiency oil accumulation...

AI Technical Summary

Problems solved by technology

At the same time, the traditional method for screening lipid accumulation markers is "one algae strain, two contrasting conditions", that is, by applying two extreme environmental stresses to the same algae strain (such as: high-salt stress and no-salt stress, Nitrogen abundance and nitrogen starvation) to discover and identify lipid metabolism regulatory points, the lipid metabolism regulatory enzymes selected by this method are not universal and instructive due to differences in algae species

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