Butyric acid bacterium fermentation culture medium and preparation method thereof and butyric acid bacterium culture fermentation method

A fermentation medium and butyric acid bacteria technology, applied in the field of bioengineering, can solve the problems of poor bacterial growth and stability, complex composition, long fermentation time, etc. Effect

Active Publication Date: 2014-05-21
TIANJIN SHENGJI GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the fermentation medium of probiotic butyric acid bacteria, the nitrogen source commonly used in the culture medium is peptone, soybean peptone, tryptone or beef extract single or compound nitrogen source, and salts are generally added with manganese sulfate, magnesium sulfate and other ions, and the cost of these substances is relatively high , and the composition is complex, sometimes it is not easy to solve the p

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] A kind of butyric acid bacterium fermentation medium, is made up of fish meal, soybean meal, yeast extract, glucose and sodium chloride, by weight 20 parts of fish meal, 10 parts of soybean meal, 0.5 part of yeast extract, 10 parts of glucose, 5 parts of sodium chloride share.

[0051] The preparation method of above-mentioned butyric acid bacteria fermentation medium, concrete steps are as follows:

[0052] (1) The fermentation medium is weighed in g / L as fish meal 20, soybean meal 10, yeast extract 0.5, glucose 10, and sodium chloride 5, wherein the fish meal and soybean meal are crushed to 80 mesh before use;

[0053] (2) Before the medium is prepared, the soybean meal is boiled in water for 30 minutes, filtered through 10 layers of gauze, and the filtrate is added to the medium;

[0054] (3) In the filtrate, weigh and add the remaining medium components in turn, dissolve with deionized water, and adjust the pH to 7.5;

[0055] (4) High-pressure steam sterilization m...

Embodiment 2

[0057] A butyric acid bacterium fermentation medium is composed of fish meal, soybean meal, yeast extract, glucose and sodium chloride, in parts by weight, 15 parts of fish meal, 4 parts of soybean meal, 1 part of yeast extract, 20 parts of glucose, and 4 parts of sodium chloride share.

[0058] The preparation method of above-mentioned butyric acid bacteria fermentation medium, concrete steps are as follows:

[0059] (1) The fermentation medium is weighed in g / L as fish meal 15, soybean meal 4, yeast extract 1, glucose 20, and sodium chloride 4;

[0060] (2) Before the medium is prepared, the soybean meal is boiled in water for 30 minutes, filtered through 10 layers of gauze, and the filtrate is added to the medium;

[0061] (3) In the filtrate, weigh and add the remaining medium components in turn, dissolve with deionized water, and adjust the pH to 7.5;

[0062] (4) High-pressure steam sterilization medium at 121°C for 20min.

Embodiment 3

[0064] A butyric acid bacterium fermentation medium is composed of fish meal, soybean meal, yeast extract, glucose and sodium chloride, in parts by weight, 23 parts of fish meal, 11 parts of soybean meal, 1 part of yeast extract, 15 parts of glucose, and 7 parts of sodium chloride share.

[0065] The preparation method of above-mentioned butyric acid bacteria fermentation medium, concrete steps are as follows:

[0066] (1) The fermentation medium is weighed in g / L as fish meal 23, soybean meal 11, yeast extract 1, glucose 15, and sodium chloride 7, wherein the fish meal and soybean meal are crushed to 80 mesh before use;

[0067] (2) Before the medium is prepared, the soybean meal is boiled in water for 30 minutes, filtered through 10 layers of gauze, and the filtrate is added to the medium;

[0068] (3) In the filtrate, weigh and add the remaining medium components in turn, dissolve with deionized water, and adjust the pH to 7.5;

[0069] (4) High-pressure steam sterilizati...

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PUM

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Abstract

The invention provides a butyric acid bacterium fermentation culture medium and a preparation method thereof and a butyric acid bacterium culture fermentation method. The butyric acid bacterium culture medium includes a nitrogen source, a carbon source and sodium chloride, wherein the nitrogen source is one or any combination of fish meal, bean pulp or yeast extract, and the nitrogen source is glucose and/or corn flour. The number of butyric acid bacteria can stably achieve more than 109CFU/mL after the fermentation culture medium is adopted for fermentation for 14-16h; according to the fermentation culture medium, the nitrogen source and the carbon source which are widely available are used for replacing tryptone, glucose and the like; the fermentation cost is low, the fermentation time is shortened, the fermentation efficiency is improved, and the production cost is greatly lowered.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a butyric acid bacteria fermentation medium, a preparation method thereof and a butyric acid bacteria culture and fermentation method. Background technique [0002] Probiotics have played a positive role in replacing antibiotics, preventing diseases, and improving the environment. However, currently widely used live bacterial preparations generally have shortcomings such as short shelf life and unstable activity. This problem has brought many problems in the practical application of probiotics. difficulty. [0003] As a probiotic, butyric acid bacteria can promote the symbiosis of beneficial bacteria in the intestine and promote the proliferation and development of beneficial bacteria to promote growth. There are protease Ⅰ, protease Ⅱ and lipase in butyric acid bacteria, which can promote the digestion and absorption of fat and protein. Butyric acid bacteria also has th...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/145
Inventor 刘珂飞何玉慧
Owner TIANJIN SHENGJI GRP CO LTD
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