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Staphylococcus aureus chromogenic medium and test piece thereof

A technology of chromogenic medium and staphylococcus, which is applied in the field of microbial inspection and food safety monitoring, can solve the problems of high detection cost, expensive imported chromogenic medium and test strips, inconvenient promotion and use, etc., and achieves high sensitivity and low Detection cost and waste disposal cost, the effect of short detection cycle

Active Publication Date: 2013-09-11
GUANGDONG DAYUAN OASIS FOOD SAFETY TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these imported chromogenic culture media and test strips are expensive, resulting in high detection costs, and it is inconvenient to promote and use them in general grassroots units and enterprises in China.

Method used

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  • Staphylococcus aureus chromogenic medium and test piece thereof
  • Staphylococcus aureus chromogenic medium and test piece thereof
  • Staphylococcus aureus chromogenic medium and test piece thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Each 1000ml medium contains tryptone 5g, yeast extract powder 25g, beef extract powder 2g, glucose 25g, sodium pyruvate 1g, glycine 30g, sodium chloride 1g, potassium dihydrogen phosphate 0.05g, disodium hydrogen phosphate 1g, pH Bacteria mixture 10g, specific enzyme chromogenic substrate 0.05g. Wherein, the antibacterial mixture is composed of sodium azide, aztreonam, deferoxamine and lithium chloride with a mass ratio of 2:1:5:12; the specific enzyme color substrate is 5 with a mass ratio of 3:2. -Bromo-6-chloro-3-indoxyl phosphate and 5-bromo-4-chloro-3-indyloxy-β-D-glucopyranoside.

[0039] Dissolve the above ingredients in 1000mL water, heat to boil, sterilize at 121°C for 15min, and then use it.

Embodiment 2

[0041] Each 1000ml medium contains tryptone 50g, yeast extract powder 1g, beef extract powder 30g, glucose 1g, sodium pyruvate 20g, glycine 2g, sodium chloride 20g, potassium dihydrogen phosphate 4g, disodium hydrogen phosphate 15g, antibacterial Reagent mixture 1g, specific enzyme chromogenic substrate 5g. Among them, the bacteriostatic mixture is composed of sodium azide, aztreonam, deferoxamine and lithium chloride with a mass ratio of 3:1:4:10; the specific enzyme chromogenic substrate is 5 -Bromo-6-chloro-3-indoxyl phosphate and 5-bromo-4-chloro-3-indyloxy-β-D-glucopyranoside.

[0042] Dissolve the above ingredients in 1000mL water, heat to boil, sterilize at 121°C for 15min, and then use it.

Embodiment 3

[0044] Each 1000ml medium contains tryptone 5g, yeast extract powder 12.5g, beef extract powder 2.5g, glucose 9g, sodium pyruvate 1g, glycine 15g, sodium chloride 2.5g, potassium dihydrogen phosphate 3g, disodium hydrogen phosphate 3g , bacteriostat mixture 8g, specific enzyme chromogenic substrate 0.1g. Wherein, the antibacterial mixture is composed of sodium azide, aztreonam, deferoxamine and lithium chloride with a mass ratio of 4:1:8:15; -Bromo-6-chloro-3-indoxyl phosphate and 5-bromo-4-chloro-3-indyloxy-β-D-glucopyranoside.

[0045] Dissolve the above ingredients in 1000mL water, heat to boil, sterilize at 121°C for 15min, and then use it.

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Abstract

The invention discloses staphylococcus aureus chromogenic medium and a test slip thereof. The chromogenic medium contains tryptone, yeast extract powder, beef extract powder, glucose, sodium pyruvate, glycine, sodium chloride, monopotassium phosphate, disodium hydrogen phosphate, a bacteriostatic agent mixture and two specific enzyme chromogenic substrates. The chromogenic medium and the test slip made by using the chromogenic medium can be used for quickly detecting staphylococcus aureus in a large-flux sample, are obvious in positive result color development, accurate in tested result, strong in specificity, high in flexibility, short in test period, simple and convenient to operate, low in test environment requirement and test personnel, and capable of carrying out quantitative analysis while carrying out qualitative test on the staphylococcus aureus, and therefore, the chromogenic media and the test slip are very suitable for being used by grass-roots supervisory authorities and enterprises, and are extensive in application prospect.

Description

technical field [0001] The invention belongs to the field of microbial inspection and food safety monitoring, and in particular relates to a chromogenic medium for rapid detection of Staphylococcus aureus and a test piece made with the chromogenic medium. Background technique [0002] Staphylococcus aureus is widely distributed in nature, and it is one of the important indicators of food hygiene quality. in Salmonella and Vibrio parahaemolyticus. Staphylococcus aureus is also one of the most common pathogens for human infection. It can produce various pathogenic substances such as coagulase, which can cause suppurative inflammation, staphylococcal enteritis and other diseases. Staphylococcus aureus isolated clinically in recent years Almost all are drug-resistant strains that are difficult to suppress, so the bacteria, together with hepatitis and AIDS, are called the world's three major refractory infectious diseases. In order to ensure food safety and protect people's hea...

Claims

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Application Information

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IPC IPC(8): C12Q1/14C12M1/34
Inventor 孙霞卢新
Owner GUANGDONG DAYUAN OASIS FOOD SAFETY TECH CO LTD
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