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Method for preparing bacterial cellulose from corn stalks

A technology of bacterial cellulose and corn stalks, applied in the direction of fermentation, etc., can solve the problems of straw dissolution that have not been reported in detail, and achieve the effect of improving efficiency

Inactive Publication Date: 2011-05-11
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(AMIM)Cl has not been reported in detail for its use in the dissolution of straw

Method used

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  • Method for preparing bacterial cellulose from corn stalks
  • Method for preparing bacterial cellulose from corn stalks
  • Method for preparing bacterial cellulose from corn stalks

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] (1) Pretreatment of corn stalks

[0038] At 100° C. and 500 rpm magnetic stirring speed, 150 mg of 40-60 mesh corn stalks were placed in 1.5 g, 3 g, and 5 g of ionic liquid (AMIM) Cl respectively, and pretreated for 2 hours.

[0039] (2) Regeneration of corn stalks

[0040] Add deionized water 5 times the volume of the ionic liquid to the above-treated cornstalk ionic liquid mixture in the Erlenmeyer flask, stir for 30 minutes, centrifuge the mixture at 4000r for 10 minutes, and wash the precipitate repeatedly until the supernatant is colorless and transparent. Set aside for precipitation.

[0041] (3) Enzymatic hydrolysis of regenerated corn stalks

[0042] Completely transfer 150mg of precipitate to a 50ml Erlenmeyer flask, add 30ml, 50mmol pH 4.8 acetate buffer, then add 90mg of cellulase (11000U / g), put in a shaking water bath, 50°C, 100rpm for enzymolysis reaction After 12 hours, centrifuge at 4,000-10,000 rpm for 5-20 minutes to separate undegraded corn stalks ...

Embodiment 2

[0047] (1) Pretreatment of corn stalks

[0048] 100°C, 500rpm magnetic stirring speed, put 150mg of corn stalks with different meshes (40-60 mesh, 60-80 mesh, 80 mesh or more, mixed mesh) in 50ml of ionic liquid (AMIM)Cl containing 5g In the Erlenmeyer flask, pretreatment 2h.

[0049] (2) Regeneration of corn stalks

[0050] Add deionized water 5 times the volume of the ionic liquid to the above-treated cornstalk ionic liquid mixture for regeneration, stir for 30 minutes, pour the mixture into a Buchner funnel for suction filtration, and rinse the filter residue repeatedly until the filtrate is colorless and transparent, collect Filter residue and filtrate are used for later use.

[0051] (3) Distillation recovery of ionic liquid

[0052] Place the collected filtrate in a distillation flask, and distill under reduced pressure at a temperature of 130°C and a vacuum of -0.09 to -0.1Mpa to remove the water blended with the ionic liquid until no water is evaporated, and the rem...

Embodiment 3

[0059] (1) Pretreatment of corn stalks

[0060] Put 150mg of 40-60mesh corn stalks in 5g of ionic liquid (AMIM)Cl at four temperatures of 90°C, 100°C, 110°C, and 120°C, with magnetic stirring at 500rpm, and treat at 90°C4,6,8 , 10 and 12h; 100°C treatment for 1, 1.5, 2, 2.5 and 3h; 110°C treatment for 20, 40, 60, 80 and 100min; 120°C treatment for 5, 10, 15, 20, 30min.

[0061] (2) Regeneration of corn stalks

[0062] Add 5 times the volume of deionized water to the Erlenmeyer flask, stir for 30 minutes, centrifuge the mixture at 4000 rpm for 10 minutes, wash the precipitate repeatedly until the supernatant is colorless and transparent, collect the precipitate for later use.

[0063] (3) Enzymatic hydrolysis of regenerated corn stalks

[0064] Completely transfer 150mg of precipitate to a 50ml Erlenmeyer flask, add 30ml, 50mmol pH 4.8 acetate buffer, then add 90mg of cellulase (11U / mg), put in a shaking water bath, 50°C, 100rpm for enzymolysis reaction 6h. After the reactio...

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Abstract

The invention relates to a method for preparing bacterial cellulose from corn stalks. The method comprises the following steps: pretreating the corn stalks in an ionic liquid at 90-120 DEG C for 15 minutes-21 hours, then adding deionized water, stirring for regeneration, and adding cellulase to carry out enzymolysis, thereby obtaining an enzymolysis liquid; and detoxifying the enzymolysis liquid to obtain a culture medium for culturing the bacterial cellulose, adding yeast extract and tryptone to obtain a culture medium, adding a seed liquid of bacterial cellulose producing bacteria, and standing to culture at 25-30 DEG C for 8-28 days, thereby obtaining the bacterial cellulose. According to the invention, the farm crop byproduct corn stalks, which are often discarded or incinerated, are utilized as a raw material, thus a cheap carbon source is developed, which reduces the environmental pollution and can be used for producing products with high added value, thereby creating high economic benefit for related industries.

Description

technical field [0001] The invention belongs to the field of bacterial cellulose preparation, in particular to a method for preparing bacterial cellulose with corn stalks. Background technique [0002] Bacterial cellulose (BC) is a kind of cellulose produced by microorganisms. Compared with plant fibers, bacterial cellulose has the characteristics of high crystallinity, high water holding capacity, high Young's modulus, high purity, and excellent biocompatibility. Therefore, bacterial cellulose is used in biomedical materials such as medical dressings, food, and papermaking. , Textile and other fields have a wide range of applications. However, the high production cost of bacterial cellulose limits its industrial scale, so reducing the cost of bacterial cellulose is an urgent problem to be solved in the current industrial production. One of the main factors for the high cost of bacterial cellulose is the high cost of carbon sources in the culture medium. Therefore, this ex...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04
Inventor 洪枫韩士芬曹张军杨雪霞
Owner DONGHUA UNIV
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