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Culture medium used for separating and screening lactic acid bacteria, preparation method and application thereof

A technology for separation, screening and culture medium, which is applied in the field of culture medium for separation and screening of lactic acid bacteria, which can solve the problems of inconspicuous identification of lactic acid bacteria, increased labor load of lactic acid bacteria, and reduced separation and screening efficiency, so as to enhance the inhibition and accuracy of the growth of miscellaneous bacteria High, improve the effect of screening efficiency

Active Publication Date: 2013-09-25
江门市澳保生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the separation and screening medium for lactic acid bacteria is MRS medium, tomato juice agar medium (TJA), etc., but in these mediums, the phenomenon of identification after the growth of lactic acid bacteria is not obvious, the growth of miscellaneous bacteria is too much, and the probability of separation and purification of lactic acid bacteria Low-level problems, which greatly increase the labor load of lactic acid bacteria isolated and purified from nature, reducing the efficiency of isolation and screening

Method used

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  • Culture medium used for separating and screening lactic acid bacteria, preparation method and application thereof
  • Culture medium used for separating and screening lactic acid bacteria, preparation method and application thereof
  • Culture medium used for separating and screening lactic acid bacteria, preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0027] The medium formula used in this embodiment to separate and screen lactic acid bacteria is as follows :Tryptone 10.0g, beef extract 10.0g, yeast extract 5.0g, diammonium citrate 2.0g, glucose 20.0g, Tween-801.0mL, sodium acetate 5.0g, agar 18.0g, calcium carbonate 10.0g, containing formazan Compound acid-base indicator of 0.05g base red and 0.07g bromothymol blue, polymyxin B250000IU, nalidixic acid 0.02g, ascorbic acid 1.0g, L-cysteine-HCl 0.2g, salt solution 100mL , Chenhai 900mL, pH6.8.

[0028] In the medium formula of this embodiment, the formula of the salt solution is: 10.0 g of dipotassium hydrogen phosphate, 10.0 g of potassium dihydrogen phosphate, 2.0 g of magnesium sulfate heptahydrate, 0.5 g of manganese sulfate, 2 g of calcium chloride, and 1000 mL of aged sea water.

[0029] The compound acid-base indicator can be prepared by dissolving methyl red and bromothymol blue in an appropriate amount of ethanol, for example, dissolving 5.0g of methyl red and 7.0g of b...

Embodiment 2

[0041] The medium formula used in this embodiment to separate and screen lactic acid bacteria is as follows :Tryptone 5.0g, beef extract 15.0g, yeast extract 2.0g, diammonium citrate 1.0g, glucose 25.0g, Tween-802.0mL, sodium acetate 5.0g, agar 18.0g, calcium carbonate 10.0g, containing formazan Compound acid-base indicator of base red 0.01g and bromothymol blue 0.01g, polymyxin B500000IU, nalidixic acid 0.02g, ascorbic acid 5.0g, L-cysteine-HCl 0.5g, salt solution 100mL , Chenhai 900mL, pH6.8.

[0042] In the medium formula of this embodiment, the formula of the salt solution is: 10.0 g of dipotassium hydrogen phosphate, 10.0 g of potassium dihydrogen phosphate, 2.0 g of magnesium sulfate heptahydrate, 0.5 g of manganese sulfate, 2 g of calcium chloride, and 1000 mL of aged sea water.

[0043] In the medium formula of this embodiment, the aged seawater is taken from seawater which is far away from the land and is less polluted, and is placed in the dark place for several weeks.

...

Embodiment 3

[0051] The medium formula used in this embodiment to separate and screen lactic acid bacteria is as follows :Tryptone 10.0g, beef extract 10.0g, yeast extract 5.0g, diammonium citrate 2.0g, glucose 20.0g, Tween-801.0mL, sodium acetate 5.0g, agar 18.0g, calcium carbonate 4.0g, containing formazan Compound acid-base indicator of base red 0.5g and bromothymol blue 0.7g, polymyxin B500000IU, nalidixic acid 0.05g, ascorbic acid 1.0g, L-cysteine-HCl 0.1g, salt solution 50mL 950mL of distilled water, pH 6.8.

[0052] The formula of the salt solution in the medium formula of this embodiment is: 10.0 g of dipotassium hydrogen phosphate, 10.0 g of potassium dihydrogen phosphate, 2.0 g of magnesium sulfate heptahydrate, 0.5 g of manganese sulfate, 2 g of calcium chloride, and 1000 mL of distilled water.

[0053] The medium is prepared as follows : It is the same as embodiment 1, and will not be repeated.

[0054] Separation and screening : Collect shells, pufferfish, Macrobrachium rosenber...

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Abstract

The invention provides a culture medium used for separating and screening lactic acid bacteria. The formula of the culture medium comprises the following components: relative to the dosage of 1000ml of a solvent, 5.0-15.0g of tryptone, 5.0-15.0g of beef extract, 2.0-10.0g of yeast extract, 1.0-3.0g of diammonium citrate, 15.0-25.0g of glucose, 0.2-2.0 mL of Tween-80, 2.0-8.0g of sodium acetate, 15.0-20.0g of agar, 2.0-30.0g of calcium carbonate, a compound acid-base indicator containing 0.01-0.5g of methyl red and 0.01-0.7g of bromothymol blue, 0.5-5.0g of ascorbic acid, 0.1-0.5g of L-cysteine HCl, and a selective antibiotic composed of 250000-500000 IU of polymyxin B and 0.01-0.05g of nalidixic acid, wherein the pH value of the culture medium is 6.8; and the solvent is composed of a primary solvent and a secondary solvent, the primary solvent is distilled water or aged seawater, and the secondary solvent is saline solution. The culture medium has the characteristics of being high in accuracy, obvious in authentication phenomenon and the like during separation and screening for lactic acid bacteria.

Description

Technical field [0001] The invention relates to a medium for screening microorganisms, in particular to a medium for separating and screening lactic acid bacteria. Background technique [0002] Lactic acid bacteria refers to a class of non-spore-free, Gram-stain-positive bacteria whose main product of fermented sugar is lactic acid, which grows in anaerobic or facultative anaerobic environment. Lactic acid bacteria is just a customary name, not a taxonomic name for microorganisms. This is a rather complex group of bacteria, classified into hundreds of genera, and it is difficult to use lactic acid production as a classification criterion for bacteria. The morphology of lactic acid bacteria cells is spherical, spheroidal, rod-shaped or short rod-shaped. Current research shows that, except for individual genera, most of lactic acid bacteria are harmless to humans and animals. Gram-positive lactic acid bacteria account for the vast majority, such as Lactobacillus, Pediococcus, and...

Claims

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Application Information

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IPC IPC(8): C12N1/20
Inventor 张宏刚刘陈立张文艳
Owner 江门市澳保生物科技有限公司
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