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Clostridium butyricum as well as colstridium butyricum feed additive and preparation method thereof

A feed additive, Clostridium butyricum technology, applied in biochemical equipment and methods, animal feed, animal feed, etc., can solve the problems of high equipment environment requirements, high production costs, and high product rates, reducing costs and increasing factories. effect of benefit

Inactive Publication Date: 2012-07-11
SHANGHAI CHUANGBO ECOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Clostridium butyricum mainly uses liquid submerged static culture technology. This method has a high yield but high production cost and high requirements for equipment and environment. Its solid-state fermentation culture method is rarely reported.

Method used

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  • Clostridium butyricum as well as colstridium butyricum feed additive and preparation method thereof
  • Clostridium butyricum as well as colstridium butyricum feed additive and preparation method thereof
  • Clostridium butyricum as well as colstridium butyricum feed additive and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Soybean meal was selected as the nitrogen source; a mixture of 90wt% bran and 10wt% molasses was used as the carbon source.

[0021] Step 1, preparation of fermented bacteria seeds and seed activation:

[0022] 1. Prepare a special medium for liquid seeds, including tryptone 2.5g, beef extract 1.5g, yeast extract 0.6g, glucose 0.4g, dipotassium hydrogen phosphate 0.2g, potassium dihydrogen phosphate 0.1g, magnesium sulfate 0.04g, chlorine Calcium 0.02g, ferrous sulfate 0.01g, cysteine ​​hydrochloride 0.05g, distilled water 100ml, adjust the pH to 7.2.

[0023] 2. Inoculate the frozen Clostridium butyricum species into the culture medium, and culture it statically at 37°C for 18 hours.

[0024] Step 2, preparation of solid anaerobic fermentation medium:

[0025] 3. Sterilize the nitrogen source first, and use neutral protease for pretreatment; the weight ratio of nitrogen source to water is 1:2, and neutral protease is added to the mixture of nitrogen source and water ...

Embodiment 2

[0033] A mixture of soybean meal and peanut meal with a weight ratio of 1:1 was selected as the nitrogen source; a mixture of 90wt% bran / rice bran with a weight ratio of 1:1 and 10wt% molasses was used as the carbon source.

[0034] Step 1, preparation of fermented bacteria seeds and seed activation:

[0035] 1. Prepare a special medium for liquid seeds, including tryptone 2.0g, beef extract 1.0g, yeast extract 1.0g, glucose 0.5g, dipotassium hydrogen phosphate 0.2g, potassium dihydrogen phosphate 0.1g, magnesium sulfate 0.04g, chlorine Calcium 0.02g, ferrous sulfate 0.02g, cysteine ​​hydrochloride 0.05g, distilled water 100ml, adjust the pH to 7.4.

[0036] 2. Inoculate the frozen strains into the culture medium, and culture them statically at 38°C for 19 hours in anaerobic culture.

[0037] Step 2, preparation of solid anaerobic fermentation medium:

[0038] 3. Sterilize the nitrogen source first, and use neutral protease for pretreatment; the weight ratio of nitrogen sour...

Embodiment 3

[0046] A total of 60 grown-up binary crossbred boars about 70 days old and weighing about 30kg were selected and randomly divided into two groups for comparative experiments. The boars in the control group were not added this additive, and the additive was added to the ordinary diet at a weight percentage of 5% in the test group. When in use, this additive is fully mixed with ordinary full-price feed, and it can be fed after uniformity. This product needs to be stored in a sealed and dry place, avoiding mixing and stacking with pesticides and strong irritants. The test results are recorded as follows:

[0047] Compare items control group test group Initial weight (kg) 30.57±0.26 30.52±0.23 Final weight (kg) 77.33±8.35 81.10±8.22 Weight gain (kg) 47.07±1.09 51.22±2.01 Average daily feed intake (kg) 2.59 3.18 Feed to meat ratio 2.99 2.51 Feeding cost (yuan / head) 250.20 232．51

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Abstract

The invention discloses a method for preparing a clostridium butyricum feed additive and a clostridium butyricum feed additive prepared by the method. The method for preparing the clostridium butyricum feed additive is characterized by adopting clostridium butyricum (the clostridium butyricum is preserved in the China Center of Industrial Culture Collection and the serial number is CICC 20036). The method comprises the following steps: such materials as tryptone, beef extract, yeast cream, glucose, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, magnesium sulphate, calcium chloride, ferrous sulfate, cysteine hydrochloride and the like are prepared into a dedicated liquid culture medium for seeds; strains are grafted into the culture medium for activating seeds; nitrogen source, carbon source and the like are prepared into a solid anerobic fermentation culture medium; and the seed liquid is grafted into the solid anerobic fermentation culture medium for solid state fermentation and then is dried and sieved. By utilizing the cheap nitrogen and carbon sources to form the medium, the invention provides a novel method for large-scale and industrial production of clostridium butyricum; and the method reduces the cost effectively and increases the benefits of plants.

Description

technical field [0001] The invention relates to a clostridium butyricum and a clostridium butyricum feed additive, belonging to the field of microbial fermentation. Background technique [0002] Clostridium butyricum, also known as Clostridium butyricum, is a Gram-positive anaerobic endophytic bacillus. The buds are huge, and after the formation of the bud robe, one end expands into a drum hammer shape, or the middle part expands into a spindle shape or a shuttle shape. It has no toxic effect on poultry and livestock; through the biological competitive exclusion in the digestive tract of animals, it can inhibit the harmful bacteria in the intestinal tract and promote the growth of beneficial bacteria; it can reduce the ammonia and amine content in the intestines and reduce the burden on the liver; it can produce a variety of vitamins and trace elements; Produce a variety of enzymes, improve feed utilization, and promote animal growth. As a microecological preparation, it h...

Claims

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Application Information

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IPC IPC(8): A23K1/16C12N1/20C12R1/145
Inventor 江瀚马正驰
Owner SHANGHAI CHUANGBO ECOLOGICAL ENG
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