Method of using wheat straws for producing bacterium cellulose
A technology of bacterial cellulose and wheat straw is applied in the field of preparation of bacterial cellulose, which can solve the problems of complex structure of wheat straw, and achieve the effect of low price and wide source.
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Embodiment 1
[0034] Wheat straw is first ground with a plant pulverizer, then soaked overnight (12-24h) with dilute sulfuric acid (3%, w / v) in a reactor at a solid / liquid ratio of 1:6 between the straw and dilute acid solution, Then react at a temperature of 121° C. for 60 minutes, then separate the straw residue and acid solution by suction filtration, collect the hydrolyzate, and refrigerate at 4° C. for later use.
[0035] Add NaOH to adjust the pH value of the hydrolyzed liquid to about 10, filter the precipitate with filter paper to obtain the treated hydrolyzed liquid, and then fine-tune the pH value to 10.0. Then seal it with a film, place it in a 30°C water bath to react overnight, and finally adjust the pH value of the hydrolyzate to 5.0 with dilute acid. Then add 2% (mass percentage) activated carbon, stir (at room temperature for 5-10min), filter out the activated carbon with filter paper to obtain detoxified hydrolyzed clear liquid, and fine-tune the pH value to 5.5 with dilute...
Embodiment 2
[0037] Wheat straw is first ground with a plant grinder, and then soaked overnight (12-24h) in a reactor with dilute hydrochloric acid (1%, w / v) at a solid / liquid ratio of 1:10 straw to dilute acid solution, Then react at a temperature of 121° C. for 75 minutes, then separate the straw residue and acid solution by suction filtration, collect the hydrolyzate, and refrigerate at 4° C. for later use.
[0038] Add Ca(OH) 2 Adjust the pH value of the hydrolyzed liquid to about 10, filter out the precipitate with filter paper to obtain the treated hydrolyzed liquid, and then fine-tune the pH value to 10.0. Then seal it with a film, place it in a 40°C water bath to react overnight, and finally adjust the pH value of the hydrolyzate to 5.0 with dilute acid. Then add 2% (mass percentage) activated carbon, stir (at room temperature for 5-10min), filter out the activated carbon with filter paper to obtain detoxified hydrolyzed clear liquid, and fine-tune the pH value to 5.5 with dilute ...
Embodiment 3
[0040] Wheat straw is first ground with a plant pulverizer, and then soaked overnight (12-24h) with dilute sulfuric acid (2%, w / v) in a reactor with a solid / liquid ratio of 1:15 straw to dilute acid solution, Then react at a temperature of 100° C. for 80 minutes, then separate the straw residue and acid solution by suction filtration, collect the hydrolyzate, and refrigerate at 4° C. for later use.
[0041] Add 25% ammonia water to adjust the pH value of the hydrolyzed liquid to 10, filter out the precipitate with filter paper to obtain the treated hydrolyzed liquid, and fine-tune the pH value to 10. Then seal it with a film, place it in a 25°C water bath to react overnight, and finally adjust the pH value of the hydrolyzate to 5.0 with dilute acid. Then add 2% (mass percentage) activated carbon, stir (at room temperature for 5-10min), filter out the activated carbon with filter paper to obtain detoxified hydrolyzed clear liquid, and fine-tune the pH value to 5.5 with dilute s...
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