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Selective culture medium used for quantitative detection of bacillus

A technology for quantitative detection of bacillus, applied in the direction of microorganism-based methods, measurement/inspection of microorganisms, bacteria, etc., can solve the problems of high cost, inability to detect the total number of bacillus, low accuracy, etc., and achieve the elimination of microorganisms or impurities, Rapid and accurate quantitative detection of Bacillus and the effect of eliminating influence

Active Publication Date: 2011-07-20
清远海贝生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a low-cost, high-accuracy method according to the problems of low accuracy, inability to detect the total number of bacillus and high cost when using fluorescent quantitative PCR in the existing quantitative detection of bacillus. Selective medium for quantitative detection of Bacillus

Method used

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  • Selective culture medium used for quantitative detection of bacillus
  • Selective culture medium used for quantitative detection of bacillus
  • Selective culture medium used for quantitative detection of bacillus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Detecting the number of bacillus bacteria in the brackish water shrimp pond

[0017] The bacillus content was 20 x 10 8 The CFU / g product was used in the Penaeus vannamei pond at a dose of 1ppm, and samples were taken to detect the number of bacillus in the pond water after 1 day. The specific detection method is as follows:

[0018] (1) Sample pretreatment:

[0019] Water samples were taken for gradient dilution, and three dilutions of 1×, 10×, and 100× were taken for the variable temperature culture group for use; three dilutions of 100×, 1000×, and 10000× for the constant temperature culture group were used for use.

[0020] (2) Preparation of selective medium plate

[0021] Prepare solid culture according to the following formula: glucose 10g / L, yeast extract 5g / L, tryptone 10g / L, calcium chloride 1 g / L, magnesium sulfate 0.5 g / L, polymyxin sulfate 200IU / L and agar 15 g / L, adjust the pH to 7.2, and sterilize at 121°C for 20 minutes. After the medium ...

Embodiment 2

[0030] Example 2 Detection of Bacillus counts in shrimp ponds in elevated ponds

[0031] The bacillus content was 20 x 10 8 The CFU / g product was used in the elevated pond of Penaeus vannamei at a dose of 1ppm, and the number of bacillus in the pond water was sampled and tested 1 day later. The specific detection method is as follows:

[0032] (1) Sample pretreatment:

[0033] Water samples were taken for gradient dilution, and three dilutions of 1×, 10×, and 100× were taken for the variable temperature culture group for use; three dilutions of 100×, 1000×, and 10000× for the constant temperature culture group were used for use.

[0034] (2) Preparation of selective medium plate

[0035] Prepare solid culture according to the following formula: glucose 5g / L, yeast extract 3g / L, tryptone 5g / L, calcium chloride 1 g / L, magnesium sulfate 0.5 g / L, polymyxin sulfate 200IU / L and agar 15 g / L, adjust the pH to 7.2, and sterilize at 121°C for 20 minutes. After the medium is coo...

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Abstract

The invention discloses a selective culture medium used for the quantitative detection of bacillus. Each litre of the selective culture medium comprises the following components: 1-10g of glucose, 1-5g of yeast extract, 5-10g of tryptone, 0.2-2g of calcium chloride, 0.1-1g of magnesium sulfate, 15-20g of agar and 100-500IU of bacteriostatic agent. Through variable temperature cultivation, the selective culture medium adopts can be used to increase the selectivity of the conventional plate counting method to bacillus, eliminate the influences of other microorganisms or impurities in s sample to be detected and achieve the aim of detecting bacillus fast and quantitatively.

Description

technical field [0001] The invention relates to the field of bacillus detection, in particular to a selective medium for quantitative detection of bacillus. Background technique [0002] With the deterioration of the breeding environment, water quality problems in the breeding process have become increasingly prominent. At present, Bacillus preparations are widely used in the aquaculture industry as the main water quality improvement products. The growth and reproduction of bacillus in the culture pond is the prerequisite for the function of bacillus. At the same time, the change of the number of bacillus in the pond is the basis for determining the frequency of product use. Therefore, it is very important to track and detect changes in the number of Bacillus in the pond. But because there are many kinds of bacteria in the pond, and the bacillus often does not belong to the dominant population in the water body, the number of the bacillus is often all in 10 4 Below CFU / m...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12Q1/06C12R1/07
Inventor 张家学
Owner 清远海贝生物技术有限公司
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