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Manufacturing method of purple puffball liquid strain

A technology of purple puffballs and production methods, applied in botany equipment and methods, horticulture, applications, etc., can solve the problems of high pollution rate of purple puffballs, long growth cycle, complicated process, etc., and achieve production cost reduction, The effects of reduced bacterial contamination and simple production methods

Inactive Publication Date: 2014-05-28
GUIZHOU INST OF BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the problems of high pollution rate, long growth cycle and complicated process of existing purple puffball strains, and to provide a new method for making purple puffball liquid strains, so that the purple puffball produced can be Strain process is simple, pure strain, less pollution, simple operation, short cycle (6 ~ 8d)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1, a kind of preparation method of purple puffball liquid strain, is characterized in that: comprise the following steps

[0016] (1) Culture medium production: Calculated by weight, cut 150 parts of mature purple puffball fruiting bodies into blocks, 150 parts of peeled potatoes and cut into blocks, add blocky purple puffball fruiting bodies and blocky potatoes Boil 1000 parts of water for 30 minutes, filter the purple puffball fruiting bodies and potato residues with gauze to obtain the filtrate, add 5 parts of tryptone, 0.45 parts of carboxamide, 8 parts of glucose, 0.55 parts of beef extract, and corn kernels into the filtrate 8 servings, vitamin B 1 0.000031 parts, 0.50 parts of dipotassium hydrogen phosphate, 0.50 parts of magnesium sulfate, 0.60 parts of calcium chloride, add water to 1000 parts, and control the pH value to 5.5-6.5;

[0017] (2) Sterilization of medium: divide the prepared medium into Erlenmeyer flasks, 150ml per bottle, cover with cotton plugs,...

Embodiment 2

[0021] 1, a kind of preparation method of purple puffball liquid strain, is characterized in that: comprise the following steps

[0022] (1) Culture medium production: calculated by weight, cut 200 parts of mature purple puffball fruiting bodies into blocks, 200 parts of peeled potatoes and cut into blocks, add blocky purple puffball fruiting bodies and blocky potatoes Boil 1000 parts of water for 40 minutes, filter the purple puffball fruiting bodies and potato residues with gauze to obtain the filtrate, add 8 parts of tryptone, 0.50 parts of carboxamide, 10 parts of glucose, 0.65 parts of beef extract, and corn kernels into the filtrate. 10 servings, vitamin B 1 0.000033 parts, 0.70 parts of dipotassium hydrogen phosphate, 0.60 parts of magnesium sulfate, 0.70 parts of calcium chloride, add water to 1000 parts, and control the pH value to 5.5-6.5;

[0023] (2) Sterilization of medium: divide the prepared medium into Erlenmeyer flasks, 200ml each, cover with cotton plugs, st...

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Abstract

The invention discloses a manufacturing method of a purple puffball liquid strain, which is formed in a mode that purple puffball sporocarps, potato, trypsin peptone, carbamide, glucose, beef extract, corn kernels, vitamin B1, dipotassium phosphate, magnesium sulfate, calcium chloride and the like are used for manufacturing a purple puffball liquid medium which is subjected to medium degerming, inoculating and strain culturing. The manufacturing method has prominent characteristics compared with the prior art. The manufacturing method of the purple puffball liquid strain is simple, the production cost is reduced by more than 20%, and the strain pollution is reduced by more than 30%; the myceilum biomass (125.55mk / kg) is higher than that of the traditional method by 20mg / kg, and the infection intensity (62%) of the strain to trees is higher than that of the traditional method by more than 15%. The method can realize the scale and industrialization production of the purple puffball liquid strain and develops a new wealth acquiring route for mountain area peasants in Guizhou Province.

Description

technical field [0001] The invention relates to the technical field of producing edible and medicinal fungus strains, in particular to a preparation method of purple puffball liquid strains. Background technique [0002] Purple Puffball ( Calvatia lilacina (Mont. et Berk.) Lloyd.) The fruiting body is top-shaped, small, usually 5-12 cm in diameter, the upper part is flat and round to produce spores, the lower part is sterile, forming a long cylindrical stalk, also known as the stalked puffball; After the spores are mature, the upper part is completely broken, leaving only the cup-shaped sterile base. Spores are spherical, 4-5.5 microns in diameter, with small spines; sporocysts are very long, branched, intertwined, pale in color, 2-5 microns thick. Symbiosis with trees can improve the resistance of trees and promote the growth and development of trees. [0003] Purple puffball contains sodium phosphate, puffball element (gemmatein, C 17 h 12 o 7 ), ergosterol, leucine ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 贺红早张珍明刘盈盈王莹任春光孙超张玉武
Owner GUIZHOU INST OF BIOLOGY
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