Low serum efficiency mycoplasma gallisepticam attenuated strain culture medium and preparation method thereof
A technology of Mycoplasma gallisepticum and culture medium, applied in the field of veterinary biology, can solve the problems of affecting the immune efficacy of vaccines, increasing allergic stress reactions, etc., and achieve the effect of reducing allergic stress reactions and increasing the titer of live bacteria
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Embodiment 1
[0024] The preparation of culture medium of the present invention
[0025] Basal medium:
[0026]
[0027] Sterilize at 116°C for 30 minutes. After cooling, add the following auxiliary medium components under aseptic conditions to make a culture medium for Mycoplasma gallisepticum.
[0028] Auxiliary medium:
[0029]
[0030]
[0031] Dissolve the components of the basal medium (1)-(8) one by one into 500ml distilled water for high-pressure sterilization, add auxiliary medium after cooling, and adjust the pH to 7.7 with 1mol / L sodium hydroxide solution to obtain low-serum and high-efficiency Mycoplasma gallisepticum culture base.
Embodiment 2
[0033] The preparation of culture medium of the present invention
[0034] Basal medium:
[0035]
[0036] Sterilize at 116°C for 30 minutes. After cooling, add the following auxiliary medium components under aseptic conditions to make a medium for cultivating attenuated strains of Mycoplasma gallisepticum.
[0037] Auxiliary medium:
[0038]
[0039] Dissolve the components of the basic medium (1)-(8) one by one in 800ml distilled water and sterilize under high pressure, add auxiliary medium after cooling, and adjust the pH to 7.7 with 1mol / L sodium hydroxide solution to obtain low-serum and high-efficiency Mycoplasma gallisepticum culture base.
Embodiment 3
[0041] Use Mycoplasma gallisepticum (F36) to carry out comparison culture test to Mycoplasma gallisepticum (F36) by Mycoplasma medium of the present invention, improved Frey's medium, Mycoplasma medium respectively:
[0042] 1 Preparation of culture medium of the present invention
[0043] Basal medium:
[0044]
[0045]
[0046] Sterilize at 116°C for 30 minutes. After cooling, add the following auxiliary medium components under aseptic conditions to make a culture medium for Mycoplasma gallisepticum.
[0047] Auxiliary medium:
[0048]
[0049] Dissolve the components of the basic medium (1)-(8) one by one in 1000ml distilled water and sterilize under high pressure, add auxiliary medium after cooling, and adjust the pH to 7.7 with 1mol / L sodium hydroxide solution to obtain low-serum and high-efficiency Mycoplasma gallisepticum culture base.
[0050] 2 Preparation of Modified Frey's Medium
[0051] base fluid
[0052]
[0053] Dissolve in 1000ml deionized wat...
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