Mycoplasma gallisepticum culture medium and preparation method thereof

Abstract

The invention relates to a mycoplasma gallisepticum culture medium and a preparation method thereof. The mycoplasma gallisepticum culture medium comprises a basic culture medium and auxiliary components, wherein the basic culture medium is prepared from a phosphate buffer solution, PPLO broth, glucose, yeast extract powder, phenol red and trehalose; and the auxiliary components comprise pig serumand penicillin of 800,000 units / ml. According to the culture medium, the antigen content of the mycoplasma avium bacterial liquid is remarkably improved, and the titer of the bacterial liquid is 10<14-15>CCU / ml and is higher than the quality standard of existing vaccine semi-finished bacterial liquids. The preparation method can increase the antigen content of mycoplasma gallisepticum, simplify the production process and reduce the production cost.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a culture medium for Mycoplasma gallisepticum and a preparation method thereof. Background technique [0002] Mycoplasma gallisepticum (Mycoplasma galliseptium, MG) infection, also known as chicken chronic respiratory disease (chronic respiratory disease, CRD), is widely prevalent in all poultry-raising countries in the world. After the chicken farm is infected with MG, the weak chick rate of the chicks increases, the egg production rate of the laying hens, the body weight of the broiler chickens and the feed conversion rate all decrease, and it is prone to secondary bacterial or viral infections, resulting in an increase in the mortality rate of the chickens. The development of the industry has caused serious harm. Vaccination is the most important measure to prevent and control the disease. Although purification is difficult, it is the only measure to eradicate the disease. [000...

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Problems solved by technology

[0004] However, the existing traditional culture medium MG has a low gradient of live bacteria (at 1.0×10 8-10 CCU / ml), the culture time is long for 24-48h, the antigenic protein concentration is low (30-50ug / ml), the bacterial solution (semi-finished product) used for seedling production cannot be used directly when producing inactivated vaccines, and the concentration operation of the semi-finished product is heavy and complicated. The loss in the concentration process is large, which ultimately affects the protective efficacy of the finished vaccine, and the production cost of the laboratory vaccine is increased. The serum added to the existing traditional medium for vaccine production is generally between 10-30%. Chickens increase the allergic stress response of heterologous bodies to chickens, which ultimately affects the immune efficacy of the vaccine and increases production costs: when the existing traditional medium is used for MG isolation, the pathogen detection rate is low and the growth is slow (needed for the initial isolation) The average time is 7-12d), which is not conducive to diagnosis and clinical drug screening

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