33 results about "Commensalism" patented technology

The invention discloses a matched technology for breeding ducks in a rice field. The matched technology comprises a duck group management technology, a rice culture technology and a plant protection technology. The specific technical method comprises the following steps that: the duck group management technology comprises the technologies of building a fence net and stocking a duck group; the rice culture technology comprises a rice field selecting technology, a rice variety selecting technology, a rice transplanting technology and a field management technology; and the plant protection technology comprises an administration technology during seedling transplanting and a field management technology after the duck group leaves the field. A duck shed and a rest platform are arranged at the two ends of the field respectively and different living regions are arranged interlacedly, so that the times of the ducks going back and forth in the field are increased, chances of finding food are increased and the aim of preventing and controlling rice diseases and insect damage. In addition, trap lamps are arranged in the field, so that moths and other insects can be effectively captured and killed and occurrence probability of diseases and insect damage is further reduced. Rice and duck commensalism technologies are integrated. The technologies are normative, matched and associated with one another and high in operability, and contribute to standardized production of rice and duct products.

A composite inoculant for degrading petroleum is characterized by comprising acinetobacter venetianus LCL-1 and dietzia sp. CN-3, wherein the ratio of live acinetobacter venetianus LCL-1 to live dietzia sp. CN-3 is 1-50:10-200. The strain of acinetobacter venetianus LCL-1 is preserved in China Center for Type Culture Collection (CCTCC), the preservation number is M 2015538; and the strain of dietzia sp. CN-3 is preserved in China Center for Type Culture Collection (CCTCC), and the preservation number is M 2015537. The acinetobacter and dietzia are separated from soil polluted by petroleum, both have an efficient petroleum degrading performance, and can well cooperate with each other. Through the relationships such as commensalism, mutualism, and the like, the shortage of low degradation rate of single bacteria is overcome, and the petroleum degrading performance is largely improved. Moreover, the invention also provides a preparation method of the composite inoculant and an application of the composite inoculant in petroleum degradation.

The invention discloses an ecological planting method of oryza sativa. The method comprises the steps of paddy field selection, field preparation, seed treatment, sowing and seedling raising, seedlingtransplanting, insect attack treatment, fertilizer management, irrigation management and harvesting. According to the ecological planting method of the oryza sativa, ducks, carps and frogs are stocked in the field, not only is deinsectization carried out, but also the point is that a biologic chain with characteristics of species commensalism and cyclic utilization is formed in the field, duck manure fertilizes the field, thereby not only multiplying fodder biology to be taken by the ducks, but also promoting the growth of the oryza sativa; the rice has good taste, and high content of nutrient substances.

The invention discloses a porosity measuring method for resource storage commensalism type stratums. The porosity measuring method mainly includes the steps of accurate homing of a core depth based on physical properties of rock and nuclear magnetic resonance logging, a multi-disciplinary and multi-scale data combination organic carbon content (TOC) modeling method, a porosity sensitive parameter optimization scheme based on source rock control, and a porosity fine modeling method based on staging of the organic carbon content (TOC). According to the porosity measuring method, influences of source rock to logging responses are considered, the organic carbon content is calculated firstly, then the resource storage commensalism type stratums are staged according to the organic carbon content, and different computational formulas are adopted for calculating the porosity according to the divided stages; the porosity obtained with the method is quite accurate, and the mean absolute error, the average relative error, the correlation coefficient and the like reach the standard and the rule of tight oil logging evaluation.

The invention discloses a leech fry, snail and aquatic plant commensalism intermediate breeding method and belongs to the technical field of aquaculture. According to the method, an ecotype leech fry commensalism breeding system is built, small snails and / or zooplanktons and / or nutrients are adopted as fodder of young leeches, the breeding system is stabilized through the purification capacity of aquatic plants, deterioration of water quality is avoided, the young leeches obtain rich nutrition through composite collocation and application of small snails, zooplanktons and nutrients, leech fries are good in activity, rapid in growth and low in casualty rate, the situation that as young leeches live in groups and climb to be groups seriously, serious death is caused when the young leeches can not obtain fodder is avoided, the rate of survival is improved in the leech fry breeding stage, the method is simple in process, technical feasibility is achieved, breeding enterprises and farmers can use the method conveniently, the technical advantages of maintaining good and stable breeding environment, reducing the risks of leech fry death and the like are achieved, and the method has wide application prospects in the field of national leech breeding.

The invention provides a method for producing tetrodotoxin from microorganisms by commensalism culture fermentation. The method is characterized by comprising the following steps: preparing microorganism strains which are separated from veneniferous tissues of a wild puffer into bacterial suspensions by using sterile distilled water, and mixing the bacterial suspensions and inoculating into a same liquid culture medium to carry out seed liquid culture for 1-3 days; and inoculating 5-10% inoculation amount of seed liquid into a fermentation culture medium to carry out fermentation culture for 2-7 days, wherein the liquid culture medium comprises the following components by weight percent: 0.1-0.5% of yeast extract, 0.1-0.5% of beef extract, 0.1-0.5% of peptone, 0.5-2% of glucose, 0-1% of sugar and the balance of seawater, and the pH value is 6.5-7.2; and the seed liquid is subjected to fermentation culture under the conditions that the temperature is 24-30 DEG C and the rotation speed is 150-200 rpm (revolutions per minutes). According to the method provided by the invention, a micro-ecological environment in which the microorganisms producing toxicity in the puffer is located can be well simulated, thereby being beneficial to the adaptability of the microorganisms producing toxicity to culture in vitro, finally being beneficial to growth and toxicity production of the microorganisms producing toxicity and solving the bottleneck that at present, the yield of the tetrodotoxin produced by microbial fermentation is low in general.

The invention relates to the technical field of planting, in particular to a method for interplanting cassia alata Linn and stylosanthes guianensis. According to the method, cassia alata Linn and stylosanthes guianensis are interplanted, and the super-strong cadmium-rich capability of the stylosanthes guianensis is utilized to achieve the purpose of improving the cadmium content in soil. In addition, the super-strong nitrogen fixing capacity of the cassia alata Linn is also utilized to provide enough nitrogen element for the soil, and growth and commensalism of stylosanthes guianensis and cassia alata Linn is ensured. Cassia alata Linn branches and stylosanthes guianensis seeds are preprocessed, the germination rate of the cassia alata Linn and the rooting rate of the stylosanthes guianensis can be greatly improved, growth of the cassia alata Linn and stylosanthes guianensis is effectively promoted, commensalism of stylosanthes guianensis and cassia alata Linn is achieved, accordinglysoil fertility supplementation is accelerated, and thus the purpose of improving the cadmium content in soil is achieved.

The invention provides a method for fast forming a microalgae-aerobic sludge particle commensalism system at a high speed in natural light. The method is characterized by comprising the following steps of inoculating a reactor with mature aerobic particle sludge; putting the reactor in a position facing the sun; receiving 10 hours or more of natural light illumination every day; using an anaerobic / aerobic / anoxic mode for operation, wherein according to the anaerobic / aerobic / anoxic mode, the water inlet lasts 2 to 10min, the anaerobic treatment time is 100 to 150min, the aerobic treatment timeis 90 to 120min, the anoxic treatment time is 60 to 164min, the sedimentation time is 2 to 10min, the water discharge time is 2 to 10min, the aeration rate of an aerobic section is 270 to 330 mL / min,and the stirring speed is 200 to 300 r / min; thus obtaining the mature microalgae-aerobic sludge particle commensalism system in the 7th day. The method has the advantages that the culture time of themicroalgae-aerobic sludge particles in the prior art is greatly shortened; the sewage purification effect and the anti-impact capability are promoted; the self oxygen generation operation of the commensalism system is realized.

The invention discloses a method for raising the cotton yield and plantation benefits of high saline and alkaline land. The method comprises the following steps of flattening and desalinating soil in winner; covering the soil with a mulch film in early spring; applying a fertilizer in the last ten-day of April and early May, wherein the fertilizer applied to each hectare of land ranges from 90 kg to 100 kg when being converted into the pure N amount, the P<2>O<5> amount ranges from 42 kg to 45 kg, the K<2>O amount ranges from 30 kg to 35 kg, and a loss-controlled fertilizer mixed with attapulgite is selected as the fertilizer variety; conducting cotton sowing after fertilizer application is ended; after cotton seedlings emerge, conducting seedling final signaling and filling the gaps with seedlings in time, wherein the number of plants per mu is 42-45 thousand plants / hm<2>; during the period when three leaves emerge out of each cotton seedling in early June, intercropping soybeans among cotton lines; harvesting the soybeans in the last ten-day of September, and picking the first batch of ripe unginned cotton. According to the method, by improving the fertilizer application technology and adopting cotton and soybean intercropping, commensalism growth of cotton crops and soybean crops is synergized, fertilizer losses are reduced, and the saline and alkaline land soil is restored. Contrast tests show that compared with a traditional method, by means of the method, the cotton yield and the soybean yield can be obviously raised.

Provided is a rice, soft-shelled turtle and fish commensalism system and breeding method, and relates to the technical field of rice cultivation and aquaculture. According to the rice, soft-shelled turtle and fish commensalism system, young soft-shelled turtles and fries are put in a paddy planting field to form the rice, soft-shelled turtle and fish commensalism system. The system is different from a previous circular furrow type paddy filed adopted to breed the soft-shelled turtle, and by using the previous method, mechanical harvest is carried out on the paddy field by means of ditching work from one side. The system has the advantages that the operation is convenient and simple, the investment cost is reduced, and it is convenient for staffs to feed and manage the soft-shelled turtles,and inspecting conditions of eating and activity and the like are convenient, during feeding the soft-shelled turtles. The rice, soft-shelled turtle and fish commensalism system improves economic benefits per unit area, the comprehensive economic benefits reach thousand catties of grains and ten thousand yuan per mu, crucian is introduced in the rice and soft-shelled turtle system, and the economic benefits per unit area are significantly improved. By reasonably balancing nutrient substance, antibiotic is not involved in prepared manual preparing feed, the nutrient components are comprehensive and balanced, the feed with delicate taste can significantly increase appetite, promote production and weight, and also significantly improve the immunity, the growth rate, and the survival rate.

The invention discloses a method for searching a mixed biological symbiosis for single-objective optimization, which comprises the following steps: initializing the number of individuals N, the dimension D of the population, the parameters related to the maximum number of function evaluation times, and randomly generating the initial population; For fitness evaluation, select the best individual among them; update the population by adopting the mutual-benefit symbiosis strategy; update the population by adopting the partial-benefit symbiosis strategy; update the population by adopting the parasitic-symbiosis strategy; judge whether the termination condition is met, and then output the optimal individual and fitness value , if not, continue to repeat the three symbiotic strategies to update the population. The method of the present invention can obtain the solution required by the problem in a small number of iterations in the actual problem solving, which reduces the time required for solving the problem to a certain extent.

The invention discloses an immobilized microalgae reactor taking mycelium pellets as the carriers and a wastewater processing method. The reactor comprises a water inlet system, a reactor body, and awater outlet system. The water inlet system comprises a water inlet box. The water inlet box is sequentially connected to a water pump and a water inlet pipe. The reactor body comprises an uprising pipe and a descending pipe. The other end of the water inlet pipe is communicated with the top end of the descending pipe. The uprising pipe is arranged in the cavity of the descending pipe. The bottomof the descending pipe is provided with an aeration head. The aeration head is communicated with an air compressor through an air inlet pipe. The water outlet system comprises a water outlet box and awater outlet pipe. The water outlet pipe is installed on the lateral wall of the descending pipe. The water outlet pipe is communicated with the descending pipe. Water from the water outlet pipe is introduced into the water outlet box. The wastewater processing method comprises following steps: manufacturing a reactor, preparing a condensed alga solution, mycelium pellet suspension, flocculant fermentation broth, and photosynthetic bacteria concentrate, and rapidly starting and running a bacterium-alga commensalism system. The problems that the time for generating a mycelium pellet-microalgaeparticle system is long and the stability of the system is bad are solved.

The invention relates to a novel biological floc. The biological floc includes microalgae aggregates and commensalism heterotrophic bacteria, and the commensalism heterotrophic bacteria are Pesudomonas sp. and Bacillus sp. The biological floc significantly improves the water quality of the nursing pond of the litopenaeus vannamei, improves the survival rate, growth index and immunity of the litopenaeus vannamei, reduces the application amount of compound feed and external carbon sources, and reduces the aeration intensity and quantity of exchanged water.

The invention relates to the technical field of soilless culture, in particular to a fish vegetable commensalism water fertilizer processing system and method. The fish vegetable commensalism water fertilizer processing system includes a primary solution storage device, a pressure mixing device, a slow releasing device, a spraying device, and a control device; the primary solution storage device is connected to a material inlet of the pressure mixing device through a first pipe, solenoid valves are arranged on the first pipe; the slow releasing device and the spraying device are connected to two material outlets of the pressure mixing device through pipes respectively; first proportional valves are arranged on the pipes; the solenoid valves and the first proportional valves are connected to the control device. The slow releasing device is started to apply a fertilizer in an early stage and a ripe stage of a crop; the slow releasing device and the spraying device are started in a vigorous stage of the crop, the slow releasing device is started to supply a macroelement nutriment solution, the spraying device is started to supply a microelement nutriment solution, accurate high efficiency fertilization and fertilizer supplement can be achieved, the fertilizer can be efficiently utilized, the yield and the quality of vegetables can be improved, the growth time of the vegetables is shortened, the working load is reduced, and intelligent accurate water fertilizer management can be achieved.

The invention discloses feed composite multi-microfunctional microbial inoculums. The feed composite multi-microfunctional microbial inoculums are obtained by mixing lactic acid bacterium mixed bacterial liquid, saccharomycetes mixed bacterial liquid, bacillus mixed bacterial liquid and a thallus protective agent, wherein the mass ratio of the components is 5:2:1:2. Application of the feed composite multi-microfunctional microbial inoculums in the field of feed is that the feed composite multi-microfunctional microbial inoculums are applied to the conventional feed according to 10 kg per ton of feed. The used bacterial strains are probiotics which are allowed to use through audit of the Department of Agriculture, and can exert special effect in the intestinal track of a pig through long-time production practice; and the probiotics can completely exert the effect through commensalism, breeding, competition or phagocytosis, have high propagation speed of the thallus, have strong competitive advantage, can perform industrialized large-scale production, have high survival rate and high stability after use, and can promote the growth and development of the pigs, prevent diseases, inhibit release of harmful gas and reduce mosquitoes and flies in a colony house.

The invention discloses a system for preparing a biomass monomer through microbial coculture. With biomass as a substrate, through process regulation and control over special equipment, coculture of biomass degrading microbes and target monomer generating microbes is achieved to prepare a target monomer. The system is characterized in that by coculturing rumen microbes and target product microbes and by regulating and controlling the equipment capable of separating a product in situ, commensalism of the rumen microbes and the target product microbes and high-efficiency preparation of a target product are achieved.

The invention relates to a complex microbial inoculant used for degrading waste water rich in surfactant. Four strains, including bacillus pumilus, bacillus subtilis, bacillus cereus and bacillus megatherium, are used and are subjected to slant culture, shake-flask culture, seed tank culture, fermentation tank culture, centrifugation and spray drying processing to obtain finished product bacterial powder of each strain. When the complex microbial inoculant is used, four kinds of bacterial powder are regulated and formed according to a ratio between nonionic surfactant and ionic surfactant in the waste water, then, the bacterial powder is put into a facultative tank and an aeration tank of a waste water biodegradation system; each bacterial powder contains a great quantity of active spores, and the active spores can quickly form a commensalism cooperative system; within 30h, the degradation of non-ions can be 90% or above, the degradation of anionic surfactant can be 99% or above, the integral degradation of COD (Chemical Oxygen Demand) in the waste water can be 95% or above, and discharged water can achieve a secondary discharged standard. The complex microbial inoculant is an efficient sewage processing biological enhancement technology without secondary pollution.

The invention discloses a breeding method for tea garden free-ranging black native chicken. The breeding method includes the following steps of site selecting, wherein a tea garden which free water flows through and has a 30-degree slope is selected; barton constructing and equipment installing, wherein it is required that a barton is good in lighting effect, ventilating effect, and flat in ground, coops, water dispensers, feeding barrels, psychrometers and warm keeping stoves are arranged; variety selecting, wherein one-month-old black native chicken variety which is good, resistant diseasesand healthy is selected for breeding; feeding and managing, wherein black native chicken is put into the tea garden in the daytime and driven into the barton at night every day; drinking managing, wherein black native chicken is made to drink water before being put into the tea garden on the morning every day; disease preventing, wherein the barton and activity places around the barton are disinfected every week. The black native chicken is bred in the tea garden in a free-ranging mode, the black native chicken can catch and feed on insects on tea trees, the effect of removing tea garden disease and insect damage is achieved, the ecological effect of tea-chicken commensalism is achieved, and a good growth environment is created for black native chicken breeding.

The invention relates to a method for producing distiller's koji for refreshing Shanlan rice wine. The invention relates to a strain of Rhizopus oryzae strain GIM3208, a strain of Saccharomyces cerevisiae GIM245 and distiller's yeast containing the strains. A method for producing refreshing Shanlan yellow rice wine koji, comprising: inoculating the rhizopus strain on a culture medium with bran or rice bran as the main raw material, and carrying out solid culture to obtain pure rhizopus koji; inoculating the rhizopus strain Inoculate with yeast strains in a culture medium with bran or rice bran as the main raw material, and carry out mixed solid culture to obtain a yeast-based solid mixed koji. The pure rhizopus koji and the mixed koji are mixed in a certain ratio to obtain the refreshing koji of Shanlan rice wine. The rhizopus strain and the yeast strain described in the present invention have a favorable symbiotic relationship during mixed culture. In the preparation process of pure rhizopus koji and mixed koji, a certain amount of betel leaves, mulberry leaves and mugwort leaves are added to loosen and ventilate the koji making process, which is beneficial to the growth of rhizopus and yeast, and can also play a role Inhibit the growth of bacteria. At the same time, the unique aroma of distiller's yeast is increased.

The invention relates to a mushroom spawn stick which comprises raw materials including, in weight percent, 0-20% of cottonseed hulls, 0-35% of wheat bran, 1-2% of gypsum and saw dust added to 100%. A mushroom is excellent in quality, large in mushroom body, high in yield, low in cost and large in cultivation space, water, land and fertilizers are saved, soil nutrients can be increased, and forest and grass spawn commensalism is promoted. In addition, the mushroom is less in raw material composition, simple and easy to manufacture and low in cost, large-scale cultivation, high yield and excellent quality of the mushroom can be realized, and the economic efficiency of mushroom cultivation is greatly improved.

The invention relates to a pleurotus nebrodensis cultivating bar and a preparation method thereof. The pleurotus nebrodensis cultivating bar comprises the following raw materials in percentage by weight: 40%-60% of corncobs, 15%-25% of cottonseed hulls, 5%-12% of wheat bran, 10%-20% of plant ash, 2%-5% of Shixun and 2%-4% of brown sugar. By virtue of scientific compatibility of the cultivating bar, cultured pleurotus nebrodensis has the advantages that the quality is excellent, mushroom bodies are big, the yield is high, the cost is low, the cultivation space is large, and the water, land and fertilizer are saved; by adding the plant ash, soil nutrients are increased, and the commensalism of the forest and the grass fungus is promoted.

The invention relates to a method for preparing a cordyceps sinensis strain material with high infection activity by utilizing a cultivation chamber. According to the method, a psychrophyte tissue culture seedling cultivation chamber of which the climatic conditions can be controlled automatically or manually is established; the climatic factors such as the temperature, the humidity, the illumination and the like of the Qinghai-Tibet Plateau are simulated; psychrophyte tissue culture seedlings are obtained by using a tissue culture rapid propagation and cultivation technology; the prepared cordyceps sinensis resource is sprayed and incubated to the psychrophyte tissue culture seedlings; an adaption environment is created to promote the commensalism of Hirsutella sinensis and psychrophyte tissue during an asexual stage of cordyceps sinensis; the infection rate of the Thitarodes larvae with the cordyceps sinensis material after being subjected to commensalism processing can reach 95.8 to 98.9 percent.

The invention relates to a method for researching co-culture of different types of microorganisms. The method is characterized by respectively sealing a small amount of culture mediums containing different types of microorganisms into dialysis bags with specific apertures in an aseptic environment, soaking the sealed dialysis bags into a liquid culture medium, and culturing. The method can be utilized for simultaneously culturing multiple microorganisms without influencing exchange between various bacteria and nutrient substances and bacterial metabolism substances in the culture medium; after the culture is finished and the bacteria-containing culture mediums in the dialysis bags are taken out in the aseptic environment are subjected to viable count, the growth number of each bacteria can be accurately calculated, and antagonism or commensalism among the different microorganisms can be further researched; besides, by selecting the dialysis bags with different apertures, bacterial proteins entering or exiting dialysis bags can be controlled, and the physiological action of bacterial proteins of different sizes to other bacteria can be researched. The method can be used for simultaneously researching correlation among multiple microorganisms and is simple and rapid.

The invention discloses a method for realizing commensalism of rice and ducks. The method comprises selecting varieties of the rice and the ducks, carrying out soil preparation, sowing, raising the ducks and carrying out foundational construction on a field. The weed and pests in the rice field are eaten by ducklings which have exuberant omnivorousness, and the dung of the ducklings can be used for providing base fertilizer for the rice field, so that organic cultivation of the rice is realized, and the obtained rice is green and pollution-free; after the method is adopted, the effect of obtaining the rice and the ducks at the same time is achieved.

The invention discloses an application of commensalism of transfected mycorrhiza fungi of significant gene and plant rhizosphere. It comprises: transfecting gene good for plant disease resistant, insect resistant and nutrient high- efficiency avalability to bush mycorrhiza fungi to transfe plasmid in said gene onto bush mycorrhiza fungi spore, the bush mycorrhiza fungi will infect the plant root and form commensalism system, and the plant root will obtain resistance to pathogene and growth will be accelerated through significant gene expression. The invention can detect expression effect of bush mycorrhiza fungi under sterile and controllable condition through external triple culturing system (root system of plant, bush mycorrhiza fungi and detection for plant growth index and pathogene in root system of plant).

The invention discloses a method for raising the cotton yield and plantation benefits of high saline and alkaline land. The method comprises the following steps of flattening and desalinating soil in winner; covering the soil with a mulch film in early spring; applying a fertilizer in the last ten-day of April and early May, wherein the fertilizer applied to each hectare of land ranges from 90 kg to 100 kg when being converted into the pure N amount, the P<2>O<5> amount ranges from 42 kg to 45 kg, the K<2>O amount ranges from 30 kg to 35 kg, and a loss-controlled fertilizer mixed with attapulgite is selected as the fertilizer variety; conducting cotton sowing after fertilizer application is ended; after cotton seedlings emerge, conducting seedling final signaling and filling the gaps with seedlings in time, wherein the number of plants per mu is 42-45 thousand plants / hm<2>; during the period when three leaves emerge out of each cotton seedling in early June, intercropping soybeans among cotton lines; harvesting the soybeans in the last ten-day of September, and picking the first batch of ripe unginned cotton. According to the method, by improving the fertilizer application technology and adopting cotton and soybean intercropping, commensalism growth of cotton crops and soybean crops is synergized, fertilizer losses are reduced, and the saline and alkaline land soil is restored. Contrast tests show that compared with a traditional method, by means of the method, the cotton yield and the soybean yield can be obviously raised.

The invention provides a cultivation method of edible fungi rich in superoxide dismutase (SOD). The cultivation method comprises the following steps: in the cultivation process of the edible fungi, applying bacillus cereus capable of producing SOD to performing commensalism with the edible fungi. For the current situation that the SOD content of the edible fungi during the original ecology is toolow, the inventors accidently discover commensalism of the bacillus cereus and the edible fungi can be realized so as to discover that the SOD detected by a pyrogallol autoxidation reduction method isunexpectedly much higher than that of the original state; and the experiment result greatly exceeds the expectation of the ordinary technicians in the field.