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Bacillus megaterium H3 and application thereof in promoting crop growth and reducing absorption of heavy metal by crops

A technology for the growth of Bacillus megaterium and crops, which is applied in the field of agricultural technology and Bacillus application. It can solve the problems that affect the continuous application of passivation repair agents, hinder the absorption of micronutrient elements, and affect the physical and chemical properties of soil, so as to increase production and promote production. The effect of increasing and improving the utilization rate of potassium fertilizer

Active Publication Date: 2017-12-19
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to various inorganic and organic amendments or heavy metals with a certain content (such as phosphate fertilizer, compost, etc.), or will have a significant impact on soil properties and structure (such as lime, clay passivation repair agent), if these are used in large quantities Passivation restoration agent will produce secondary pollution of heavy metals, hinder the absorption of micronutrient elements, affect the physical and chemical properties of soil and other adverse effects, and directly affect the continuous application of passivation restoration agent

Method used

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  • Bacillus megaterium H3 and application thereof in promoting crop growth and reducing absorption of heavy metal by crops
  • Bacillus megaterium H3 and application thereof in promoting crop growth and reducing absorption of heavy metal by crops
  • Bacillus megaterium H3 and application thereof in promoting crop growth and reducing absorption of heavy metal by crops

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The heavy metal-resistant plant growth-promoting bacteria H3 strain (CCTCC NO: M 2016551) of the present invention is isolated and purified from the rhizosphere soil of Pennisetum alopecuroides L. growing in lead-contaminated soil. The isolation and identification method is as follows: the Pennisetum alopecuroides L. Gently pull out the grass from the soil, use sterile tweezers to take the rhizosphere soil that is tightly attached to the root surface of about 2mm of soil, weigh 10g, put it in a triangular flask filled with 90ml of sterile water, shake it fully for 30min, and let it stand 10min, take 0.1ml suspension, adopt 10-fold serial dilution method, spread on broth solid medium (beef extract 3.0g, peptone 5.0g, H 2 O 1000ml, agar 20g, pH 7.0-7.2), culture at 28°C for 3 days, pick a single colony, streak and purify it on the broth solid medium, and save it. The strain was inoculated in broth liquid medium (beef extract 3.0g, peptone 5.0g, H 2 O 1000ml, pH 7.0-7.2),...

Embodiment 2

[0036] Activation of embodiment 2 bacterial strain H3 and preparation of bacterial suspension

[0037]Inoculate the H3 (CCTCC NO: M 2016551) slant bacteria into broth solid medium (beef extract 3.0g, peptone 5.0g, H 2 O 1000ml, agar 20g, pH 7.0-7.2), cultured at 30°C for 3d. Then select plump, viscous T1 bacterium colonies to inoculate in broth liquid medium (beef extract 3.0g, peptone 5.0g, H 2 O 1000ml, pH 7.0-7.2), shake at 150rpm at 30°C for 36h. Transfer the fermentation broth to a sterile centrifuge bottle, centrifuge at 5000 rpm for 5 minutes to collect the bacteria, wash with sterile deionized water, and resuspend to make the number of cells reach more than 500 million CFU / ml.

Embodiment 3

[0038] The ability of embodiment 3 bacterial strain H3 to produce IAA

[0039] According to the determination method of Gordon and Weber (1951), the broth liquid culture medium was divided into test tubes, 4 mL per tube, and 1 mL of 2.5 mg / mL tryptophan solution sterilized by filtration was added after high-temperature sterilization at 121 ° C to make the culture medium The final concentration of tryptophan was 0.5 mg / mL. The H3 (CCTCC NO: M 2016551) bacterial suspension obtained in Example 2 was inoculated into the above-mentioned culture medium, and cultured on a shaker at 30° C. for 3 days. The fermentation broth was centrifuged at 12000r / min for 5min, 1mL of the supernatant was taken, 50μL of 10mM orthophosphoric acid was added, and 2mL of Sackowski's chromogenic reagent was added, mixed thoroughly, the color was developed at 25°C for 30min in the dark, and the absorbance value was measured at a wavelength of 530nm. The sterile medium was treated the same as above and set...

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Abstract

The invention belongs to the fields of agriculture, an agricultural technology and bacillus application, and relates to bacillus megaterium H3 and application thereof in promoting crop growth and reducing absorption of heavy metal by crops. The bacillus megaterium H3 is adopted as a raw material, and is subjected to bacteria activation so as to prepare into a bacterial suspension. The bacillus megaterium H3 bacterial suspension is inoculated in heavy metal contaminated soil, so that the rice growth can be promoted, and the cadmium content in rice grains is reduced. When a passivator and the bacillus megaterium H3 bacterial suspension are complex-used in the heavy metal contaminated soil, the vegetable growth can be promoted, the vegetable quality is improved, and the cadmium content of edible parts of vegetables is reduced.

Description

technical field [0001] The invention belongs to the field of agricultural technology and the application of bacillus, and relates to bacillus megaterium H3 and its application in promoting crop growth and reducing crop absorption of heavy metals. Background technique [0002] With the rapid development of industry and the widespread use of pesticides and chemical fertilizers, cadmium pollution in farmland soil in my country has become increasingly serious. Rice is an important food crop in my country. Heavy metal cadmium accumulates in rice roots, stems, leaves and grains, which not only affects rice yield, quality and the entire farmland ecosystem, but also seriously endangers the health of animals and humans through the food chain. In recent years, farmland contaminated soil remediation technologies represented by passivation (stabilization), phytoremediation, and bioremediation are currently a good choice for the remediation of moderately and lightly polluted soil and he...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P21/00B09C1/10C12R1/11
CPCA01N63/00A01N63/10B09C1/10C12N1/205C12R2001/11
Inventor 盛下放何琳燕王琪李娅夏丽
Owner NANJING AGRICULTURAL UNIVERSITY
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