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Method for preparing antihypertensive peptide by utilizing combined enzyme enzymolysis silkworm chrysalis protein

A blood pressure-lowering peptide and silkworm chrysalis protein technology, applied in the field of bioengineering, can solve the problems of high cost and low yield, and achieve the effects of increasing yield, reducing cost, and improving health care

Active Publication Date: 2010-05-05
常州康和生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is: to provide a method for producing hypotensive peptides by using combined enzymatic hydrolysis of silkworm chrysalis protein, so as to solve the technical problems of low yield and high cost of the original method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Weigh 20 kg of degreased dry silkworm chrysalis powder (moisture 2%, crude protein 67%), add 140 kg of water and grind it into a slurry, after adjusting the pH to 8.0 with 2N NAOH, the temperature of the reaction system is raised to 90 ° C, and after 10 minutes, the After the reaction temperature drops to 60°C, add 60g of alkaline protease (enzyme activity 200000U / g), papain (enzyme activity 800000U / g) 10g, neutral protease (enzyme activity 200000U / g) 10g, keep the temperature and stir constantly The enzymatic hydrolysis reaction was carried out for 240 minutes. After the reaction, the temperature of the reaction system was rapidly raised to 90° C. and kept at the temperature for 10 minutes.

[0016] Use a large-capacity refrigerated centrifuge at 4000r / min to centrifuge at 4°C for 15 minutes, and the supernatant obtained is an enzymatic hydrolyzate containing hypotensive peptide.

[0017] The obtained enzymolyzate was first passed through a dense filter cloth for cent...

Embodiment 2

[0019] Weigh 50kg of fresh complete silkworm pupae (moisture content 10%, crude protein 47%), add 350kg water and grind into a slurry, then add 0.8%-1% NAOH, after the reaction temperature is raised to 60°C, react for 2 hours and keep stirring After the reaction, centrifuge with a 4000r / min centrifuge for 15 minutes, adjust the pH to 4.5 with 2N HCL in the resulting supernatant, keep it at room temperature for 30 minutes and keep stirring, after the reaction, centrifuge for 15 minutes with a 4000r / min centrifuge , the resulting precipitate is silkworm chrysalis protein.

[0020] With 15Kg of gained silkworm chrysalis protein, after adding water 105Kg to dissolve, after adjusting pH to 8.0 with 2N NAOH, reaction system is heated up to 90 DEG C, after keeping 10 minutes, after reaction temperature is down to 60 DEG C, add alkaline protease (enzyme activity 200000U / g) 75g, papain (enzyme activity 800000U / g) 10g, neutral protease (enzyme activity 200000U / g) 10g, keep the temperatu...

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Abstract

The invention relates to the technical field of biological engineering, in particular to a method for preparing antihypertensive peptide by utilizing combined enzyme enzymolysis silkworm chrysalis protein. The method comprises the following steps: dissolving degreased dry silkworm chrysalis powder or silkworm chrysalis protein powder in water in the mass ratio of 1: 7 to 8, adjusting the pH value of the dissolved solution to be 7.5 to 8.5, and then adding combined protease to carry out enzymolysis reaction, wherein the content of alkaline protease is 0.3 to 0.5 percent of the degreased dry silkworm chrysalis powder or the silkworm chrysalis protein powder, the content of papain is 0.05 to 0.1 percent, the content of neutral protease is 0.05 to 0.1 percent, the enzymolysis reaction temperature is 55 to 65 DEG C, and the enzymolysis reaction time is 180 to 240 minutes. The prepared antihypertensive peptide has higher restriction effect on the ACE activity, antihypertensive function on hypertensive patients, does not influence normotensive patients, plays the better health care effect and is safe, has no poison or side effect and is low in cost.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a method for preparing blood pressure-lowering peptides by enzymatically hydrolyzing silkworm chrysalis proteins. Background technique [0002] Angiotensin-converting enzyme (ACE) plays a major role in the regulation of blood pressure. With the continuous deepening of research on food-derived hypotensive peptides, it has been derived from collagen, milk protein, soybean protein, corn protein, fish and shellfish Polypeptides with hypotensive activity are obtained from the enzymatic hydrolysis products or fermentation products of various food-derived proteins such as protein, referred to as hypotensive peptides. At present, the in vitro determination of food-derived polypeptides is mainly to determine the inhibitory activity of polypeptides on ACE enzymes. Peptides with nutritional and physiological activities are extracted from food-derived proteins, which are safe to eat,...

Claims

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Application Information

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IPC IPC(8): C12P21/06
Inventor 袁柏强季卫星
Owner 常州康和生物技术有限公司
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