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Method for producing high-concentration ethanol by fermenting sweet potato raw materials

A high-concentration, raw material technology, applied in the field of microbial fermentation engineering, can solve the problems of amazing starch loss, difficult transportation, high water content, etc., and achieve the effects of reducing production costs, strong operability, and high ethanol concentration

Inactive Publication Date: 2010-11-24
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The raw material of sweet potato has strong seasonality, the harvesting time is relatively concentrated, and the water content is high, so it is very difficult to preserve
After rotting, the viscosity is high, transportation is difficult, and many components that inhibit fermentation will be produced, and the loss of starch is even more alarming.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: Fresh sweet potato is beaten, and water is added according to a certain ratio, so that the total sugar concentration is 270g kg -1 , cook at 80°C for 10 minutes, according to 120KNU kg -1 Add α-amylase to the starch, liquefy at 80°C for 15 minutes, put 100mL of fermented mash in a 250ml Erlenmeyer flask, add 0.5mL of compound pretreatment enzyme solution, inoculate 10mL of yeast seed solution, and add 160AGUkg at the same time -1 Starch glucoamylase, 30°C, shaking 200r / min, fermentation time 36h, ethanol concentration 128.72g kg -1 , fermentation efficiency 90.03%, ethanol production rate 3.58g·kg -1 h -1 .

Embodiment 2

[0034] Embodiment 2: The culture medium is the same as in Embodiment 1, adding 1mL of compound pretreatment enzyme solution, fermentation time 28h, ethanol concentration 132.91g kg -1 , fermentation efficiency 91.44%, ethanol production rate 4.74g·kg -1 h -1 .

Embodiment 3

[0035] Embodiment 3: Fresh sweet potato is beaten, and water is added according to a certain ratio, so that the total sugar concentration is 300g kg-1 , cook at 80°C for 10 minutes, according to 120KNU kg -1 Add α-amylase to the starch, liquefy at 80°C for 15 minutes, put 100mL of fermented mash in a 250ml Erlenmeyer flask, add 1mL of compound pretreatment enzyme solution, inoculate 10mL of yeast seed solution, and add 160AGUkg at the same time -1 Starch glucoamylase, 31°C, shaking 240r / min, fermentation time 39h, ethanol concentration 146.33g kg -1 , the fermentation efficiency is 90.42%, and the ethanol production rate reaches 3.75g·kg -1 h -1 .

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Abstract

The invention belongs to the technical field of microorganism fermentation engineering, and in particular relates to a method for producing high-concentration ethanol by fermenting fresh sweet potato raw materials with slag. The method of the invention comprises three steps, namely fermentation raw material preparation, seed liquor preparation and fermentation. In the process of fermentation raw material preparation, composite pretreatment enzyme solution which contains composite pectinase, cellulose and xylanase is added; and the high-concentration ethanol is obtained after the fermentation. The method has the characteristics of simple process, high operability, high fermentation speed, high final ethanol concentration, high fermentation efficiency, low residual sugar and effective reduction of ethanol production cost.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation engineering, and in particular relates to a method for producing high-concentration ethanol by fermenting fresh sweet potatoes with slag as a raw material. Background technique [0002] With the advent of the energy crisis, the use of renewable resources to ferment and produce ethanol as a bioenergy to replace or partially replace petroleum has been put on the agenda. Fuel ethanol, as a clean energy source, has attracted much attention in today's world of increasing emphasis on environmental protection. At present, due to the low production level, the promotion of fuel ethanol in the market still needs national financial and policy subsidies, but relying on state subsidies is not a long-term solution after all. It is necessary to reduce the production cost of fuel ethanol in order to improve its market competitiveness. [0003] The cost of raw materials accounts for more than half ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/06C12R1/865
CPCY02E50/17Y02E50/10
Inventor 赵海靳艳玲方扬
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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