32 results about "Streptocin" patented technology

The process of extracting lactic streptocin from fermented liquid has one four-stage filtering process including the first stage of sheet frame filtering to eliminate the culture medium grain and great amount of thallus from the fermented liquid, the second stage of micro filtering to eliminate the residual thallus and partial macro molecular hetero protein, the third stage of ultrafiltering with 1,000-5,000 dalton ultrafiltering membrane to eliminate water, inorganic salt, amino acid and small molecular weight organic matter, and the fourth stage of ultrafiltering with 10,000-50,000 dalton ultrafiltering membrane to eliminate hetero protein of molecular weight over 7,000 dalton, pigment and macromolecular impurity, and the subsequent crystallizing and drying to obtain powdered product. The process has the advantages of no pollution, high product quality, low cost, high yield and other advantages.

The invention discloses a Nisin composite biological preservative and a preparation method thereof. The preservative comprises the fermentation broth of lactic streptococci and components consisting of, on the basis of the weight of the fermentation broth of lactic streptococci, 0.2 to 0.6% of chitosan, 0.1 to 0.2% of antioxidant bamboo leaves, 1 to 5% of sodium hexametaphosphate, 0.5 to 1% of natamycin, 0.1 to 0.2% of garlicin, 0.05 to 0.1% of sodium ascorbate, 1 to 3% of maltodextrin and 0.5 to 2% of NaCl. The preservative provided by the invention does not contain any chemical preservative, overcomes the defects of a narrow antibacterial spectrum and low dependence on a pH value of pure Nisin, has the characteristics of high efficiency, a wide spectrum, good stability and high security, greatly improves the preservative effect and the application range of Nisin and produces high economic benefits and social benefits.

The invention discloses a broad-spectrum biological bactericidal air filtration material and a preparation method thereof. The invention is not only applicable to the general families and business departments with the need of air purification, but is also applicable to the semiconductor, pharmaceutical, hospitals and other environments with higher requirements of cleanliness. The broad-spectrum biological bactericidal air filtration material of the invention comprises at least one biological anti-bacterial agent and glass fiber filter paper, and the biological anti-bacterial agent is lysozyme, protamine sulfate, poly-lysine, nisin, polymyxin sulfate, glycine or natamycin. The preparation method of the invention is that the air filtration material is prepared by silanization, hydroformylation, condensation reaction and removal of the physically absorbed anti-bacterial agent. The biological bactericidal air filtration material prepared by the invention has low consumption of the anti-bacterial agent and no influence on the resistance of the filtration material; the sterilization rate is high, most of the killing rate of bacterial, spores and viruses is more than 99 percent, and the fungal growth is inhibited.

The present invention discloses a method for separating and extracting lactic streptocin from fermented liquor. Said method includes the following steps: releasing lactic streptocin, separating and spray drying, under the condition of that temperature is 10-55 deg.C and pressure is 0.5-2.5 MPa adopting inorganic membrane or tubular membrane to filter out thallus and solid matter whose molecular weight is greater than that of lactic streptocin from the fermented liquor, then using rolled membrane ultrafiltration process to remove the matter whose molecular weight is less than that of lactic streptocin so a sto obtain the fermented liquor concentrate.

The invention discloses a compound biological antistaling agent for pork, which is prepared by the following components according to the mass proportions of: 0.3-0.8% of lactoferritin, 0.02-0.05% of lact-streptocin, 0.1-0.5% of lysozyme and balanced amout of water. The invention further discloses a method for keeping and cooling the pork by the biological fresh-keeping agent, which comprises thesteps of: socking the cut cooled pork in the biological fresh-keeping agent for 0.5-1 minute or spraying the biological fresh-keeping agent on the surface of the cut cooled pork; cooling, draining for 3-5 minutes and packaging, storing under the temperature of 0-4 degrees centigrade. The biological fresh-keeping agent of the present invention achieves the advantages of long fresh-keeping time andgood controlled quality, and has nutrition reinforcing and health caring function, but no side-effect to human body. The fresh keeping method in the invention has the advantages of easy operation, low cost, safety, environmental protection and no toxic or side effect.

The invention provides the application of salivaricin in the preparation of medicines for preventing and / or treating autoimmune diseases, and belongs to the technical field of autoimmune diseases treatment. Streptococcus salivarius is an antimicrobial peptide synthesized and secreted by Streptococcus salivarius, which plays an important role in maintaining the local microecological balance of the mucosa. Salivaricin can significantly promote the secretion of anti-inflammatory cytokine IL-10, and inhibit the expression of pro-inflammatory cytokines IL-17, IL-21 and TNF-α. Simultaneously, salivaricin upregulates the proportion of anti-inflammatory regulatory T (Treg) cells, and downregulates the proportion of pro-inflammatory follicular helper T cells and helper T‑17 cells among immune cell subsets. Salivaricin can improve immune imbalance by inhibiting immune response, rebuild immune balance, and provide a new treatment method for autoimmune diseases such as rheumatoid arthritis.

An anticorrosion and fresh-keeping method of packaged sea cucumber products comprises the following steps of: adding an anticorrosion preservative, and sterilizing at the high temperature of 90-120 DEG C for 9-30 min, wherein the anticorrosion preservative is prepared from the following raw materials of: by weight, 0.001-0.05% of sodium erythorbate, 0.01-0.07% of nisin from streptococcus lactis, 0.001-0.005% of potassium sorbate and the balance distilled water. By the adoption of the method for anticorrosion and fresh-keeping processing of packaged sea cucumber products, good quality of the packaged sea cucumber products can be maintained within corresponding shelf-life, and storage period and shelf life are significantly prolonged. In addition, as the added anticorrosion preservative is a commonly-used food additive which accords with GB2760-2011 and has no toxic or side effect, original nutrients and flavor of processed sea cucumber can be maintained. The manufacturing method is simple, requires low cost, has large market potential, and is easy to popularize and use.

The invention relates to an anti-candida-albicans composition containing streptocin and a preparation method thereof and belongs to the technical field of microbial pharmacy. In order to solve the problems that a biomembrane formed by candida albicans blocks drug permeation and reduces the medicinal effect, the anti-candida-albicans composition containing streptocin comprises medicinal componentsincluding streptocin, short collagen peptides, vitamins, menthol, borneol and xylitol. The composition is a viscous gel dressing and can be pasted to an affected mucous part for a long time to enablemore medicinal components to permeate through the biomembrane of candida albicans to take a medicinal effect, and therefore the problem of short acting time of existing dosage forms is solved. Streptocin is uniformly dispersed in the viscous gel dressing in the form of nano-scale liposome and more easily permeate through the biomembrane to directly act on candida albicans thalli, the medicinal effect is more thoroughly exerted, and the effects of reducing the adhesion of candida albicans and inhibiting and killing candida albicans can be rapidly achieved.

The invention relates to a streptocin compound preparation for treating candidiasis of the oral cavity and a preparation method of the preparation and belongs to the technical field of microorganism pharmacy. The problem is solved that streptocin cannot precisely achieve a medicine effect on the infected part of the mucous membrane of the oral cavity. The streptocin compound preparation for treating candidiasis of the oral cavity is a powder injection for injection to the mucous membrane of the oral cavity. An anti-fungal component streptocin is dispersed in the powder injection in a medicine-loading composite microsphere form, the activity and stability of the streptocin are improved, and the bioavailability of the preparation is improved. Flos lonicerae, radix scrophulariae, radix ophiopogonis and menthol can nourish yin, promote the secretion of saliva or body fluid and relieve the symptoms of the dry and scorching hot oval cavity of patients; sodium alga acid and chitosan are safe,free of toxicity, degradable and good in biocompatibility. According to the streptocin compound preparation, the mucous membrane of the injected part is subjected to injection and dosing, the medicine effect can be precisely achieved, the streptocin directly acts on candida albicans, the local concentration is high, and the effects of lowering adhesive power of the candida albicans and inhibitingand killing the candida albicans can be quickly achieved.

The present invention discloses a culture medium for producing lact-streptocin nisin its methods of preparation and method for culturing nisin. The preparation is put yeast protein, soy bean protein into jar, add water and mix, then put carbon, phosphate in turn, mix, debug PH6.2 - 7.2 into jar, add water, and high pressure steam, bactericide, cooling, connect food lactacide, breeding under the condiction of pressure is 0.1 - 0.5 MPa, temperature is 26 - 37 Deg. C for 24 - 72 hours, by biological testing the active of nisin is up to 4000 - 15000 IU / ml, then stop.

The present invention relates to biological antibiotic food additive producing technology, and is especially process of producing. The present invention features that lactic streptocin with plant cake dregs as raw material, and through the steps of enzymolysis, fermentation, solid-liquid separation, concentration, salting out, drying and packing. The technological process of the present invention has greatly raised lactic streptocin yield and lowered production cost.

The invention discloses a method for producing nisin by using cheap raw materials, which belongs to the technical field of microbial fermentation. The present invention uses Streptococcus lactis E-15 that was bred by mutagenesis, uses molasses as a carbon source, and soybean cake or soybean meal as a nitrogen source to ferment under anaerobic conditions, maintains pH 5.0-7.0, and performs batch or fed-batch fermentation 15-30h, the final fermented broth titer reaches 5000IU / mL. The outstanding advantage of the present invention is that cheap molasses and soybean meal are used as raw materials to produce nisin, which reduces the consumption of materials such as sucrose, yeast powder and peptone, and can reduce production costs.