Renewable chromatographic system and method for removing nisin pigment and peculiar smell

Abstract

The invention relates to the technical field of separation of natural food preservatives, in particular to a reproducible chromatographic system and method for removing nisin pigment and peculiar smell. The chromatographic system comprises a liquid inlet system, a high-pressure liquid conveying pump, a mixer, a filtering protection column, a chromatographic column, a detector and a liquid outlet system. Separation is realized on the basis of different acting forces between different components and hydrophobic groups in lactic streptococci fermentation liquor, the lactic streptococci fermentation liquor with low ionic strength is adjusted to be acidic, Nisin with weak adsorption acting force rapidly flows out of a chromatographic column by adopting high-flow-rate power, pigment and odor substances are adsorbed on the surface of a medium, and dissociation is carried out through elution of regeneration liquid. The method provided by the invention overcomes the defects of the traditional process, improves the taste and quality of the food-grade nisin, can realize rapid and efficient separation, has no organic solvent residue, is green and environment-friendly, and has long service life of the chromatographic column and low production cost.

Description

Technical field [0001] The present invention relates to the separation technology of natural food preservatives, and specific relates to regenerative chromatography systems and methods of removing lactic acid-chain bacteria pigment and odor. Background technique [0002] Lactic acid celase (Nisin, Nitern) is also known as lactic acid celase peptide, is a polypeptide compound synthesized and secreted in the metabolic process, consisting of 34 amino acid residues, and the molecular terminal has an amino group and a carboxyl group, amino group. The end is isoleucine, the end of the carboxyl group is lysine, and the molecular weight is approximately 3500 Da. Lactic acid Societium has stable, efficient antibacterial activity, can be absorbed by human body without altering the normal flora in the intestine of the human body, and does not produce drug resistance or cross resistance such as other antibiotics. Lactic acid cemetin can effectively inhibit a variety of Gram-positive bacteria...

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Problems solved by technology

[0004] But on the other hand, there is still the problem of low recovery rate of peptide compounds after separation and purification. Generally, peptides or proteins are easily affected by factors such as temperature, pH value, buffer ionic strength, solvent, etc. during the purification process, resulting in the loss of their own activity.

For example, the patent document CN100554276C establishes the purification of nisin from the culture supernatant of nisin-containing streptococcus nisin. Although the purity and yield are improved, the total recovery rate of the active polypeptide can only reach About 57%, it can be seen that its nisin lost part of its activity in the purification operation

However, the traditional process of removing nisin pigment and odor also has many shortcomings, such as the low activity recovery rate of the foam adsorption method, the solvent extraction method is more polluting to the environment and is prone to solvent residues, and the removal of impurities by the salting out method is incomplete. There are still a lot of miscellaneous proteins and pigments remaining

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