30results about How to "Pigment removal" patented technology

The invention provides a preparation method of longan peel oligosaccharide. The method comprises the steps of selecting materials, drying, pulverizing, degreasing, lixiviating, removing polysaccharide and protein, decoloring, drying and the like. In the preparation method, the oligosaccharide is lixiviated from the longan peel by a scientific process, and the preparation efficiency of the longan peel oligosaccharide is improved on the premise of keeping bioactivity. Macroporous absorbent resin is used for decoloring, which can effectively remove pigment interference. The prepared longan peel oligosaccharide has high yield, obvious anti-oxidative activity and probiotic bacteria proliferation activity, and is applicable to the functional food development field.

The invention discloses a Callicarpa longissima extract, a preparation method and the application thereof, and belongs to the technical field of natural product extraction. A mixture of carnosol, rosmanol and epirosmanol is contained in the Callicarpa longissima extract. The invention also discloses a preparation method of the Callicarpa longissima extract and the application thereof. The mixtureof carnosol, rosmanol and epirosmanol is contained in the Callicarpa longissima extract, and the components are diterpene phenol components respectively, so that the components are of various activities such as preferable oxidation resistance, cancer resistance and anti-inflammation. Through pharmacodynamics study for the Callicarpa longissima extract, a wider space is provided for further application.

The invention discloses a method for simultaneously separating chlorogenic acid and rutin from tobacco leaves. The method includes: weighting 200g of tobacco leave powder sieved through a 60 mesh sieve, dissolving the tobacco leave powder in water according to a material-liquid ratio of 1:20, setting temperature as 50 DEG C, and stirring and extracting the chlorogenic acid and the rutin for 120min. Petroleum ether is used for extraction and removal of oil-soluble impurities for collected filtrate, and then a chlorogenic acid and rutin extracting solution is obtained. Column chromatography isolation of the chlorogenic acid and the rutin is performed on the upper column of the extracting solution. Water of 5 times of the extracting solution in volume is first used for elution until no sugar can be detected in eluate. After the water is used for the elution, different concentration of ethanol replaces the used eluate for gradient elution. Two types of ethanol eluate 10-15% and 30-40% in concentration are respectively collected so as to obtain a chlorogenic acid solution and a rutin solution. The method for simultaneously separating the chlorogenic acid and the rutin from the tobacco leaves saves raw material and reagents, reduces development cost, and achieves good separating effects.

The invention relates to the technical field of building materials, and specifically discloses a high-strength crack-resistant outer wall elastic putty powder, which is prepared by mixing the following raw materials in parts by weight: 600-700 parts of quartz sand, 300-400 parts of gray cement, and putty powder Add 30 to 50 parts of masterbatch; the putty powder masterbatch is composed of the following raw materials in parts by weight: 1.5 to 2.5 parts of palm fiber, 25 to 40 parts of redispersible latex powder, 3 to 3 parts of hydroxypropyl methylcellulose 5 parts, 0.3-0.6 parts of starch ether, 1.5-2.5 parts of polypropylene short fiber, 0.8-1.2 parts of hydrophobic agent, and 0.3-0.6 parts of water reducing agent. At the same time, the invention also discloses a preparation method of the high-strength crack-resistant elastic putty powder for exterior walls. When the putty powder is used to paint exterior walls, it can obviously improve the crack resistance of the wall coating. The bonding force of the base layer is stronger and it is not easy to fall off.

The invention discloses a method used for high-efficient purification of pneumocandins B0. The method comprises following steps: (1) pH value of a fermentation broth containing pneumocandins B0 is adjusted to 2.0 to 4.0, the fermentation broth is filtered, and a pneumocandins B0 extract liquid is obtained via extraction; (2) the extract liquid is condensed, diatomite is added for crystal packaging, water is added, and an obtained mixture is stirred and is subjected to centrifugation; (3) a solid material obtained via centrifugation is dissolved in ethanol, active carbon is added for decolouring, and an obtained mixed material is filtered; (4) an obtained filtrate is condensed, chloroform is added, an obtained solution is delivered through a silica gel column, and a pneumocandins B0 eluant is collected; and (5) the pneumocandins B0 eluant is condensed to be dry, and crystallization is carried out in a multiphase solvent system so as to obtain pneumocandins B0. The method is capable of avoiding application of resin columns and reducing amount of solvents used for elution greatly; active carbon can be used for removing pigments effectively; silica gel column chromatography is capable of removing related substances C0; and extraction purity can be as high as 99%. The method is simple and convenient; cost is reduced greatly; and industrialized production can be easily realized.

The invention discloses a Dendrobium officinale leaf polysaccharide and its preparation and application. The preparation method is carried out in sequence according to the following steps: (1) washing, drying and pulverizing the Dendrobium officinale leaves; (2) soaking the processed material in an aqueous ethanol solution with a volume concentration of 60%-95% to extract to Remove impurities, wherein the extraction times are 2-6 times, and the concentration of the aqueous ethanolic solution used in the latter extraction is higher than the concentration of the aqueous ethanolic solution used in the previous extraction, so as to obtain the soaked sample; (3) the soaked sample is extracted with water to obtain a water extract; ( 4) enzymolysis with protease; (5) decolorization with activated carbon; (6) concentrating the supernatant, adding an aqueous ethanol solution and leaving it to stand overnight, and centrifuging to obtain a precipitate; (7) dissolving the precipitate in distilled water, and freeze-drying to obtain Dendrobium officinale leaves polysaccharides. The dendrobium leaf polysaccharide prepared by the invention has the effect of improving immune deficiency and restoring normal health, and can be used for preparing food or health care product with the effect of improving immune deficiency.

The invention discloses a third-generation sequencing zooplankton genome DNA extraction method and application. The method comprises the following steps: (1) adding 18% to 20% (V / V) β-mercaptoethanol to buffer GA, and then adding zooplankton for grinding to obtain tissue buffer; (2) adding Add protease PK for digestion, then add RNase A, let stand, centrifuge, and take the supernatant; then add solvent, centrifuge, and take the supernatant; finally add buffer GB, mix well and add absolute ethanol to get the mixture; (3) Add the mixed solution to the adsorption column CB3, perform rinsing and elution, and obtain the zooplankton genomic DNA. The method of the present invention is simple, easy to operate, and can effectively remove pigments, and the obtained genomic DNA fragments of zooplankton are complete, high in purity, and high in concentration, which provides a guarantee for the smooth development of the third-generation sequencing of zooplankton, especially cladocera .