Extraction and purification method of 10-deacetylbaccatin III

Abstract

The invention discloses an extraction and purification method of 10-deacetylbaccatin III, which comprises the following steps of treating taxus chinensis branches and leaves, extracting, filtering, concentrating, extracting, washing and concentrating to obtain a crude product A, carrying out freeze suction filtration on the crude product A to obtain a crude product B, and concentrating, freezing, filtering and washing the crude product B to obtain a crude product C, concentrating, freezing and filtering the crude product C to obtain a final extracted and purified 10-deacetylbaccatin III product. After taxus chinensis branches and leaves are extracted, a sodium bicarbonate aqueous solution is used for washing to remove acidic impurities and pigments in the material, so that the content of 10-deacetylbaccatin III in the extract concentrate is greater than or equal to 10%, the standard of acetonitrile washing can be achieved, a chromatographic separation step is omitted in the whole process, the production efficiency is improved, the production time is shortened, and after the crude product B is crystallized, the crude product B is washed with an acetone-n-hexane mixed solvent to remove pigment in the crude product B crystal. The content of the finally obtained 10-deacetylbaccatin III is as high as 99.23%, the purity reaches 99.37%, and the product yield reaches 75.99%.

Description

technical field [0001] The invention relates to the technical field of compound separation and purification, in particular to a method for extracting and purifying 10-deacetylbaccatin III from branches and leaves of Taxus chinensis. Background technique [0002] Paclitaxel and docetaxel are currently important taxane anticancer drugs, which are used for the treatment of advanced or metastatic breast cancer that failed prior chemotherapy, advanced or metastatic non-small cell lung cancer that failed cisplatin-based chemotherapy, combined with a Cyclophosphamide is used for the treatment of operable, node-positive breast cancer, combined with cisplatin for the first-line treatment of locally advanced or metastatic non-small cell lung cancer, combined with prednisone for the treatment of androgen-independent metastatic prostate cancer, combined with cisplatin Platinum and 5-fluorouracil first-line treatment of advanced gastric cancer. Paclitaxel naturally exists in Taxus chine...

AI Technical Summary

Problems solved by technology

[0005] The deficiencies of the existing 10-deacetylbaccatin III industrialized production mainly include that in the purification process, most of the processes adopt silica gel chromatography separation and resin adsorption separation, and these two production processes take a long time, The production efficiency is low, leading to an increase in product costs, which squeezes out the company's due profits