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Preparation of longan peel oligosaccharides

A technology of oligosaccharides and longan, applied in the direction of oligosaccharides, etc., to achieve the effects of improving the purity and yield, reducing the loss of oligosaccharides, good antioxidant activity and probiotic proliferation activity

Inactive Publication Date: 2009-06-24
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on the deep development of longan peel, and there is no report on the preparation of oligosaccharides from longan peel

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Prepare longan peel oligosaccharides as follows.

[0019] 1) Material selection: choose fresh longan, the surface of the peel has no obvious rot or disease phenomenon, remove the pulp and stone, and collect the longan peel;

[0020] 2) drying and pulverizing: drying the collected longan peel, using a pulverizer to pulverize into powder, and passing through a 100-mesh sieve to obtain longan peel powder;

[0021] 3) Degreasing: adding 5 liters of petroleum ether to 1 kg of longan peel powder, reflux extraction at 40°C for 2 hours, and collecting the solid residue to obtain defatted longan peel powder;

[0022] 4) Extraction: add defatted longan peel powder to 4 liters of distilled water, extract at 50°C for 3 hours, and filter to remove residue;

[0023] 5) Removal of polysaccharides and proteins: Concentrate the filtrate at 60°C to 1 / 3 of the original volume, add ethanol to a concentration of 80% by volume, leave it overnight, centrifuge to remove the precipitate, and c...

Embodiment 2

[0028] Prepare longan peel oligosaccharides as follows.

[0029] 1) Material selection: choose fresh longan, the surface of the peel has no obvious rot or disease phenomenon, remove the pulp and stone, and collect the longan peel;

[0030] 2) drying and pulverizing: drying the collected longan peel, using a pulverizer to pulverize into powder, and passing through a 120-mesh sieve to obtain longan peel powder;

[0031] 3) Degreasing: adding 5 liters of petroleum ether to 1 kg of longan peel powder, reflux extraction at 60°C for 2 hours, and collecting the solid residue to obtain defatted longan peel powder;

[0032] 4) Extraction: add defatted longan peel powder to 5 liters of distilled water, extract at 80°C for 5 hours, filter, and collect the filtrate;

[0033] 5) Removal of polysaccharides and proteins: Concentrate the above filtrate at 80°C to 1 / 3 of the original volume, add ethanol until the volume ratio concentration is 75%, leave it overnight, centrifuge to remove the ...

Embodiment 3

[0038] Prepare longan peel oligosaccharides as follows.

[0039] 1) Material selection: choose fresh longan, the surface of the peel has no obvious rot or disease phenomenon, remove the pulp and stone, and collect the longan peel;

[0040] 2) Drying and crushing: drying the collected longan peel at 50°C, pulverizing it into powder with a traditional Chinese medicine grinder, and passing through a 60-mesh sieve to obtain longan peel powder;

[0041] 3) Degreasing: Add 4 liters of petroleum ether to 1 kg of longan peel powder, reflux and extract at 50°C for 1.5 hours, collect the solid residue to obtain defatted longan peel powder;

[0042] 4) Extraction: add defatted longan peel powder to 3 liters of distilled water, extract at 80°C for 4 hours, filter, and collect the filtrate;

[0043] 5) Removal of polysaccharides and proteins: Concentrate the above filtrate at 65°C to 1 / 3 of the original volume, add ethanol until the volume ratio concentration is 85%, leave it overnight, c...

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PUM

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Abstract

The invention provides a preparation method of longan peel oligosaccharide. The method comprises the steps of selecting materials, drying, pulverizing, degreasing, lixiviating, removing polysaccharide and protein, decoloring, drying and the like. In the preparation method, the oligosaccharide is lixiviated from the longan peel by a scientific process, and the preparation efficiency of the longan peel oligosaccharide is improved on the premise of keeping bioactivity. Macroporous absorbent resin is used for decoloring, which can effectively remove pigment interference. The prepared longan peel oligosaccharide has high yield, obvious anti-oxidative activity and probiotic bacteria proliferation activity, and is applicable to the functional food development field.

Description

technical field [0001] The invention relates to a method for preparing oligosaccharides from fruit peels, in particular to a method for preparing oligosaccharides from longan peels. Background technique [0002] Longan belongs to the Sapindaceae longan genus and belongs to one of the four famous fruits in south of the Five Ridges. It has been cultivated for more than two thousand years so far. Fujian, Guangxi, Guangdong, Taiwan, Hainan, Sichuan, Chongqing, Yunnan and Guizhou have a large number of plantings. In 2005, my country's longan planting area was 460,000 hectares, with an output of 940,000 tons, accounting for 73.6% of the world's total planting area and 59.7% of the total output. Longan pericarp accounts for about 18% of the total weight of the fruit, and is an important resource of natural active substances. "Xian Xing Zhai Medical Wide Notes" once recorded that the longan peel "has the power of dispersing wind and soothing the exterior, cooling blood and heat". ...

Claims

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Application Information

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IPC IPC(8): C07H3/06C08B37/00
Inventor 杨宝蒋国祥蒋跃明
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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