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Method for producing oligopeptide by using silkworm cocoon degumming solution and waste silkworm cocoons as raw materials

An oligopeptide and silkworm cocoon technology, applied in the field of biotechnology, can solve the problems of no molecular weight determination of enzymatic hydrolysis products, no systematic screening of enzymes for enzymatic hydrolysis, and high production costs, so as to improve product color, facilitate digestion and absorption, and reduce equipment requirements. and environmental effects

Inactive Publication Date: 2017-06-30
大连平岛天然产物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] (3) Sericin and silk fibroin are both proteins, and their amino acids are similar or similar. All processes are light desericin, and then the silk fibroin is dissolved and then enzymatically hydrolyzed. The process is long and the production cost is high.
[0009] (4) There is no systematic screening of enzymes for enzymatic hydrolysis, so it is not known which enzyme or which enzymes are most suitable for the dissolution of silkworm cocoon protein. Many documents mention the use of alkaline protease, and practice has proved that the product of alkaline protease enzymatic hydrolysis has a bitter taste heavy, bad taste
[0010] (5) Most of the literature does not measure the molecular weight of the enzymatic hydrolysis product

Method used

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  • Method for producing oligopeptide by using silkworm cocoon degumming solution and waste silkworm cocoons as raw materials

Examples

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preparation example Construction

[0030] Step 2. Preparation of silk fibroin protein liquid: cut the waste silkworm cocoon and remove the cocoon fragments, filter and dehydrate the excrement of the pupae in the cocoon in an industrial washing machine to obtain a clean silkworm cocoon, put the clean silkworm cocoon into the boiling concentration of 0.8% Stir and dissolve in -1.5% NaOH solution for 1-3 hours, cool down the solution properly, and centrifuge. If there is any precipitate, it can be heated and dissolved with the same concentration of lye, and the solution is combined into a silk protein solution; after the silk protein solution is filtered with a ceramic membrane , through an alkaline organic membrane with a molecular weight cut-off of 0.1ku-5ku, the permeate is lye, which can be reused for dissolving the next batch of cocoon shells, and the retentate is silk fibroin liquid, which can be enzymatically hydrolyzed alone. Or the silk fibroin solution can also be enzymatically hydrolyzed together with th...

Embodiment 1

[0034] Example 1: The waste silkworm cocoon and degumming solution used in the research of this example were provided by a silk reeling factory in Anhui, Anhui Province. It was verified that the protein content of the waste silkworm cocoon shell was 98.17%, and the protein content of the degumming solution was 2.69%.

[0035] The method for producing oligopeptides in this embodiment is: the degumming solution is filtered through a ceramic membrane to obtain a clear solution, and the clear solution is filtered through a 10ku organic membrane, and the permeate is a degumming solution without gelatin, which can be repeatedly used for degumming. The intercepted liquid is sericin, add 3-4 times of pure water to dilute, wash the degummed liquid in the sericin as much as possible, make the pH of the sericin liquid around 8.0, take the sericin liquid, adjust it with pure water The substrate concentration is about 5%; warm the feed solution to 47°C±1°C, use NaOH solution to season the p...

Embodiment 2

[0036] Example 2: The waste silkworm cocoon and degumming solution used in the research of this example were provided by a silk reeling factory in Anhui, Anhui Province. It was verified that the protein content of the waste silkworm cocoon shell was 98.17%, and the protein content of the degumming solution was 2.69%.

[0037] The method for producing oligopeptides in this example is: add the washed silkworm cocoon fragments into a boiling 1.1% NaOH solution, stir and dissolve for 2 hours, centrifuge the silkworm cocoon solution to obtain a liquid phase, and add an appropriate amount of 1.1% NaOH if there is a small amount of precipitation The solution is heated to dissolve, and after centrifugation, it is merged into the liquid phase of the first centrifugation, and the liquid phase passes through the ceramic membrane to obtain the filtrate. The clear liquid is filtered with an alkaline organic membrane with a molecular weight cut-off of 0.5ku, and the permeate is lye, which ca...

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Abstract

The invention discloses a method for producing oligopeptide by using a silkworm cocoon degumming solution and waste silkworm cocoons as raw materials, and relates to the technical field of biotechnology. The method for producing oligopeptide by using the silkworm cocoon degumming solution and the waste silkworm cocoons comprises the following steps: step one, preparation of a sericin solution; step two, preparation of a silk fibroin solution; step three, mixed enzymolysis of the silk fibroin solution and the sericin solution; step four, decarburization evaporation and concentration of an enzymatic hydrolysate. A permeate liquid can be repeatedly used, so that the cost is reduced, the emission is reduced, and the product color and luster are improved. Clean cocoon shells are heated and dissolved with dilute alkaline, and the using amount of the alkaline is greatly lower than a concentration reported in the literature, so that the production cost can be reduced, and the requirements for equipment, environmental protection and labor protection are reduced. A small quantity of undissolved cocoon silk can be dissolved with the same alkaline liquor again, so that the raw materials are sufficiently utilized. Directionally filtered compound protease is adopted in silk-fibroin liquid enzymolysis, the enzymolysis yield reaches 90%, the product molecular weight is small, the digestive absorption is facilitated, and the product taste is good.

Description

[0001] Technical field: [0002] The invention relates to a method for producing oligopeptides by using silkworm cocoon degumming liquid and waste silkworm cocoons as raw materials, and belongs to the technical field of biotechnology. [0003] Background technique: [0004] Mulberry silk fiber is one of the earliest natural proteins used by humans. The characteristics of its fabric are well known. At the same time, as the object of basic research, it has a series of advantages such as high purity and wide sources. Mulberry silk is mainly composed of silk fibroin and sericin. Silk fibroin is the main component of silk, accounting for about 70% of the total weight. Based on the antiparallel folding (β-sheet) conformation, silk fibroin molecules form microfibers with a diameter of about 10 nm. 1 μm thin fiber, about 100 thin fibers are arranged along the long axis to form a single fiber with a diameter of 10-18 μm, this single fiber is silk protein fiber. Due to the special stru...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435C12P21/06C07K1/34C07K1/14
CPCC07K14/43586C12P21/06
Inventor 黄磊宋志凤刘心雨
Owner 大连平岛天然产物科技有限公司
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