Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for simultaneously extracting and separating chlorogenic acid and rutin from tobacco leaves

A technology of chlorogenic acid and rutin, applied in chemical instruments and methods, separation/purification of carboxylate, preparation of carboxylate, etc., to achieve the effects of simple separation, reduction of pigment formation, and simple operation

Active Publication Date: 2019-07-12
CHINA TOBACCO CENTRAL SOUTH AGRICULTURAL EXPERIMENTAL STATION +1
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, we have not seen the process report on the simultaneous separation of chlorogenic acid and rutin on the basis of sugar and pigment removal

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for simultaneously extracting and separating chlorogenic acid and rutin from tobacco leaves
  • A method for simultaneously extracting and separating chlorogenic acid and rutin from tobacco leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Simultaneous extraction of tobacco chlorogenic acid and rutin

[0031] Weigh 200 g of tobacco leaf powder sample that has passed through a 60-mesh sieve, dissolve it in 4000 ml of water at a solid-liquid ratio of 1:20, pour it into a stirring extractor, set the extraction temperature to 50 °C, and perform the stirring and extraction time for 120 min for extraction. The obtained extract was subjected to suction filtration, the residue was filtered off, and the filtrate was collected. The extraction rates of tobacco chlorogenic acid and rutin in the filtrate reached 84% and 99% respectively (according to the experimental data of recovery rate). The contents of chlorogenic acid and rutin in the filtrate reached 21.7 mg / g and 16.5 mg / g, respectively. The filtrate was extracted with petroleum ether at a volume ratio of 1:1 to remove impurities, the upper organic phase was recovered, and the lower aqueous phase was combined to obtain the chlorogenic acid and rutin extract...

Embodiment 2

[0039] 1. Simultaneous extraction of tobacco chlorogenic acid and rutin

[0040] Weigh 200 g of tobacco leaf powder sample that has passed through a 60-mesh sieve, dissolve it in 5000 ml of water at a material-to-liquid ratio of 1:25, pour it into a stirring extractor, set the extraction temperature to 50 °C, and perform the stirring and extraction time for 120 min for extraction. The obtained extract was subjected to suction filtration, the residue was filtered off, and the filtrate was collected. The filtrate is extracted with petroleum ether to remove impurities according to the volume ratio of 1:1, the upper organic phase is recovered, and the lower aqueous phase is combined to obtain the chlorogenic acid and rutin extract.

[0041] 2. Separation of Tobacco Chlorogenic Acid and Rutin

[0042] 1. Isolation of chlorogenic acid

[0043] Load the pretreated HPD-100 macroporous resin into the chromatography column, so that the column surface is flat, without tilt, without air...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for simultaneously separating chlorogenic acid and rutin from tobacco leaves. The method includes: weighting 200g of tobacco leave powder sieved through a 60 mesh sieve, dissolving the tobacco leave powder in water according to a material-liquid ratio of 1:20, setting temperature as 50 DEG C, and stirring and extracting the chlorogenic acid and the rutin for 120min. Petroleum ether is used for extraction and removal of oil-soluble impurities for collected filtrate, and then a chlorogenic acid and rutin extracting solution is obtained. Column chromatography isolation of the chlorogenic acid and the rutin is performed on the upper column of the extracting solution. Water of 5 times of the extracting solution in volume is first used for elution until no sugar can be detected in eluate. After the water is used for the elution, different concentration of ethanol replaces the used eluate for gradient elution. Two types of ethanol eluate 10-15% and 30-40% in concentration are respectively collected so as to obtain a chlorogenic acid solution and a rutin solution. The method for simultaneously separating the chlorogenic acid and the rutin from the tobacco leaves saves raw material and reagents, reduces development cost, and achieves good separating effects.

Description

technical field [0001] The invention belongs to the technical field of plant active ingredient extraction, and relates to a method for simultaneously extracting and separating tobacco chlorogenic acid and rutin from tobacco leaves. Background technique [0002] my country's tobacco planting area and output rank first in the world, with an annual production of more than 2 million tons of tobacco leaves. According to statistics, more than 700 billion yuan of profits and taxes were paid to the state in 2013. Therefore, tobacco production and cigarette processing occupy a very important position in my country's national economy. However, in recent years, affected by global climate change, frequent natural disasters and the "Framework Convention on Tobacco Control" advocated by the World Health Organization, tobacco companies have experienced excessive storage of tobacco leaves, and about 30% of low-grade tobacco leaves cannot be used for cigarette production. According to stati...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07C67/48C07C67/56C07C67/58C07C69/732C07H1/08C07H17/07
CPCC07C67/48C07C67/56C07C67/58C07H1/08C07H17/07C07C69/732
Inventor 彭曙光王征许诗豪杨红旗杨红武
Owner CHINA TOBACCO CENTRAL SOUTH AGRICULTURAL EXPERIMENTAL STATION
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com