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Method for preparing stevioside and chlorogenic acid from stevia step by step

A technology of chlorogenic acid and stevioside, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problem of high cost of solvent and solvent removal, achieve high utilization rate of raw materials and equipment, and high economy The effect of high benefit and high yield

Active Publication Date: 2013-02-13
CHENGUANG BIOTECH GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN 102617667 A discloses a method for simultaneously preparing total caffeoylquinic acid and stevioside using stevia as raw material, which uses a solvent extraction method for liquid-liquid separation of stevioside and total caffeoquinic acid; this method is applied to various Solvent, solvent and solvent removal costs are high, and there are trace residues in the product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: The method for preparing stevioside and chlorogenic acid step by step from stevia leaves adopts the following process steps.

[0021] 100kg of stevia leaves are soaked in 2°C water to obtain 1510m of extract 3 ; Add 3kg / m to the extract 3 Add ferrous sulfate and calcium oxide to adjust the pH of the solution to 8.1, stir evenly for flocculation, and filter the flocculation liquid to obtain filtrate and filter cake. The filtrate was adsorbed with DM30 macroporous adsorption resin; the adsorbed resin was analyzed with ethanol with a mass concentration of 81% to obtain a stevioside analysis solution; the analysis solution was desalted, decolorized, concentrated and dried to obtain 9.2 kg of a stevioside product with a content of 92.5 wt%. The filter cake is dissolved in river water, adjusted to pH=0.5 with acid, and filtered to obtain chlorogenic acid filtrate; the chlorogenic acid filtrate is adsorbed with macroporous adsorption resin NKA-9, then analyzed ...

Embodiment 2

[0022] Embodiment 2: The method for preparing stevioside and chlorogenic acid step by step from stevia leaves adopts the following process steps.

[0023] 100kg of stevia leaves were soaked in 9°C water to obtain an extract of 1460m 3 ; Add 7kg / m to the extract 3 Ferrous chloride and calcium oxide are added to adjust the pH of the solution to 9.9 and stirred evenly for flocculation, and the flocculation liquid is filtered to obtain filtrate and filter cake. The filtrate was adsorbed with DM30 macroporous adsorption resin; the resin was analyzed with ethanol with a mass concentration of 76% to obtain a stevioside analysis solution, which was refined to obtain 8.9 kg of a stevioside product with a content of 93.1 wt%. Dissolve the filter cake in well water, adjust the pH value to 5.5 with acid, and filter to obtain chlorogenic acid filtrate; the filtrate is adsorbed with macroporous adsorption resin NKA-9, analyzed with ethanol with a mass concentration of 86%, concentrated an...

Embodiment 3

[0024] Embodiment 3: The method for preparing stevioside and chlorogenic acid step by step from stevia leaves adopts the following process steps.

[0025] 100kg of stevia leaves are soaked in 5°C water to obtain an extract of 1480m 3 ;Add 5kg / m to the extract 3 Ferrous ammonium sulfate and calcium oxide are added to adjust the solution to pH 9.1 and stirred evenly for flocculation; the flocculation liquid is filtered to obtain filtrate and filter cake; the filtrate is adsorbed on DM30 macroporous adsorption resin, and the lower column water is collected. Resin is decomposed with mass concentration 78% methanol to get stevioside analysis solution, and analysis solution obtains 9.1kg of stevioside product of content 92.3wt% through refining treatment; Filter cake is adjusted pH value 3.4 with acid after being dissolved in lower column water, and filter Chlorogenic acid filtrate; the filtrate is adsorbed with macroporous adsorption resin NKA-9, analyzed with methanol with a mass...

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Abstract

The invention discloses a method for preparing stevioside and chlorogenic acid from stevia step by step. The method comprises the following steps of: (1) soaking the stevia with cold water and extracting to obtain extract; (2) adding a bivalent iron salt into the extract to regulate pH value, flocculating, and filtering the flocculation solution to obtain filtrate and a filter cake; (3) adsorbing the filtrate by using macroporous adsorption resin, desorbing and refining to obtain the stevioside; (4) dissolving the filter cakes with water, regulating the pH value to be 0.5-5.5 by using acid, and filtering to obtain the chlorogenic acid filtrate; and (5) adsorbing the chlorogenic acid filtrate by using macroporous adsorption resin, desorbing, concentrating, drying, and re-crystallizing, thus obtaining chlorogenic acid. According to the method, the stevioside and the chlorogenic acid are extracted at the same time, so that a product is added, and the extraction cost of the stevioside is reduced; and the method is easy to operate, low in cost, high in chlorogenic acid yield, water-saving, environment-friendly, simple in equipment, high in utilization rate of raw materials and equipment and suitable for industrialized production.

Description

technical field [0001] The invention relates to a method for extracting natural extracts, in particular to a method for step-by-step preparation of stevioside and chlorogenic acid from stevia leaves. Background technique [0002] Stevioside (glycoside) is a new type of natural sweetener extracted from stevia leaves of Compositae herb, which has the characteristics of high sweetness and low calorie. [0003] Chlorogenic acid (1,3,4,5-tetrahydroxycyclohexanecarboxylic acid-(3,4-dihydroxycinnamate), also known as: chlorogenic acid, coffee tannic acid, 3-caffeoylquinic acid) Belongs to caffeoylquinic acid derivatives, has strong antioxidant capacity, and also has anti-HIV, tumor cell line toxicity, anti-teratogenic, anti-allergic and regulates the activity of enzymes linked by cytochrome P450. Chlorogenic acid is an important ingredient in virus medicine and other medicines, and it is widely used. Chlorogenic acid is known as "plant gold" and is the target of many manufacturer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/256C07H1/08C07C69/732C07C67/48
Inventor 田洪丁红梅连运河牛志平崔柳
Owner CHENGUANG BIOTECH GRP CO LTD
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