The invention relates to a preparation method of a surface
molecularly imprinted polymer of PN (
pneumocandins)B0. The preparation method comprises the following steps: step 1, SiO2 is taken and placed in a container filled with a strong acid solution, reacts, and is left to stand, washed to neutral with
distilled water and dried; step 2, gamma-trimethoxysilyl propyl
methacrylate,
ethanol and water are added to the substance obtained in the step 1, the mixture reacts, and a product is dried; step 3, water and
methacrylic acid are added to the substance obtained in the step 2, N2 is adopted to exhaust air, the temperature is increased, an initiator
ammonium persulfate is added, the mixture reacts, and a product is dried; step 4, PNB0,
ethanol and
ethylene glycol
diglycidyl ether are added to the substance obtained in the step 3, pH is regulated, the mixture reacts, PNB0 is removed with a polar
solvent, a product is dried, and the PNB0 molecularly imprinted material is prepared. The preparation method adopts a surface
molecular imprinting technology and overcomes the defect of complex PNB0 extraction and purification processes in the prior art, the purity of PNB0 is improved, the content of PNB0 is increased, and industrial production of PNB0 is simpler and more convenient.