Method of purifying pneumocandins B0

Abstract

The invention discloses a purification method of pneumocandins B0 through process optimization. The method comprises the following steps: (1) acidifying fermenting liquor of pneumocandins B0, filtering the fermenting liquor and performing extraction to obtain extraction liquor; (2) adding diatomite into an extraction liquor concentrated mixture to wrap the crystals, and adding deionized water to centrifugalize so as to obtain a white solid containing pneumocandins B0; (3) dissolving the centrifugalized solid by adding ethanol, fully stirring the mixture, then adding activated carbon, fully stirring the mixture to decolor, and performing filtration to obtain a colorless filtrate; (4) concentrating the colorless filtrate till the mixture is dried, adding a heterogeneous solvent system, dissolving, and performing devitrification to obtain humid crystals; and (5) further adding the obtained humid crystals into the heterogeneous solvent system, dissolving, and performing devitrification to obtain the humid crystals and drying the same. On the basis of keeping the purity unchanged, the method not only simplifies the process steps, but also reduces the production cost, so that industrial scaled production is further facilitated.

Description

technical field [0001] The invention belongs to the field of medicinal chemistry and relates to pneumocantine B 0 The industrialized production of, be specifically related to a kind of purification pneumocidine B 0 industrial methods. Background technique [0002] Neomercontin B 0 It is a secondary metabolite synthesized by the fermentation of the fungus Glarea lozoyensis. In addition to the target product, the fermentation product of Glarealozoyensis has C 0 and other components, as well as other impurities such as a large amount of pigment, so Newmocontin B 0 The extraction and purification process is difficult. [0003] [0004] Chinese invention patent 200910133118.0 discloses the preparation of pneumocandine B 0 The method, the main steps are: a) centrifugal nemocontin B 0 Fermentation broth, take the mycelia, and extract neomocontin B with methanol 0 ; b) the normethanol extract is evaporated to dryness, and then leached with n-butanol to pneumocondine B 0 ...

AI Technical Summary

Problems solved by technology

This method was developed by Borui Biopharmaceutical Taixing Co., Ltd., a subsidiary of the parent company of the applicant company. However, during the process handover, the inventor found that although this method can effectively control C 0 content, making pneumocantine B 0 The purity can reach more than 99%, but in the whole process, column chromatography is required. When column chromatography is applied to industrial production, it is time-consuming, the amount of solvent used is large, it is easy to cause environmental pollution, and the loss of raw materials is large, which greatly increases the production cost.

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