High-yield strain for pneumocandin B0 and application for same

A technology of neumocantine and high-yielding strains, which is applied in the field of bioengineering, can solve the problems of complex fermentation components, reduced drug efficacy, large toxic and side effects, etc., and achieves the effects of stable genetic traits and high industrialization value.

Inactive Publication Date: 2013-09-11
SHANGHAI LAIYI BIOMEDICAL RES & DEV CENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] First, because the targets of these two types of drugs are fungal cell membranes, their selectivity is poor, resulting in relatively large toxic and side effects;
[0005] The second is the reduced efficacy of these drugs due to the widespread emergence of clinically resistant fungi;
[0006] The third is that these drugs are not very sensitive to fungi such as Aspergillus and Candida albicans, which makes it difficult to control the disease of this type of fungal infection clinically.
Because the fermentation yield of pneumocidine B0 is very low, and the fermentation components are complex, which in turn affects the yield of caspofungin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 strain stability investigation

[0034] Medium:

[0035] Slant / Plate Medium: Potato Dextrose Agar Medium.

[0036] Seed Medium (%): Glucose 2.5, KH 2 PO 4 0.9, Yeast powder 0.5, Medicinal agent (Cottonseed powder, Cotton Purple, the same below) 1.0, 85% lactic acid 0.2ml, trace elements 0.1ml, pH 6.0

[0037] Fermentation medium (%): sucrose 12.5, sodium glutamate 0.8, yeast powder 0.8, proline 1.5, KH 2 PO 4 0.15, magnesium sulfate heptahydrate 0.04, trace elements 1ml, MES buffer salt 1.5, pH 5.3.

[0038] Trace element composition (g / L): ferrous sulfate heptahydrate 1.0, manganese sulfate monohydrate 0.2, calcium chloride dihydrate 0.1, boric acid 0.056, copper chloride dihydrate 0.025, ammonium molybdate tetrahydrate 0.019, 12N hydrochloric acid 50ml.

[0039] Training conditions:

[0040] Incline cultivation temperature is 28°C, cultivation time is 5 days;

[0041] The cultivation temperature of the seed liquid is 25°C, and it is cultivated f...

Embodiment 2

[0060] Embodiment 2 mutant strain compares with starting strain

[0061] Medium, culture condition and detection method, as embodiment 2.

[0062] The comparison between the mutant strain and the starting strain mainly takes the B0 content of pneumocidine as an example, and the comparison results are shown in Table 3:

[0063] Table 3 Comparison of mutant strains and starting strains

[0064] bacteria Starting strain mutant strain Nimocontin B0 content (mg / l) 232 4537 Relative amount 100％ 1955％ relative increase —— 18.5 times

[0065] It can be seen from Table 3 that the content of pneumocantine B0 in the mutant strain prepared by the present invention is 18.5 times higher than that of the starting strain.

[0066] By fermenting the high-yielding bacterial strain provided by the present invention, pneumocandine B0 can be obtained in high yield, thereby ensuring the yield of caspofungin and reducing the cost of caspofungin.

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Abstract

The invention discloses a high-yield strain for pneumocandin B0, which is the mutant strain of Zalerion arboricola ATCC46001 strain, and collected in the China General Microbiological Culture Collection Centre with a collection number of CGMCC No. 5412. The invention further discloses an application for the same, wherein the yield of pneumocandin B0 prepared from the obtained high-yield strain for pneumocandin B0 achieves about 4500 mg/l.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, in particular, it relates to a high-yield pneumocidine B0 strain and its application. Background technique [0002] In the past two decades, fungal infections that seriously endanger human life and health have been increasing in terms of mortality and types of infections, especially in immunosuppressed patients. The main drugs currently used to treat clinical deep fungal infections are azole antifungal drugs and amphotericin B. [0003] Although these two types of drugs have played an important role in controlling clinical deep fungal infections, the mortality rate of deep fungal infections remains high due to the following three deficiencies of these drugs: [0004] First, because the targets of these two types of drugs are fungal cell membranes, their selectivity is poor, resulting in relatively large toxic and side effects; [0005] The second is the reduced efficacy of these drugs du...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P21/04C12R1/645
Inventor 罗敏玉孙新强朱丽陈迎迎阮丽军张磊阮林高
Owner SHANGHAI LAIYI BIOMEDICAL RES & DEV CENT
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