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TSN (toosendanin) extraction method

An extraction method, the technology of toosendanin, applied in the direction of steroids, organic chemistry, etc., can solve the problems of low extraction yield, many impurities, and low purity, and achieve the effect of high purity, high yield, and improved purity

Inactive Publication Date: 2018-01-19
右江民族医学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the method has been improved to some extent, there are still problems such as low extraction rate, many impurities, and low purity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for extracting toosendanin, comprising the following steps:

[0028] (1): Wash the bark and bark of the neem tree, dry them, and crush them to 50 mesh;

[0029] (2): Put the pulverized material in step (1) into the dynamic countercurrent extraction equipment, add 80% ethanol, the ratio of solid to liquid is 1:10, and extract the 25min, filtered to obtain the crude extract;

[0030] (3): Add the natural traditional Chinese medicine clarifier KBT-ZTC produced by Kelier Biotechnology Co., Ltd. to the crude extract of step (2), the addition amount is 2g / L, when the flocs no longer increase, filter, and the filtrate Add 0.1g / L activated carbon to decolorize for 15min, centrifuge, and concentrate the supernatant by rotary evaporation to obtain extract;

[0031] (4): Dissolve the extract in step (3) with ethanol, and the solution is adsorbed by a macroporous adsorption resin. When desorbing, first use distilled water to remove impurities, and then use 60% methanol t...

Embodiment 2

[0036] A method for extracting toosendanin, comprising the following steps:

[0037] (1): Wash the bark and bark of the neem tree, dry them, and crush them to 80 mesh;

[0038] (2): Put the pulverized material in step (1) into the dynamic countercurrent extraction equipment, add 80% ethanol, the ratio of solid to liquid is 1:15, and extract the 15min, filtered to obtain the crude extract;

[0039] (3): Add the natural traditional Chinese medicine clarifier KBT-ZTC produced by Kelier Biotechnology Co., Ltd. to the crude extract of step (2), the addition amount is 5g / L, when the flocs no longer increase, filter, and the filtrate Add 0.3g / L activated carbon to decolorize for 20min, centrifuge, and concentrate the supernatant by rotary evaporation to obtain extract;

[0040] (4): Dissolve the extract in step (3) with ethanol, and the solution is adsorbed by a macroporous adsorption resin. When desorbing, first use distilled water to remove impurities, and then use 90% methanol t...

Embodiment 3

[0045] A method for extracting toosendanin, comprising the following steps:

[0046] (1): Wash the bark and bark of the neem tree, dry them, and crush them to 60 mesh;

[0047] (2): Put the pulverized material in step (1) into the dynamic countercurrent extraction equipment, add 80% ethanol, the ratio of solid to liquid is 1:12, and extract the 20min, filtered to obtain the crude extract;

[0048] (3): Add the natural traditional Chinese medicine clarifier KBT-ZTC produced by Kelier Biotechnology Co., Ltd. to the crude extract of step (2), the addition amount is 3g / L, when the flocs no longer increase, filter, and the filtrate Add 0.2g / L activated carbon to decolorize for 18min, centrifuge, and concentrate the supernatant by rotary evaporation to obtain extract;

[0049] (4): Dissolve the extract in step (3) with ethanol, and the solution is adsorbed by a macroporous adsorption resin. When desorbing, first use distilled water to remove impurities, and then use 80% methanol t...

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PUM

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Abstract

The invention discloses a TSN (toosendanin) extraction method. The method comprises the steps as follows: branch bark and bark of chinaberries are crushed and subjected to dynamic countercurrent extraction under ultrasonic assistance, a clarifying agent is added to an obtained extraction solution for impurity removal, decoloration is performed with activated carbon, an extract is obtained throughcondensation and passes macroporous adsorption resin, an obtained eluent is subjected to reduced pressure condensation, a crude extract is obtained, petroleum ether is added for precipitation after the crude extract is dissolved, a precipitate is washed, dried, dissolved and dried again, and TSN is obtained. The TSN is extracted through steps of ultrasonic-assisted dynamic countercurrent extraction, clarification with the clarifying agent, adsorption with the macroporous adsorption resin, petroleum ether precipitation and the like, the purity and extraction rate of the TSN can be effectively increased, and the extracted TSN is high in yield and purity and contains few impurities.

Description

technical field [0001] The invention relates to the technical field of extracting active ingredients of medicines, in particular to a method for extracting toosendanin. Background technique [0002] Toosendanin (Toosendanin, TSN, molecular formula is C 30 h 38 o 11 , molecular weight 574.60) is a tetracyclic triterpenoid compound extracted from the root bark, branch bark and fruit of Neem plants. It is a colorless needle-like crystal, easily soluble in methanol, ethanol, acetone, pyridine, slightly soluble in benzene And chloroform, almost insoluble in water and petroleum ether. TSN has a wide range of pharmacological effects and insecticidal activities. Its main pharmacological effects are: ascaris expelling, inhibition of the central nervous system, abnormal cardiac contraction rhythm, obvious impact on the digestive system, and anti-botulism effects. Recent studies have shown that TSN can induce cell differentiation and apoptosis, inhibit tumor cell proliferation, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J71/00
Inventor 黄锁义黄与路
Owner 右江民族医学院
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