101results about How to "Efficient separation and purification" patented technology

Methods and compositions are disclosed to engineer plastids comprising heterologous genes encoding immuno-activating domains fused to an extracellular domain (ECD) of a receptor or surface glycoprotein, a growth factor or an enzyme and produced within a subcellular organelle, such as a chloroplast. The immuno-activating domains may include those regions of a protein capable of modulating the interaction between immune effector cells via proteins containing stereoselective binding domains and specific ligands, such as the Fc regions of antibodies. The present disclosure also demonstrates the utility of plants, including green algae, for the production of complex multi-domain fusion proteins as soluble bioactive therapeutic agents.

The present invention relates to novel compositions of methotrexate-containing heterodimeric probe molecules, also known as chemical inducers of dimerization (CID), useful in three-hybrid assays. The invention further relates to synthesis of said compositions and their intermediates. Another aspect of the invention is a method for using the heterodimeric probe molecules described herein in drug screens to identify potential protein targets to a given ligand, optimize protein-ligand interactions, or identify potential ligands for a given protein target. In certain embodiments, the invention contemplates the synthesis of the following methotrexate-containing heterodimeric probe:

The invention provides a manufacturing technology of p-xylylene dichloride. The manufacturing technology comprises the following steps: (1) reaction: adding p-xylene in a chlorination reactor, turning on a reaction light source, and then turning on jacket steam of a reaction still to begin to heat and raise the temperature; when the temperature is raised to 80 DEG C, stopping steam heating, and introducing Cl2 to the reaction still; two hours after Cl2 introduction, increasing the chlorine flow, sampling to conduct center control analysis, and stopping introduction of the chlorine after meeting the process control requirements; (2) flash evaporation: simply distilling the mixed liquor obtained in the step (1), collecting the mixture of p-xylene and p-methylbenzyl chloride, and centrally collecting the bed charge inside the distillation still; (3) rectification: transferring the bed charge centrally collected in the step (2) to a benzylidene chloride rectifying column to be decompressed and rectified; collecting 4 mm Hg of fraction at 120-125 DEG C to obtain p-methylbenzylidene; collecting 4 mm Hg of fraction at 135-140 DEG C to obtain p-xylylene dichloride. The manufacturing technology disclosed by the invention can increase the yield of the product, achieves higher purity, and is easy for byproduct separation and purification.

The invention discloses a preparation method of high-purity algal polyphenol. The preparation method comprises the process steps of separation of algal polysaccharide and alga residues, enzymolysis wall breaking of the alga residues, efficient extraction of algal polyphenol, separation and purification of the algal polyphenol, and obtaining of the high-purity algal polyphenol. According to the preparation method, full-alga comprehensive utilization of the algal polysaccharide, the algal polyphenol and the alga residues is realized.

The invention discloses a micromolecule antibody affinity peptide and applications thereof. The amino acid sequence of the peptide is represented by any one of SEQ ID No. 1-112, and the peptide can combine with the Fc section of human immune globulin IgG, and does not combine with human serum albumin HAS. The provided micromolecule antibody affinity peptide can be taken as the affinity ligand to achieve the high efficient, safe, and economic IgG separation and purification.

The present invention relates to one kind of hybridized organic resin film and its preparation process. The hybridized organic resin film consists of polymer 10-25 wt%, ion exchange resin 5-40 wt%, solvent 30-80 wt%, and additive 5-30 wt%. Its preparation process includes the following steps: preparing film casting liquid according to the said recipe, filming and post-treatment. The hybridized organic resin film of the present invention has surface opening hole structure and excellent adsorbing performance, may be used as chromatographic medium and filtering medium and is especially suitable for use in separating and purifying protein.

The present invention belongs to the technical field of medicine, and relates to a high-speed countercurrent chromatography continuous circulation method for separating and preparing naphthoquinone compounds from Arnebia euchroma (Royle) Johnst. According to the method, the natural naphthoquinone compounds represented by a formula (I) are separated and prepared by using a high-speed countercurrent chromatography continuous circulation separation method, n-hexane, ethyl acetate, acetonitrile and water are used as a two-phase solvent system for high-speed countercurrent chromatography separation, an online storage technology and a circulation elution mode are combined, the fractions containing the target compound are subjected to continuous circulation elution, and the fractions are collected to obtain shikonin with a purity of more than 95%, propionylshikonin with a purity of more than 95%, deoxyshikonin with a purity of more than 95%, (beta,beta-dimethylacryl)shikonin with a purity of more than 95% and isovalerylshikonin with a purity of more than 95%. With the method of the present invention, the high purity naphthoquinone compounds can be obtained through the one separation purification, such that the method has advantages of simple operation, rapidness and high efficiency, and is suitable for the separation and preparation of naphthoquinone and a variety of natural compounds. The formula (I) is defined in the specification.

The invention relates to the field of molecular biology, in particular to a small molecular antibody affinity peptide with high antibody selectivity and application. The amino acid sequence of the small molecular antibody affinity peptide disclosed by the invention is shown as any of SEQ ID NO.1 to SEQ ID NO.54. The small molecular antibody affinity peptide according to the invention is combined with the Fc segment of immunoglobulin G (IgG), and is not combined with human serum albumin (HAS). When the small molecular antibody affinity peptide disclosed by the invention is taken as an affinity ligand, efficient, safe and economical IgG separation and purification can be realized.

The present invention relates to one kind of hybridized organic resin film and its preparation process. The hybridized organic resin film consists of polymer 10-25 wt%, ion exchange resin 5-40 wt%, solvent 30-80 wt%, and additive 5-30 wt%. Its preparation process includes the following steps: preparing film casting liquid according to the said recipe, filming and post-treatment. The hybridized organic resin film of the present invention has surface opening hole structure and excellent adsorbing performance, may be used as chromatographic medium and filtering medium and is especially suitable for use in separating and purifying protein.

The invention discloses a method for separating and purifying alpha-amylase by virtue of two aqueous phase extraction, and relates to a method for separating and purifying alpha-amylase. The method can be used for solving the problems of low sample recovery rate, expensive separation material or equipment, small sample processing amount and low separation purity of single method in the existing amylase separating method such as ammonium sulfate salting out method, centrifugal spray drying method and the like, as well as the defects that the alpha-amylase obtained by the ammonium sulfate salting out method contains more impurities, has low potency and cannot be directly applied to the food industry. The method for separating and purifying alpha-amylase by virtue of two-aqueous phase extraction comprises the following steps: (1) preparing a coarse enzyme liquid of fungus alpha-amylase; (2) preparing two aqueous phases of PEG (polyethylene glycol) / (NH4)2SO4; (3) extracting; and (4) purifying. The method disclosed by the invention can be used for separating and purifying alpha-amylase.

The invention relates to a preparation method for high-purity jasminoidin, particularly a preparation method for high-purity jasminoidin based on liquid-liquid partitioning extraction, which comprises the following steps: pulverizing gardenia, soaking in water, carrying out ultrasonic-assisted extraction, filtering, combining the extraction liquids, and concentrating while depressurizing to obtain a gardenia extraction concentrate; regulating the pH value of the gardenia extraction concentrate to alkaline, and extracting while stirring at high speed by using a low-carbon alcohol as a solvent, thus obtaining an extraction liquid rich in jasminoidin; decolorizing the obtained extraction liquid, and concentrating the decolorized extraction liquid while depressurizing to obtain a decolorized concentrate; pouring the decolorized concentrate into an organic acid ester while stirring so as to crystallize and precipitate jasminoidin; and separating out crystallized jasminoidin precipitate, washing with the organic acid ester, and repeatedly washing the precipitate to obtain the high-purity jasminoidin. The preparation method provided by the invention completely discards chromatography, has the advantages of simple operation process, short production period, high efficiency and high yield and purity of the product, and is suitable for industrial production.

The invention relates to a deep-eutectic-solvent based supported liquid membrane as well as a preparation method and application of the supported liquid membrane, belonging to the field of chemical separation. The supported liquid membrane comprises a porous support, and a liquid membrane phase is fixed on the pores and the surface of the supported liquid membrane, wherein the liquid membrane phase is a deep eutectic solvent formed by choline chloride and p-methylbenzene sulfonic acid at the molar ratio of 1:2, and the porous support is a hydrophilic mixed cellulose membrane. The supported liquid membrane provided by the invention has good stability and high tryptophan extraction efficiency.

The invention belongs to the field of biomass catalysis, and provides a method for preparing 5-hydroxymethylfurfural (5-HMF) by catalyzing cellulose transformation in a green solvent system. Based on a silicon-based mesoporous molecular sieve SBA-15, a magnetic carrier Fe3O4@SBA-15 is prepared, and the magnetic carrier Fe3O4@SBA-15 is used for preparing a biological enzyme catalyst cellulase-Fe3O4@SBA-15 through loading of cellulase; and through loading of ZrO2 / SO4<2-> type solid super acid, a 2SZ@SBA-15 chemical catalyst is successfully prepared. The two catalysts are coupled, the biological enzyme catalyst efficiently catalyzes and degrades the cellulose in an aqueous solution so that glucose is obtained, and in an isopropyl alcohol / water mixed solution, the chemical catalyst catalyzes and degrades the glucose obtained after a reaction so that the 5-HMF is prepared. The series connection of two steps of catalytic reactions can realize the high-efficient transformation from the cellulose to the 5-HMF in the green solvent system.

The invention provides an application of a protein A chromatography medium. The protein A chromatography medium is obtained by surface-hydrophilic dendrimer modification, activation and protein A coupling by taking a polymer microsphere containing a vinyl monomer as a raw material. When being used as an antibody and Fc fusion protein separation and purification medium, the protein A chromatography medium provided by the invention is capable of greatly increasing the flow rate, short in separation time, good in separation effect and low in cost so as to be suitable for rapid separation and purification of antibodies and Fc fusion proteins.

The present invention discloses a method for separation purification of Chinese magnoliavine fruit monomers by using a dynamic axial compression column. The method comprises the following steps: mobile phase preparation, dissolution filtration, dynamic axial compression column filling, system balancing, elution separation and concentration. According to the present invention, a plurality of monomers can be obtained during a separation purification process, purities detected by a liquid chromatography method are more than 98.5%, and yields are more than 98%; the process is simple, and the used organic solvents have characteristics of low price, low consumption and capability of recycling; an ultraviolet detector is adopted to perform online monitoring, operation is simple, efficiency is high, and product quality is easy to control; and isocratic elution is performed, performance requirements on equipment are low, a production cycle is short, and efficient Chinese magnoliavine fruit monomer separation purification from the laboratory to the industry can be easily achieved.

The invention relates the segregator, comprising permanent magnet components, cover (3), separating cap (4) and sample cap (5). In the separating cap (4) there is inner edge (6) which is connected with inner wall of separating cap (4); under interior extent (6) there is rubber ring (7), and between them there is filter paper; permanent magnet components comprises N magnet and S magnet; separating cap (4) is connected with sample cap (5). The invention solves the problem that the magnetic bacteria is not easy to separate, and the invention has the advantages of simple technology, low cost, easy usage and strong practicality.

The invention relates to an extraction method of hederagenin, and in the method, Chinese medicinal material teasel root is used as a raw material. The method comprises the following steps: a, adding hydrochloric acid or sulphuric acid into a 30-55% ethanol solution to prepare a 1.5-4N hydrochloric acid or sulphuric acid ethanol mixed solution; b, hydrolyzing the crushed teasel root in the hydrochloric acid or sulphuric acid ethanol mixed solution in the step a, filtering, and washing insoluble residues with water until the insoluble residues are neutral; c, extracting the residues obtained in the step b with an ethanol solution in a refluxing condition to obtain ethanol extract; and d, filtering the ethanol extract obtained in the step c through an ultrafiltration membrane with molecular weight cutoff of 3,000-8,000Da to obtain permeate, filtering the permeate with a nano-filtration membrane with a molecular weight cutoff of 200-600Da to obtain concentrate, and drying the concentrate to obtain the hederagenin. The method is simple and convenient in operation and simple and short in steps, so that the extraction quality and the extraction rate can be greatly improved.

A method for preparing high-content conjugated linoleic acid (CLA) through Purification of vegetable oil includes alcoholysis, purification and isomerization of vegetable oil. Alcoholysis is for preparing corresponding methyl ester or ethyl ester with glyceride; purification of methyl ester or ethyl ester is for obtaining methyl linoleate or ethyl linoleate of content over 85% through silver-based silica gel column chromatography; high-content CLA is obtained after alkali-catalyzed conjugation of methyl linoleate or ethyl linoleate, and CLA products are prepared as needed. This invention changes the status quo of preparing high-content CLA with safflower oil alone, expands sources of CLA, and develops an efficient technology for separation and purification of linoleic acid. The CLA obtained is of high purity and meets applications in pharmaceutical, health care products and other industries.

The invention discloses a method for preparing epoxydaucenal A and epoxydaucenal B. The method comprises the following steps: S1, performing extraction: crushing rosa rugosa var. plena Rehd dried in shade, performing extraction, performing filtration, and concentrating filtrate until alcohol taste does not exist; step S2, performing macroporous resin enrichment so as to obtain an enrichment substance freeze-dried powder rich in epoxydaucenal A and epoxydaucenal B; step S3, by using N-hexane / ethyl acetate / 95% ethanol / water in the volume ratio being 9 to 7 to 9 to 7 as a solvent system, performing HSCCC separation and purification so as to obtain the epoxydaucenal A and the epoxydaucenal B, wherein the purity of the epoxydaucenal A is 98.5%, and the purity of the epoxydaucenal B is 98.9%. Through adoption of the method provided by the invention, the epoxydaucenal A and the epoxydaucenal B can be efficiently prepared through separation and purification.

A process produces ε-caprolactone by the oxidation of cyclohexanone by feeding a crude reaction mixture to a first distillation column; distilling off a first distillate containing low boiling components including unreacted cyclohexanone from the top of the first distillation column; recovering a first side-cut fraction containing unreacted cyclohexanone in a higher concentration than in the first distillate from an intermediate tray; recovering a first bottom liquid containing high boiling components including ε-caprolactone from the bottom; introducing the first side-cut fraction to a second distillation column; recovering a second bottom liquid containing unreacted cyclohexanone from the bottom of the second distillation column; recycling the second bottom liquid into the raw material cyclohexanone; introducing the first bottom liquid to a third distillation column to thereby yield a third distillate containing ε-caprolactone from the third distillation column.

The invention discloses a method for separating and purifying pyrroloquinoline quinone in fermentation liquid by a molecularly imprinted solid-phase extraction method. According to the method, the pyrroloquinoline quinone is obtained by performing separation and purification on the fermentation liquid rich in the pyrroloquinoline quinone through a pyrroloquinoline quinone molecularly imprinted polymer. According to the method, silicon dioxide is used as a base material, the pyrroloquinoline quinone is used as a template molecule, methacrylic acid is used as a functional monomer, ethylene glycol dimethacrylate is used as a cross-linking agent, and the pyrroloquinoline quinone molecularly imprinted polymer is obtained through a thermal polymerization technique. Adsorptivity experiments prove that the pyrroloquinoline quinone molecularly imprinted polymer prepared by the method disclosed by the invention has good specific selective properties of adsorbing target objects namely the pyrroloquinoline quinone, and the largest adsorption volume can reach 10mg / mg (molecularly imprinted polymers). Solid-phase extraction is performed on the target object namely the pyrroloquinoline quinone by using the polymer as a solid-phase extraction filling, so that the purpose of efficiently separating and purifying the pyrroloquinoline quinone from the fermentation liquid is realized.

This invention relates to a separation and purification method for sponge dry cells-original cells, which utilizes four methods of differential centrifugation, specific agglutination of cells, differential adherence and centrifugation of density and grads together to increase the purity and efficiency of separation and purification of sponge original cells and provides an effective method for studying the biological property of exuberate film sponge dry cells and the vitro culture rule.

The invention relates to a method for preparing eskaserp molecular engram polymer, belonging to biological engineering technology. It comprises following steps: dissolving cross linker, functional monomer, mode molecule and initiator into pore-generating agent, getting mixing solvent; hypersonic de-gassing the mixing solvent; venting nitrogen after even mixing, sealing at nitrogen condition or vacuum condition; proceeding polymerization reaction, employing heat initiation for molecule engram polymer; grinding got molecule engram polymer after polymerization, sifting and removing mode molecule with organic solvent, checking with high efficiency liquid chromatography- ultraviolet until there is no mode molecule; vacuum drying and getting final product. The molecule engram polymer is characterized by simple, fast, high-effective separation and purification, enriching residual alserin, and direct usage for selective separation and enrichment alserin.

The invention discloses a method for separating and purifying deoxynucleoside triphosphate, which comprises the following steps: when the temperature is below 4 DEG C, depositing deoxynucleoside triphosphate biosynthesized reaction solution directly with an organic solvent to obtain a solid mixture of the deoxynucleoside triphosphate, wherein the volume ratio of the deoxynucleoside triphosphate biosynthesized reaction solution to the organic solvent is between 1:1 and 1:25; when the temperature is below the 4 DEG C, dissolving the solid mixture in ultrapure water, adjusting the pH value to between 0.1 and 2 by using acidic aqueous solution, and using the organic solvent to perform deposition again to obtain a solid matter of the deoxynucleoside triphosphate; and dissolving the solid matter in the ultrapure water, adjusting the pH value to between 5 and 11 by using sodium hydroxide alkaline aqueous solution, and performing liquid nitrogen frozen and freeze-drying to obtain a sodium salt product of the deoxynucleoside triphosphate. The method has the advantages of quick separation, good purification effect, production cost reduction, water resource pollution reduction, process energy consumption reduction, and applicability to industrial production.

The invention relates to the technical field of preparative liquid chromatography separation, and particularly discloses a two-dimensional high-pressure preparative liquid chromatography system and a separation and purification method for low-content target components in a medicine. The system comprises a first-dimensional high-pressure preparative liquid chromatography system, a two-position six-way switching valve, a multi-position selection valve, a trapping column and a second-dimensional high-pressure preparative liquid chromatography system, the second-dimensional high-pressure preparative liquid chromatography system comprises a second mixer and a two-dimensional separation column; the first-dimensional high-pressure preparative liquid chromatography system is sequentially communicated with the two-position six-way switching valve, the multi-position selection valve and the trapping column, and the outlet end of the trapping column is communicated with the two-position six-way switching valve again; and the second mixer is sequentially communicated with the two-position six-way switching valve and the two-dimensional separation column. The method comprises the following steps: detecting a sample by using a one-dimensional system, and trapping a target component to a trapping column; and switching the two-position six-way switching valve, back-washing out captured components, and carrying out two-dimensional detection. The method disclosed by the invention has the advantage of efficiently separating and purifying impurity components with extremely low content in the medicine.

The invention discloses a method for separating and purifying blood-glucose-reducing longan aril polysaccharides. The method comprises the following steps: (1) stirring and grinding; (2) preparing total extract of longan ari; (3) extracting; and (4) separating and purifying longan aril polysaccharides, namely enabling 500mL of longan aril crude polysaccharide solution to pass through filter membranes with molecular mass Mr of 30kD, 10kD, 3kD and 650D by adopting an ultrafiltration system, thereby respectively obtaining five longan aril polysaccharide solutions of which the Mr is more than 30kD, the Mr is more than 10kD and less than 30kD, the Mr is more than 3kD and less than 10kD, the Mr is more than 650D and less than 3kD and the Mr is less than 650D, wherein the polysaccharide with theMr of being more than 10kD and less than 30kD is the blood-glucose-reducing longan aril polysaccharide. According to the method disclosed by the invention, the blood-glucose-reducing longan aril polysaccharides can be efficiently separated and purified, the longan aril polysaccharides with different molecular weights can be prepared, and a research basis is provided for expanding further development of the longan aril polysaccharides.

The invention discloses a mutant protein A (Protein A) affinity chromatography medium, and the mutant protein A (Protein A) affinity chromatography medium as a chromatographic medium is used for separation and purification of antibodies and Fc fusion proteins. The mutant protein A (Protein A) affinity chromatography medium is prepared by modifying with a hydrophilic dendritic macromolecule, activating, coupling with a protein A (Protein A) ligand and capping based on genetically-engineered-modified Staphylococcus A protein, agarose or dextran gel microspheres. The modified protein A chromatographic medium has stable structure, moderate hardness, and a particle size distribution within a certain range. Therefore, modified protein A chromatographic medium has has good repeatability, high mechanical strength, greatly-improved flow rate, low cost, and large size when used in column loading, and is suitable for large-scale wide industrialization use.

The invention relates to a starlike-structured adsorption material and a preparation method thereof and belongs to the technical field of a preparation technology of a high polymer material. The starlike magnetic response material used for efficiently, rapidly and conveniently separating and purifying protein comprises raw materials as follows: a continuous phase and a dispersion phase, wherein the continuous phase comprises ethanol, toluene and polyvinylpyrrolidone, and the dispersion phase comprises GMA (glycidyl methacrylate), EDMA (ethylene dimethacrylate ), azodiisobutyronitrile, dodecanol, cyclohexanol and Fe3O4. Polymeric microspheres are controlled to perform surface crosslinking self-assembly with a dispersion polymerization method, so that a starlike magnetic response polymer adsorbent is formed. The prepared starlike magnetic response organic adsorbent not only has high specific surface area, high adsorption sites and high adsorbing capacity of a microsphere adsorbent, but also has the characteristics of large macro particle size, high magnetic response strength and the like, two characteristics including efficient adsorption and convenient separation of a biological macromolecular adsorbent are integrated, and a separation material with good performance is provided for the field of the bioseparation materials. The adsorption capacity of the material for BSA (bovineserum albumin) can reach 108 mg per g of a dry-based material.