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Preparation method of high-purity algal polyphenol

A seaweed polyphenol, high-purity technology, applied in the field of preparation of high-purity seaweed polyphenol, can solve the problems of long production time, high equipment requirements, complex production process, etc., to shorten production time, improve product quality, and simplify the process flow Effect

Active Publication Date: 2015-12-30
厦门爱逸零食研究所有限公司
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned method for preparing seaweed polyphenols lacks the comprehensive utilization of main components such as alginate, and the waste of resources is serious; at the same time, the above-mentioned process technology for preparing seaweed polyphenols also has the following problems: 1) The separation effect is poor and the purity is low
Seaweed polyphenols are easy to form complexes with seaweed gum and protein in the form of non-covalent bonds, and it is difficult to separate them in the subsequent purification process, resulting in a low recovery rate of seaweed polyphenols; in addition, the extracted seaweed polyphenols also contain A large amount of fat-soluble pigments cannot be effectively separated by traditional column chromatography, resulting in low purity of the product
2) Large structural damage and low activity
Although microwave technology and ultrasonic-assisted extraction technology can improve the yield of marine polyphenols, the extraction process will generate high temperature, coupled with the lack of effective protection measures, the oxidation of marine polyphenols will be accelerated under the action of oxygen, resulting in the loss of activity
3) The production process is complicated and the cost is high
The current process for processing seaweed polyphenols includes multiple processes such as crushing, extraction, centrifugation, filtration, column loading, elution, resin regeneration, multiple concentration, and drying; conventional column chromatography purification of seaweed polyphenols must also be equipped with a constant flow Pumps, glass columns, collection devices and other equipment lead to long production time and high equipment requirements, which limit the development of industrialization
This is the key technical bottleneck leading to the industrial application of seaweed polyphenols

Method used

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  • Preparation method of high-purity algal polyphenol
  • Preparation method of high-purity algal polyphenol

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Example 1: Preparation of high-purity seaweed polyphenols from kelp

[0027] (1) Get water content and be 20 mass % dry sea-tangle 1kg, after soaking and rinsing with tap water to remove impurities such as silt, beating with a beater to obtain 0.6L of seaweed slurry; send it into the agitator, and add 3L deionized water, under temperature Stir at a speed of 120 rpm for 30 minutes; then use a low-speed centrifuge to centrifuge the seaweed slurry at a speed of 1000 rpm for 5 minutes to collect the supernatant and seaweed residue; the seaweed residue is stirred by a mixer and centrifuged in a centrifuge according to the above steps and conditions Afterwards, the supernatant and seaweed residue were collected again. The two supernatants were combined and spray-dried to obtain seaweed polysaccharides; 342 g of seaweed residue was collected for extraction and preparation of seaweed polyphenols.

[0028] (2) Mix the wet kelp slag with 1.71 g of compound enzyme evenly, and hyd...

Embodiment 2

[0032] Embodiment 2: Preparation of high-purity seaweed polyphenols from wakame

[0033] (1) Take 1 kg of dried wakame with a water content of 20% by mass, soak, rinse, and beat with tap water to obtain 0.55 L of seaweed pulp; add 2.75 L of deionized water after sending it into the agitator, and stir at a speed of 120 rpm under temperature 30 min; then centrifuge at 1000 rpm for 5 min, collect the supernatant and seaweed residue respectively; repeat the above steps, the supernatant is used to prepare seaweed polysaccharide; collect 313 g of seaweed residue, which is used to extract and prepare seaweed polyphenols.

[0034] (2) Mix the above-mentioned wet wakame residue with 1.565 g of compound enzyme evenly, and hydrolyze it at room temperature for 5 hours, so as to break the cell wall of seaweed.

[0035] (3) 80% by volume ethanol aqueous solution containing 0.05% by mass, 0.01% by volume of phytic acid and 0.1% by volume of acetic acid is used as the extract. Add 1.565L of ...

Embodiment 3

[0038] Embodiment 3: Sea millet prepares high-purity seaweed polyphenols

[0039] (1) Take 1 kg of dry sea millet with a water content of 20% by mass, soak, rinse, and beat with tap water to obtain 0.5 L of seaweed slurry; add 2.5 L of deionized water after sending it into the agitator, and use 120 rpm under temperature. Stir for 30 minutes; then centrifuge at 1000 rpm for 5 minutes to collect the supernatant and seaweed residue; repeat the above steps, the supernatant is used to prepare seaweed polysaccharides; collect 410 g of seaweed residue, which is used to extract and prepare seaweed polyphenols.

[0040] (2) Mix the above-mentioned wet wakame residue with 2.05 g of compound enzyme evenly, and hydrolyze it at room temperature for 5 hours, so as to break the cell wall of seaweed.

[0041] (3) 80% by volume ethanol aqueous solution containing 0.05% by mass, 0.01% by volume of phytic acid and 0.1% by volume of acetic acid is used as the extract. Add 2.05L of the extract to...

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Abstract

The invention discloses a preparation method of high-purity algal polyphenol. The preparation method comprises the process steps of separation of algal polysaccharide and alga residues, enzymolysis wall breaking of the alga residues, efficient extraction of algal polyphenol, separation and purification of the algal polyphenol, and obtaining of the high-purity algal polyphenol. According to the preparation method, full-alga comprehensive utilization of the algal polysaccharide, the algal polyphenol and the alga residues is realized.

Description

technical field [0001] The invention relates to the technical field of seaweed polyphenols, in particular to a preparation method of high-purity seaweed polyphenols. Background technique [0002] Seaweed is the main source of marine plant polyphenols, and its structure is quite different from that of terrestrial plant polyphenols: terrestrial plant polyphenols are derived from gallic acid and halide acid; seaweed polyphenols are derivatives of phloroglucinol . Studies have shown that seaweed polyphenols have special effects in anti-oxidation, improving metabolic syndrome, and preventing degenerative diseases, and are safe and non-toxic. Therefore, European and American countries have invested heavily in the development and application of seaweed polyphenols. High-purity seaweed polyphenols have the advantages of strong biological activity, high medical value, and good stability, and are urgently needed products in the field of biomedicine. [0003] However, because seawee...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/03
Inventor 刘翼翔
Owner 厦门爱逸零食研究所有限公司
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