Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for separating and preparing natural naphthoquinone compounds

A compound and separation method technology, applied in the field of medicine, can solve the problems of difficulty in simultaneous separation and time-consuming, and achieve the effects of simple operation and high compound purity.

Inactive Publication Date: 2017-09-12
FUDAN UNIV
View PDF2 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it has been reported that naphthoquinone compounds, such as shikonin, β-hydroxyisovalerylshikonin, acetylshikonin, and isobutyrylshikonin, were isolated and purified from Sclerophylla sclerae by conventional HSCCC (Lu, et al, Journal of Chromatography A, 2004.1023 (1): 159-163; Feng, L, et al, Chromatographia, 2009.70 (7): 1197-1200.), but the conventional HSCCC method takes a long time and is difficult to separate Obtain high-purity monomeric compounds with similar structures

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for separating and preparing natural naphthoquinone compounds
  • Method for separating and preparing natural naphthoquinone compounds
  • Method for separating and preparing natural naphthoquinone compounds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) In this embodiment, the OptiChrome-300PLUS high-speed countercurrent chromatograph produced by Jiangyin Countercurrent Technology Co., Ltd. is improved, and the Agilent 1200HPLC high-performance liquid chromatograph produced by Agilent Co., Ltd. of the United States is used for purity analysis;

[0043] Set up a high-speed countercurrent chromatograph, in which, after the detector of the high-speed countercurrent chromatograph, flow valves a and b are connected, six-way valves a and b are respectively connected to storage rings I and II, and six-way valve c is connected in front of the constant flow pump, which is responsible for switching the normal washing Detachment mode and circulation elution mode, (the structure diagram of the high-speed countercurrent chromatograph of setting is as follows figure 1 shown);

[0044] (2) Preparation of extract samples

[0045] 54.8g of dried whole herb of Xinjiang comfrey was ultrasonically extracted three times with 500mL of ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention belongs to the technical field of medicine, and relates to a high-speed countercurrent chromatography continuous circulation method for separating and preparing naphthoquinone compounds from Arnebia euchroma (Royle) Johnst. According to the method, the natural naphthoquinone compounds represented by a formula (I) are separated and prepared by using a high-speed countercurrent chromatography continuous circulation separation method, n-hexane, ethyl acetate, acetonitrile and water are used as a two-phase solvent system for high-speed countercurrent chromatography separation, an online storage technology and a circulation elution mode are combined, the fractions containing the target compound are subjected to continuous circulation elution, and the fractions are collected to obtain shikonin with a purity of more than 95%, propionylshikonin with a purity of more than 95%, deoxyshikonin with a purity of more than 95%, (beta,beta-dimethylacryl)shikonin with a purity of more than 95% and isovalerylshikonin with a purity of more than 95%. With the method of the present invention, the high purity naphthoquinone compounds can be obtained through the one separation purification, such that the method has advantages of simple operation, rapidness and high efficiency, and is suitable for the separation and preparation of naphthoquinone and a variety of natural compounds. The formula (I) is defined in the specification.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a method for separating and preparing natural naphthoquinone compounds, in particular to preparing naphthoquinone compounds by adopting a high-speed countercurrent chromatography continuous circulation separation method. Background technique [0002] Arnebia euchroma (Royle) Johnst is a plant of the genus Arnebia euchroma (Royle) Johnst. Its dried roots are used as medicine and recorded in "Chinese Pharmacopoeia". ", listed as medium grade, sweet in the mouth, salty, cold in nature, GUIXIN, Liver Meridian. The traditional effects include clearing heat and cooling blood, promoting blood circulation, detoxifying and clearing rash. Chemical composition studies and modern pharmacological studies have shown that comfrey contains a variety of physiologically active components, which have been isolated from comfrey plants to naphthoquinones, benzoquinones, monoterpene phenol alkaloids, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07C50/32C07C46/10C07C69/30C07C67/58
CPCC07C46/10C07C67/58C07C50/32C07C69/30
Inventor 何建明穆青张诗悦
Owner FUDAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com