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Alserin molecular blotting polymer preparing method

A technology of molecular imprinting and polymers, which is applied in the field of bioengineering technology preparation, can solve the problems that the preparation method of reserpine molecular imprinting polymers has not yet been found, and achieve the effect of high selectivity and sensitivity

Inactive Publication Date: 2006-12-27
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] After checking the literature of the prior art, no relevant reports related to the preparation method of the reserpine molecularly imprinted polymer, the subject of the present invention, have been found so far

Method used

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  • Alserin molecular blotting polymer preparing method
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Examples

Experimental program
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Effect test

Embodiment 1

[0031] Dissolve 2 mmol of functional monomer methacrylic acid (MAA) and 1 mmol of template molecule reserpine in 5 ml of porogen chloroform, add 25 mmol of cross-linking agent ethylene glycol dimethacrylate (EGDMA), initiator azobisiso Nitrile (AIBN) ultrasonic degassing for 5 minutes, after mixing evenly, pass nitrogen gas for 10 minutes, and seal under nitrogen state or vacuum state. Reaction at 60°C for 36 hours; after the polymerization reaction, the synthesized polymer was taken out, ground, sieved, and template molecules were removed by Soxhlet extraction with methanol:acetic acid (80:20, V / V), and detected by high-performance liquid chromatography (UV). , until no template molecule is detected; the polymer from which the template molecule has been removed is vacuum-dried (50° C.) for 24 hours, and finally a molecularly imprinted polymer with an average particle size of 25-61 μm is obtained. The inventive effect is as follows: figure 1 , 2 shown.

Embodiment 2

[0033] Dissolve 5 mmol of functional monomer methacrylic acid (MAA) and 1 mmol of template molecule reserpine in 5 ml of porogen chloroform, add 25 mmol of crosslinking agent ethylene glycol dimethacrylate (EGDMA), initiator azobisiso Butyronitrile (AIBN) was ultrasonically degassed for 10 minutes. After mixing evenly, nitrogen gas was introduced for 15 minutes, and sealed under nitrogen or vacuum state. Reaction at 70°C for 20 hours; after the polymerization reaction, the synthesized polymer was taken out, ground, sieved, and template molecules were removed by Soxhlet extraction with methanol:acetic acid (80:20, V / V), and detected by high-performance liquid chromatography (UV). , until no template molecule is detected; the polymer from which the template molecule has been removed is vacuum-dried (50° C.) for 24 hours, and finally a molecularly imprinted polymer with an average particle size of 25-61 μm is obtained, and the inventive effect is basically equivalent to that of f...

Embodiment 3

[0035] Dissolve 8 mmol of functional monomer methacrylic acid (MAA) and 1 mmol of template molecule reserpine in 5 ml of porogen chloroform, add 25 mmol of crosslinking agent ethylene glycol dimethacrylate (EGDMA), initiator azobisiso Butyronitrile (AIBN) was ultrasonically degassed for 10 minutes. After mixing evenly, nitrogen gas was introduced for 15 minutes, and sealed under nitrogen or vacuum state. Reaction at 65°C for 28 hours; after the polymerization reaction, the synthesized polymer was taken out, ground, sieved, and template molecules were removed by Soxhlet extraction with methanol:acetic acid (80:20, V / V), and detected by high-performance liquid chromatography (UV). , until no template molecule is detected; the polymer from which the template molecule has been removed is vacuum-dried (50° C.) for 24 hours, and finally a molecularly imprinted polymer with an average particle size of 25-61 μm is obtained, and the inventive effect is basically equivalent to that of f...

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Abstract

The invention relates to a method for preparing eskaserp molecular engram polymer, belonging to biological engineering technology. It comprises following steps: dissolving cross linker, functional monomer, mode molecule and initiator into pore-generating agent, getting mixing solvent; hypersonic de-gassing the mixing solvent; venting nitrogen after even mixing, sealing at nitrogen condition or vacuum condition; proceeding polymerization reaction, employing heat initiation for molecule engram polymer; grinding got molecule engram polymer after polymerization, sifting and removing mode molecule with organic solvent, checking with high efficiency liquid chromatography- ultraviolet until there is no mode molecule; vacuum drying and getting final product. The molecule engram polymer is characterized by simple, fast, high-effective separation and purification, enriching residual alserin, and direct usage for selective separation and enrichment alserin.

Description

technical field [0001] The invention relates to a preparation method in the technical field of bioengineering, in particular to a preparation method of a reserpine molecularly imprinted polymer. Background technique [0002] Reserpine (RSP) is [11,17-dimethoxy-18-(3,4,5-trimethoxybenzoyl)oxy]yohenne-16 formate methyl ester, also known as blood Anping, serpentine, is the main alkaloid present in Rauwolfia produced in my country and India. It has the effect of lowering blood pressure and stabilizing. It is often used as a sedative to reduce the amount of exercise of livestock and poultry, so as to reduce the energy consumption of the body, The purpose of increasing energy accumulation, so as to achieve the effect of fattening and increasing the egg production rate of laying hens. As a drug, reserpine has good antihypertensive and sedative effects, but residues in animal-derived foods and aquatic products may cause damage to human health, such as long-term use of large doses can...

Claims

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Application Information

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IPC IPC(8): C08F20/06C08F20/18C08F12/08C08F2/44C08K5/10
Inventor 张大兵武爱波史西志李荣秀杨立桃
Owner SHANGHAI JIAO TONG UNIV
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