Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for dynamic countercurrent extraction of toosendanin

A dynamic countercurrent and toosendanin technology, applied in the direction of steroids, organic chemistry, etc., can solve the problems of insufficient separation and purification effect and low purity of toosendanin, so as to reduce the content of impurities, less impurities, and improve the extraction rate Effect

Inactive Publication Date: 2018-01-05
右江民族医学院
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method can increase the content of toosendanin through enzymolysis and dual-frequency ultrasonic extraction, the effect of separation and purification is insufficient, and the purity of toosendanin obtained is low

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for dynamic countercurrent extraction of toosendanin, comprising the following steps:

[0028] (1): Wash the bark and bark of the neem tree, dry them, and crush them to 60 mesh;

[0029] (2): Moisten the pulverized material in step (1) with 60% ethanol, put it into a microwave extraction equipment, treat it at a microwave power of 250 for 1 min, then put it into a dynamic countercurrent extraction equipment, add 80% ethanol, and the The material-liquid ratio of the pulverized material and 80% ethanol is 1:10, the extraction temperature is 30°C, the extraction is 15min, filtered, and the crude extract is obtained;

[0030] (3): Add natural Chinese medicine clarifier KBT-ZTC to the crude extract of step (2). The natural Chinese medicine clarifier KBT-ZTC is a clarifier produced by Kelier Biotechnology Co., Ltd., and the addition amount is 2g / L, when the flocs no longer increase, filter, add 0.1g / L activated carbon to the filtrate to decolorize for 15min, centri...

Embodiment 2

[0036] A method for dynamic countercurrent extraction of toosendanin, comprising the following steps:

[0037] (1): Wash the bark and bark of the neem tree, dry them, and crush them to 100 mesh;

[0038] (2): Wet the pulverized material in step (1) with 90% ethanol, put it into a microwave extraction equipment, and treat it at a microwave power of 350W for 3 minutes, then put it into a dynamic countercurrent extraction equipment, add 80% ethanol, and the The material-liquid ratio of the pulverized material and 80% ethanol is 1:15, the extraction temperature is 40°C, the extraction is carried out for 25 minutes, and filtered to obtain a crude extract;

[0039] (3): Add the natural Chinese medicine clarifier KBT-ZTC to the crude extract of step (2). The natural Chinese medicine clarifier KBT-ZTC is a clarifier produced by Kelier Biotechnology Co., Ltd., and the addition amount is 5g / L, when the flocs no longer increase, filter, add 0.3g / L activated carbon to the filtrate to de...

Embodiment 3

[0045] A method for dynamic countercurrent extraction of toosendanin, comprising the following steps:

[0046] (1): Wash the bark and bark of the neem tree, dry them, and crush them to 80 mesh;

[0047] (2): Wet the pulverized material in step (1) with 75% ethanol, put it into a microwave extraction equipment, treat it at a microwave power of 300W for 2 minutes, then put it into a dynamic countercurrent extraction equipment, add 80% ethanol, and the The material-liquid ratio of the pulverized material and 80% ethanol is 1:12, the extraction temperature is 35° C., extraction is carried out for 20 minutes, and the crude extract is obtained by filtration;

[0048](3): Add natural Chinese medicine clarifier KBT-ZTC to the crude extract of step (2). The natural Chinese medicine clarifier KBT-ZTC is a clarifier produced by Kelier Biotechnology Co., Ltd., and the addition amount is 3g / L, when the flocs no longer increase, filter, add 0.2g / L activated carbon to the filtrate to decol...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for dynamic countercurrent extraction of toosendanin. The method comprises the following steps: crushing branch skin and barks of melia azedarach; pre-treating the crushed branch skin and barks through microwaves; performing dynamic countercurrent extraction; adding a clarifying agent into an obtained extracting solution for impurity removal; decoloring the mixed solution with activated carbon; concentrating the mixed solution to obtain an extract; adsorbing the extract with macroporous adsorption resin, and concentrating obtained eluent through reduced pressure, so as to obtain a crude extract; after the crude extract is dissolved, adding petroleum ether for precipitation, washing precipitation with water, drying and re-dissolving the precipitation, so asto obtain the toosendanin. According to the method, the toosendanin is extracted by the steps of microwave pretreatment, dynamic countercurrent extraction, clarification with the clarifying agent, decoloration with the activated carbon, adsorption with the macroporous adsorption resin, precipitation with the petroleum ether and the like, so that the purity and the extraction efficiency of the toosendanin can be effectively improved, wherein the extraction efficiency is 1.3-1.6mg / g, and the purity is higher than 96%.

Description

technical field [0001] The invention relates to the technical field of extracting active ingredients of medicines, in particular to a method for dynamically countercurrently extracting toosendanin. Background technique [0002] Toosendanin (Toosendanin, TSN, molecular formula is C 30 h 38 o 11 , molecular weight 574.60) is a tetracyclic triterpenoid compound extracted from the root bark, branch bark and fruit of Neem plants. It is a colorless needle-like crystal, easily soluble in methanol, ethanol, acetone, pyridine, slightly soluble in benzene And chloroform, almost insoluble in water and petroleum ether. TSN has a wide range of pharmacological effects and insecticidal activities. Its main pharmacological effects are: ascaris expelling, inhibition of the central nervous system, abnormal cardiac contraction rhythm, obvious impact on the digestive system, and anti-botulism effects. Recent studies have shown that TSN can induce cell differentiation and apoptosis, inhibit ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07J71/00
Inventor 黄锁义黄与路
Owner 右江民族医学院
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com