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An anti-candida albicans composition containing sanguine and its preparation method

A technology of blood streptococcin and Candida albicans, which is applied in the direction of medical preparations containing active ingredients, medical preparations with non-active ingredients, antibacterial drugs, etc. The difficulty of clinical treatment of candidiasis and other problems, to achieve the effect of degradable safety and non-toxicity, relieve dry mouth, and degradation products are safe and non-toxic

Active Publication Date: 2020-09-29
马晟利
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The mechanical barrier formed by the extracellular matrix prevents drugs from penetrating through the biofilm to act on Candida albicans, especially the existing sprays, liniments and other dosage forms, which cannot stay on the surface of the affected area for a long time after being applied to the affected area. When the biofilm plays the role of medicine, it will be diluted or washed away by saliva, and the medicine effect cannot be fully exerted.
Therefore, growing in the form of biofilm and showing high drug resistance has brought great difficulties to the clinical treatment of oral candidiasis

Method used

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  • An anti-candida albicans composition containing sanguine and its preparation method

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Embodiment 1

[0033]An anti-Candida albicans composition containing streptococcin, which is a viscous gel dressing, in which the streptococcin is uniformly dispersed in the form of nanoscale liposomes, mainly including the following qualities Parts of components: 2-3 parts of streptococcin, 6-9 parts of PBS buffer solution, 10-1 parts of Tween 800.5 parts, 10-15 parts of polylactic acid-glycolic acid copolymer, 1-5 parts of collagen short peptide, vitamin 1-3 parts of B5, 1-3 parts of menthol, 1-2 parts of borneol, 2-5 parts of xylitol, 0.5-1 part of N-hydroxysuccinimide, 1-(3-dimethylaminopropyl)- 1-2 parts of 3-ethylcarbodiimide hydrochloride, 1.5-3 parts of dopamine and 100 parts of sodium hyaluronate aqueous solution with a mass concentration of 2-5%.

[0034] In this embodiment, streptococcin, PBS buffer solution, Tween 80 and polylactic acid-glycolic acid copolymer jointly form streptococcin liposome, and the drug loading rate of streptococcin in this liposome is 10~ 11%; Streptococc...

Embodiment 2

[0036] This embodiment provides an anti-Candida albicans composition containing streptococcin, which is a viscous gel dressing, and streptococcin is evenly dispersed in the viscous gel dressing in the form of nano-scale liposomes , mainly including the following components by mass: 3 parts of streptococcin, 9 parts of PBS buffer solution, 801 parts of Tween, 15 parts of polylactic acid-glycolic acid copolymer, 3 parts of collagen short peptide, 52 parts of vitamin B, menthol 2 parts, 1 part of borneol, 3 parts of xylitol, 1 part of N-hydroxysuccinimide, 2 parts of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, dopamine 3 parts and mass concentration are 100 parts of 5% sodium hyaluronate aqueous solution.

[0037] In this embodiment, streptococcin, PBS buffer solution, Tween 80 and polylactic acid-glycolic acid copolymer jointly constitute streptococcin liposome, and the drug loading rate of streptococcin in the liposome is 10.7%. ; Streptococcus liposomes and s...

Embodiment 3

[0039] This embodiment provides a method for preparing an anti-Candida albicans composition containing sanguine, comprising the steps of:

[0040] Step 1, extract blood streptococcin:

[0041] Proliferate and culture the purified and identified Streptococcus sanguis, inoculate the Streptococcus sanguis in BHI medium, and culture it under anaerobic conditions at 37°C for 48 hours, then centrifuge and collect the culture solution at 4°C. Wash bacterial sediment;

[0042] Disperse the obtained bacteria in PBS buffer solution to make a bacterial suspension, perform ultrasonic cell disruption on the bacterial suspension in an ice-water bath, and collect the supernatant of the cell disruption liquid by centrifugation at 4°C;

[0043] Ammonium sulfate was added to the supernatant of the resulting cell disruption liquid to make the ammonium sulfate in the supernatant reach a saturation of 60%, and it was placed at 4°C for salting out, and the first salting out solution was centrifuge...

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Abstract

The invention relates to an anti-candida-albicans composition containing streptocin and a preparation method thereof and belongs to the technical field of microbial pharmacy. In order to solve the problems that a biomembrane formed by candida albicans blocks drug permeation and reduces the medicinal effect, the anti-candida-albicans composition containing streptocin comprises medicinal componentsincluding streptocin, short collagen peptides, vitamins, menthol, borneol and xylitol. The composition is a viscous gel dressing and can be pasted to an affected mucous part for a long time to enablemore medicinal components to permeate through the biomembrane of candida albicans to take a medicinal effect, and therefore the problem of short acting time of existing dosage forms is solved. Streptocin is uniformly dispersed in the viscous gel dressing in the form of nano-scale liposome and more easily permeate through the biomembrane to directly act on candida albicans thalli, the medicinal effect is more thoroughly exerted, and the effects of reducing the adhesion of candida albicans and inhibiting and killing candida albicans can be rapidly achieved.

Description

technical field [0001] The invention belongs to the technical field of microbial pharmacy, and in particular relates to an anti-Candida albicans composition containing sanguine and a preparation method thereof. Background technique [0002] Candida albicans is a common conditional pathogen. Under the condition of the body’s normal functions, the pathogenic bacteria will not cause disease, but if the balance of oral flora is destroyed or the body’s immunity is reduced, Candida albicans can overgrow in the submucosal or even deep part of the oral cavity, causing the body to be infected with Candida albicans. In recent years, due to the increasing number of immunocompromised patients, such as the use of immunosuppressants, broad-spectrum antibiotics, and severe systemic diseases, the number of infections caused by opportunistic fungi has increased year by year, and pathogenic fungi are resistant to existing antifungal agents. Drug resistance has gradually developed, making fun...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/127A61K47/34A61K38/16A61P31/04
CPCA61K9/127A61K38/164A61K47/34A61P31/04
Inventor 马晟利李慧
Owner 马晟利
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